A0B2Y5 (NADE_BURCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	Bcen2424_5278

Organism

Burkholderia cenocepacia (strain HI2424) [Complete proteome] [HAMAP]

Taxonomic identifier

331272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000099004
Regions
	Nucleotide binding	51 – 58	8	ATP By similarity
Sites
	Active site	53	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0B2Y5 [UniParc]. Last modified November 28, 2006. Version 1. Checksum: A98DDAE57566590A	FASTA	282	31,001
	10 20 30 40 50 60 MTSADYASRQ RAIIAELNVA PHFDAEAEIA RRIDFLAQYL RSTGLRTYVL GISGGVDSST 70 80 90 100 110 120 AGRLAQLSVE RLRADGYDAR FIAMRLPNGV QNDEEDAQRA LAFVRADEVL TVDVKPAADA 130 140 150 160 170 180 MLRSLVASGH AFETPAQQDF VHGNIKARER MIAQYAVAGA RRGIVIGTDH AAESLMGFFT 190 200 210 220 230 240 KFGDGGADIL PLAGLSKRRV RGVARALGGE ELIVMKVPTA DLEELRPLRP DEHAYGVTYD 250 260 270 280 EIDDFLEGKP VADRVYETVL RFYDGSRHKR ALPYTMFDWP AA

References

[1]

"Complete sequence of chromosome 2 of Burkholderia cenocepacia HI2424." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., LiPuma J.J., Gonzalez C.F., Konstantinidis K., Tiedje J.M., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HI2424.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000459 Genomic DNA. Translation: ABK12011.1.
RefSeq	YP_838904.1. NC_008543.1.
3D structure databases
ProteinModelPortal	A0B2Y5.
ModBase	Search...
Protein-protein interaction databases
STRING	A0B2Y5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4453598.
GenomeReviews	Gene locus Bcen2424_5278 in contig CP000459_GR.
KEGG	bch:Bcen2424_5278.
PATRIC	19068183. VBIBurCen15205_5413.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0171.
HOGENOM	HBG351567.
OMA	GLNKRRV.
ProtClustDB	PRK00768.
Enzyme and pathway databases
BioCyc	BCEN331272:BCEN2424_5278-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_BURCH

Accession

Primary (citable) accession number: A0B2Y5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 28, 2006
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program