A0B2Z3 (METE_BURCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	Bcen2424_5286

Organism

Burkholderia cenocepacia (strain HI2424) [Complete proteome] [HAMAP]

Taxonomic identifier

331272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 764	764	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017230
Sites
	Metal binding	650	1	Zinc By similarity
	Metal binding	652	1	Zinc By similarity
	Metal binding	735	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0B2Z3 [UniParc]. Last modified November 28, 2006. Version 1. Checksum: A6E23B6F4D9AC516	FASTA	764	85,543
	10 20 30 40 50 60 MVTTHNLGFP RIGAKRELKF GLERYWKGES SRDELKALGA ELRRRHWHDQ RDLDLAPLGD 70 80 90 100 110 120 FAFYDQVLDM SFTLGNLPKR VQDFHGDALD NYFRVARGRS AQSAEEHAAC CGGVAAGEMT 130 140 150 160 170 180 KWFDTNYHYI VPEFHADTNF SLDPSRLLQQ LAEANAQGVN AKPVILGPVT YLWLGKAKDV 190 200 210 220 230 240 SDRLALLPKL LPVYGALLDT LTAQGVEWVQ IDEPILVTEL DAEWRQAFRT AYAALETRRI 250 260 270 280 290 300 KLLLATYFGQ LQDNLTLAAS LPVDGLHIDA INARDEVDAL VRELPAERVL SVGAINGRNI 310 320 330 340 350 360 WKTDLNAALD WLEPLAKQLG DRLWLAPSCS LLHVPVDLAS EEKLDAEIRS WLAFALQKLD 370 380 390 400 410 420 ELKVLATALN EGRDKVADAL AANAAAIDSR RRSPRVNNPV VKAAIARIDA QLGNRASPYT 430 440 450 460 470 480 QRASKQSARL NLPAFPTTTI GSFPQTAEIR QARSRFRAGA LDEAGYRTAM RAEIERSVRE 490 500 510 520 530 540 QESLELDVLV HGEAERNDMV EYFGEQLDGY AFSQFGWVQS YGSRCVKPPI LFGDISRPKA 550 560 570 580 590 600 MTVEWIAYAQ SLTRKPMKGM LTGPVTILNW SFVRDDQPRA VSCYQLALAI REEVLDLEKA 610 620 630 640 650 660 GVRVIQIDEA ALREGLPLRR AQWSEYLKWA VESFRITANG VQDDTQIHTH MCYSEFNDII 670 680 690 700 710 720 ASIADMDADV ITIETSRSDM ELLDAFDTFK YPNEIGPGVY DIHSPNIPTQ DHIVGLMKKA 730 740 750 760 AERIPAERLW VNPDCGLKTR QWAEVIPALT NMVAAAKTLR NQVQ

References

[1]

"Complete sequence of chromosome 2 of Burkholderia cenocepacia HI2424." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., LiPuma J.J., Gonzalez C.F., Konstantinidis K., Tiedje J.M., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HI2424.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000459 Genomic DNA. Translation: ABK12019.1.
RefSeq	YP_838912.1. NC_008543.1.
3D structure databases
ProteinModelPortal	A0B2Z3.
SMR	A0B2Z3. Positions 3-762.
ModBase	Search...
Protein-protein interaction databases
STRING	A0B2Z3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4453606.
GenomeReviews	Gene locus Bcen2424_5286 in contig CP000459_GR.
KEGG	bch:Bcen2424_5286.
PATRIC	19068199. VBIBurCen15205_5421.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0620.
HOGENOM	HBG287495.
OMA	RNIWRAN.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	BCEN331272:BCEN2424_5286-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BURCH

Accession

Primary (citable) accession number: A0B2Z3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 28, 2006
Last modified:	December 14, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program