Reviewed, UniProtKB/Swiss-Prot A0B2F7 (BETA_BURCH)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	Bcen2424_5100

Organism

Burkholderia cenocepacia (strain HI2424) [Complete proteome] [HAMAP]

Taxonomic identifier

331272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	566 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity.
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP MF_00750
Cofactor	FAD By similarity.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP MF_00750
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular alcohol metabolic process Inferred from electronic annotation. Source: InterPro glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 566	566	Choline dehydrogenase HAMAP MF_00750		PRO_1000046559
Regions
	Nucleotide binding	7 – 36	30	FAD Probable
Sites
	Active site	474	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0B2F7-1 [UniParc]. Last modified November 28, 2006. Version 1. Checksum: 76D9CF46E02C0724	FASTA	566	62,841
	10 20 30 40 50 60 MTTREYDYII CGAGSAGNVL ATRLTEDPDV TVLLLEAGGP DYRFDFRTQM PAALAYPLQG 70 80 90 100 110 120 RRYNWAYETD PEPHMDNRRM ECGRGKGLGG SSLINGMCYI RGNALDYDNW STHKGLENWT 130 140 150 160 170 180 YLDCLPYFKK AETRDVGPND YHGGSGPVSV TTSKPGVNPL FEAMVDAGVQ AGYPRTDDLN 190 200 210 220 230 240 GYQQEGFGPM DRTVTPKGRR ASTARGYLDQ AKVRPNLEIV THALADRILF DGKRASGVTY 250 260 270 280 290 300 LRGSERATAH ARREVLVCSG AIASPQLLQR SGVGPGAWLK ELDIPVVLDL PGVGQNLQDH 310 320 330 340 350 360 LEMYIQYECK EPVSLYPALK WWNQPKIGLE WMLNGTGLGA SNHFEAGGFI RTRDDDPWPN 370 380 390 400 410 420 IQYHFLPVAI NYNGSNAIEM HGFQAHVGSM RSPSRGRVKL RSRDPNDHPS ILFNYMAEAL 430 440 450 460 470 480 DWREFRDAIR ATREIMRQPA LDRYRGRELN PGADCKSDKE LDAFVRARAE TAFHPSCSCK 490 500 510 520 530 540 MGYDDMAVVD EEGRVHGLDG LRVVDASIMP IITTGNLNAP TIMIAEKIAD KIRGRTPLAR 550 560 VDVPYFVANG APARNVAKAV RQPETV

References

[1]

"Complete sequence of chromosome 2 of Burkholderia cenocepacia HI2424." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., LiPuma J.J., Gonzalez C.F., Konstantinidis K., Tiedje J.M., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000459 Genomic DNA. Translation: ABK11833.1.
RefSeq	YP_838726.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4453676.
GenomeReviews	Gene locus Bcen2424_5100 in contig CP000459_GR.
KEGG	bch:Bcen2424_5100.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	A0B2F7.
OMA	A0B2F7. PNLQYHF.
Family and domain databases
HAMAP	MF_00750. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_BURCH

Accession

Primary (citable) accession number: A0B2F7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 28, 2006
Last modified:	June 16, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)