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A0AVT1 (UBA6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-like modifier-activating enzyme 6

Short name=Ubiquitin-activating enzyme 6
Alternative name(s):
Monocyte protein 4
Short name=MOP-4
Ubiquitin-activating enzyme E1-like protein 2
Short name=E1-L2
Gene names
Name:UBA6
Synonyms:MOP4, UBE1L2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1052 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility. Ref.1 Ref.2 Ref.8

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Forms a thioester with UBD in cells stimulated with tumor necrosis factor-alpha (TNFa) and interferon-gamma (IFNg).

Tissue specificity

Widely expressed. Isoform 2 is predominantly expressed in testis with higher expression in adult testis than in fetal testis. Ref.1 Ref.2

Sequence similarities

Belongs to the ubiquitin-activating E1 family.

Sequence caution

The sequence BAA91824.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Note: Named isoforms=3.
Isoform 1 (identifier: A0AVT1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: A0AVT1-2)

Also known as: nUBE1L;

The sequence of this isoform differs from the canonical sequence as follows:
     1-474: Missing.
Isoform 3 (identifier: A0AVT1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     369-389: PDVNADIVHWLSWTAQGFLSP → VTIEIYGCPNICLLIHKCSVY
     390-1052: Missing.
Isoform 4 (identifier: A0AVT1-4)

The sequence of this isoform differs from the canonical sequence as follows:
     321-340: APLEIHTAMLALDQFQEKYS → VNKHFAGLREAAESEMRISE
     341-1052: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10521052Ubiquitin-like modifier-activating enzyme 6
PRO_0000277797

Regions

Nucleotide binding470 – 50031ATP By similarity

Sites

Active site6251Glycyl thioester intermediate By similarity

Amino acid modifications

Modified residue11N-acetylmethionine Ref.9
Modified residue5441N6-acetyllysine Ref.10
Modified residue7291N6-acetyllysine By similarity

Natural variations

Alternative sequence1 – 474474Missing in isoform 2.
VSP_023083
Alternative sequence321 – 34020APLEI…QEKYS → VNKHFAGLREAAESEMRISE in isoform 4.
VSP_023084
Alternative sequence341 – 1052712Missing in isoform 4.
VSP_023085
Alternative sequence369 – 38921PDVNA…GFLSP → VTIEIYGCPNICLLIHKCSV Y in isoform 3.
VSP_023086
Alternative sequence390 – 1052663Missing in isoform 3.
VSP_023087
Natural variant2241A → T. Ref.2 Ref.4 Ref.5
Corresponds to variant rs10010188 [ dbSNP | Ensembl ].
VAR_030594

Experimental info

Mutagenesis6251C → A or S: Impairs ubiquitin activation. Ref.2 Ref.8
Sequence conflict141E → K in CAD89908. Ref.5
Sequence conflict1401V → A in CAD89908. Ref.5
Sequence conflict1971C → Y in BAC04463. Ref.4
Sequence conflict2341N → D in CAD89959. Ref.5
Sequence conflict2971V → A in BAC04463. Ref.4
Sequence conflict6451F → V in AAQ63403. Ref.1
Sequence conflict8031Q → H in BAA91824. Ref.4
Sequence conflict8211E → G in CAD89959. Ref.5
Sequence conflict8681L → P in CAD89908. Ref.5
Sequence conflict9051V → G in AAQ63403. Ref.1
Sequence conflict9091M → K in CAD89959. Ref.5
Sequence conflict9201A → V in BAB19785. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 28, 2006. Version 1.
Checksum: 9F9C70EABA750A71

FASTA1,052117,970
        10         20         30         40         50         60 
MEGSEPVAAH QGEEASCSSW GTGSTNKNLP IMSTASVEID DALYSRQRYV LGDTAMQKMA 

        70         80         90        100        110        120 
KSHVFLSGMG GLGLEIAKNL VLAGIKAVTI HDTEKCQAWD LGTNFFLSED DVVNKRNRAE 

       130        140        150        160        170        180 
AVLKHIAELN PYVHVTSSSV PFNETTDLSF LDKYQCVVLT EMKLPLQKKI NDFCRSQCPP 

