Reviewed, UniProtKB/Swiss-Prot A0AXR0 (PANB2_BURCH)

Names and origin

Protein names

Recommended name:
    3-methyl-2-oxobutanoate hydroxymethyltransferase 2
    EC=2.1.2.11
Alternative name(s):
    Ketopantoate hydroxymethyltransferase 2
      Short name=KPHMT 2

Gene names

Name:	panB2
Ordered Locus Names:	Bcen2424_3447

Organism

Burkholderia cenocepacia (strain HI2424) [Complete proteome] [HAMAP]

Taxonomic identifier

331272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate. HAMAP MF_00156
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2. HAMAP MF_00156
Subunit structure	Homodecamer; pentamer of dimers By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the panB family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-methyl-2-oxobutanoate hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	3-methyl-2-oxobutanoate hydroxymethyltransferase 2 HAMAP MF_00156		PRO_0000297230
Regions
	Region	49 – 50	2	Alpha-ketoisovalerate binding By similarity
Sites
	Active site	187	1	Proton acceptor By similarity
	Metal binding	49	1	Magnesium By similarity
	Metal binding	88	1	Magnesium By similarity
	Metal binding	120	1	Magnesium By similarity
	Binding site	88	1	Alpha-ketoisovalerate By similarity
	Binding site	118	1	Alpha-ketoisovalerate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0AXR0-1 [UniParc]. Last modified November 28, 2006. Version 1. Checksum: 1C27E26A77B57D16	FASTA	284	29,919
	10 20 30 40 50 60 MSAHTRITRK TVTAIRSTKG IGSLVSLTAY SAPMAKLVDE VADVIIVGDS VGMVLYGMPD 70 80 90 100 110 120 TLRVTLDMMI AHGAAVVRGA AQACVVVDLP FSTFQESPAQ AYRSAARLLA ETGAQAVKLE 130 140 150 160 170 180 GGCEMTDTIR FLTDRGIPVM AHVGLMPQQA NAAGGFRAQG MDPRSAAQVF DAACAAERAG 190 200 210 220 230 240 AFSVVIEGTA EALARHLTET LTIPTIGIGA SPACDGQVLV TEDMIGAFDA YTPRFVKRYA 250 260 270 280 DANAVMRDAI RQYAHDVRQR VFPEPAHCFG YGKPLQLADA ATAA

References

[1]

"Complete sequence of chromosome 2 of Burkholderia cenocepacia HI2424." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., LiPuma J.J., Gonzalez C.F., Konstantinidis K., Tiedje J.M., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000459 Genomic DNA. Translation: ABK10186.1.
RefSeq	YP_837079.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0AXR0.
Genome annotation databases
GeneID	4451510.
GenomeReviews	Gene locus Bcen2424_3447 in contig CP000459_GR.
KEGG	bch:Bcen2424_3447.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	A0AXR0.
OMA	LVVVDMP.
Family and domain databases
HAMAP	MF_00156. [Tree]
InterPro	IPR003700. Pantoate_hydroxy_MeTrfase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
PANTHER	PTHR20881. Pantoate_transf. 1 hit.
Pfam	PF02548. Pantoate_transf. 1 hit. [Graphical view]
PIRSF	PIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
TIGRFAMs	TIGR00222. panB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANB2_BURCH

Accession

Primary (citable) accession number: A0AXR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	November 28, 2006
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)