       190        200        210        220        230        240 
IKFISADVHG IWSRLFCDFG DEFEVLDTTG EEPKEIFISN ITQANPGIVT CLENHPHKLE 

       250        260        270        280        290        300 
TGQFLTFREI NGMTGLNGSI QQITVISPFS FSIGDTTELE PYLHGGIAVQ VKTPKTVFFE 

       310        320        330        340        350        360 
SLERQLKHPK CLIVDFSNPE APLEIHTAML ALDQFQEKYS RKPNVGCQQD SEELLKLATS 

       370        380        390        400        410        420 
ISETLEEKPD VNADIVHWLS WTAQGFLSPL AAAVGGVASQ EVLKAVTGKF SPLCQWLYLE 

       430        440        450        460        470        480 
AADIVESLGK PECEEFLPRG DRYDALRACI GDTLCQKLQN LNIFLVGCGA IGCEMLKNFA 

       490        500        510        520        530        540 
LLGVGTSKEK GMITVTDPDL IEKSNLNRQF LFRPHHIQKP KSYTAADATL KINSQIKIDA 

       550        560        570        580        590        600 
HLNKVCPTTE TIYNDEFYTK QDVIITALDN VEARRYVDSR CLANLRPLLD SGTMGTKGHT 

       610        620        630        640        650        660 
EVIVPHLTES YNSHRDPPEE EIPFCTLKSF PAAIEHTIQW ARDKFESSFS HKPSLFNKFW 

       670        680        690        700        710        720 
QTYSSAEEVL QKIQSGHSLE GCFQVIKLLS RRPRNWSQCV ELARLKFEKY FNHKALQLLH 

       730        740        750        760        770        780 
CFPLDIRLKD GSLFWQSPKR PPSPIKFDLN EPLHLSFLQN AAKLYATVYC IPFAEEDLSA 

       790        800        810        820        830        840 
DALLNILSEV KIQEFKPSNK VVQTDETARK PDHVPISSED ERNAIFQLEK AILSNEATKS 

       850        860        870        880        890        900 
DLQMAVLSFE KDDDHNGHID FITAASNLRA KMYSIEPADR FKTKRIAGKI IPAIATTTAT 

       910        920        930        940        950        960 
VSGLVALEMI KVTGGYPFEA YKNCFLNLAI PIVVFTETTE VRKTKIRNGI SFTIWDRWTV 

       970        980        990       1000       1010       1020 
HGKEDFTLLD FINAVKEKYG IEPTMVVQGV KMLYVPVMPG HAKRLKLTMH KLVKPTTEKK 

      1030       1040       1050 
YVDLTVSFAP DIDGDEDLPG PPVRYYFSHD TD 

« Hide

Isoform 2 (nUBE1L) [UniParc].

Checksum: 33FDBFF4EA8AADF0
Show »

FASTA57865,757
Isoform 3 [UniParc].

Checksum: E4534BE50710EF6C
Show »

FASTA38943,216
Isoform 4 [UniParc].

Checksum: B73DEE549B7E7143
Show »

FASTA34037,693

References

« Hide 'large scale' references
[1]"Identification and characteristics of a novel E1 like gene nUBE1L in human testis."
Zhu H., Zhou Z.-M., Huo R., Huang X.-Y., Lu L., Lin M., Wang L.-R., Zhou Y.-D., Li J.-M., Sha J.-H.
Acta Biochim. Biophys. Sin. 36:227-234(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, FUNCTION.
[2]"Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging."
Jin J., Li X., Gygi S.P., Harper J.W.
Nature 447:1135-1138(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT THR-224, FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF CYS-625.
[3]"Molecular and biological characterization of a new ubiquitin-activating enzyme E1 like protein, MOP-4 which is highly expressed in human monocytes."
Takayama K., Fujii Y., Ukai Y., Yoshimoto M.
Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Blood.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4), VARIANT THR-224.
Tissue: Hippocampus.
[5]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT THR-224.
Tissue: Skeletal muscle.
[6]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Tissue: Brain.
[8]"E1-L2 activates both ubiquitin and FAT10."
Chiu Y.-H., Sun Q., Chen Z.J.
Mol. Cell 27:1014-1023(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH UBD, THIOESTER FORMATION, MUTAGENESIS OF CYS-625.
[9]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-544, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY359880 mRNA. Translation: AAQ63403.1.
EF623993 mRNA. Translation: ABR25253.1.
AB014773 mRNA. Translation: BAB19785.1.
AK001670 mRNA. Translation: BAA91824.1. Different initiation.
AK094969 mRNA. Translation: BAC04463.1.
AK314371 mRNA. Translation: BAG37000.1.
AL832015 mRNA. Translation: CAD89908.1.
AL832458 mRNA. Translation: CAD89959.1.
AC079880 Genomic DNA. No translation available.
AC096720 Genomic DNA. Translation: AAY40999.1.
BC031637 mRNA. Translation: AAH31637.1.
BC126484 mRNA. Translation: AAI26485.1.
BC126486 mRNA. Translation: AAI26487.1.
CCDSCCDS3516.1. [A0AVT1-1]
RefSeqNP_060697.4. NM_018227.5. [A0AVT1-1]
UniGeneHs.212774.

3D structure databases

ProteinModelPortalA0AVT1.
SMRA0AVT1. Positions 38-1019.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120529. 34 interactions.
DIPDIP-57633N.
IntActA0AVT1. 1 interaction.
MINTMINT-1195700.

Chemistry

ChEMBLCHEMBL2321622.

PTM databases

PhosphoSiteA0AVT1.

Proteomic databases

MaxQBA0AVT1.
PaxDbA0AVT1.
PRIDEA0AVT1.

Protocols and materials databases

DNASU55236.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000322244; ENSP00000313454; ENSG00000033178. [A0AVT1-1]
ENST00000420827; ENSP00000399234; ENSG00000033178. [A0AVT1-3]
GeneID55236.
KEGGhsa:55236.
UCSCuc003hdg.4. human. [A0AVT1-1]
uc003hdi.3. human. [A0AVT1-3]
uc003hdj.2. human. [A0AVT1-4]

Organism-specific databases

CTD55236.
GeneCardsGC04M068481.
H-InvDBHIX0031574.
HIX0120163.
HGNCHGNC:25581. UBA6.
HPAHPA037001.
HPA044870.
MIM611361. gene.
neXtProtNX_A0AVT1.
PharmGKBPA162407690.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0476.
HOVERGENHBG054199.
InParanoidA0AVT1.
KOK10699.
OMAFSPLCQW.
OrthoDBEOG74R1PV.
PhylomeDBA0AVT1.
TreeFamTF300586.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.
UniPathwayUPA00143.

Gene expression databases

ArrayExpressA0AVT1.
BgeeA0AVT1.
GenevestigatorA0AVT1.

Family and domain databases

Gene3D1.10.3240.10. 2 hits.
3.40.50.720. 4 hits.
InterProIPR009036. Molybdenum_cofac_synth_MoeB.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
IPR018965. Ub-activating_enz_e1_C.
IPR023280. Ub-like_act_enz_cat_cys_dom.
IPR000127. UBact_repeat.
IPR019572. Ubiquitin-activating_enzyme.
IPR018075. UBQ-activ_enz_E1.
IPR000011. UBQ/SUMO-activ_enz_E1-like.
[Graphical view]
PfamPF00899. ThiF. 2 hits.
PF09358. UBA_e1_C. 1 hit.
PF10585. UBA_e1_thiolCys. 1 hit.
PF02134. UBACT. 1 hit.
[Graphical view]
PRINTSPR01849. UBIQUITINACT.
SMARTSM00985. UBA_e1_C. 1 hit.
[Graphical view]
SUPFAMSSF69572. SSF69572. 2 hits.
TIGRFAMsTIGR01408. Ube1. 1 hit.
ProtoNetSearch...

Other

ChiTaRSUBA6. human.
GeneWikiUBE1L2.
GenomeRNAi55236.
NextBio59255.
PROA0AVT1.
SOURCESearch...

Entry information

Entry nameUBA6_HUMAN
AccessionPrimary (citable) accession number: A0AVT1
Secondary accession number(s): A6N8M7 expand/collapse secondary AC list , B2RAV3, Q4W5K0, Q6UV21, Q86T78, Q86TC7, Q8N5T3, Q8N9E4, Q9H3T7, Q9NVC9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: November 28, 2006
Last modified: July 9, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM