A0AVK6 (E2F8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor E2F8
Short name=E2F-8

Gene names

Name:

E2F8

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	867 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Along with E2F7, inhibitor of E2F-dependent transcription. Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Directly represses E2F1 transcription. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession. Ref.5 Ref.6
Subunit structure	Forms heterodimers with E2F7 and, to a lesser extent, homodimers. Dimerization is important for DNA binding. Ref.7
Subcellular location	Nucleus Ref.6.
Domain	Both DNA-binding domains are required for DNA-binding and are proposed to form an intramolecular structure that is similar to the winged helix structure of the E2F-DP heterodimer By similarity.
Sequence similarities	Belongs to the E2F/DP family.
Sequence caution	The sequence AAH28244.2 differs from that shown. Reason: Erroneous initiation. The sequence BAB15605.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell cycle Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	DNA-binding
Molecular function	Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	transcription factor complex Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 867	867	Transcription factor E2F8		PRO_0000298909
Regions
	DNA binding	113 – 182	70	Potential
	DNA binding	261 – 347	87	Potential
Amino acid modifications
	Modified residue	357	1	Phosphoserine Ref.8
Natural variations
	Natural variant	674	1	I → V. Corresponds to variant rs793274 [ dbSNP | Ensembl ].		VAR_034735
Experimental info
	Mutagenesis	156	1	R → A: Loss of DNA-binding and inhibition of E2F1-dependent activation. Ref.6
	Mutagenesis	314	1	R → A: Loss of DNA-binding and inhibition of E2F1-dependent activation. Ref.6
	Sequence conflict	138	1	N → I in BAE46901. Ref.4
	Sequence conflict	460	1	K → R in BAB15605. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	A0AVK6 [UniParc]. Last modified November 28, 2006. Version 1. Checksum: AADBCFACB888BD93	FASTA	867	94,166
	10 20 30 40 50 60 MENEKENLFC EPHKRGLMKT PLKESTTANI VLAEIQPDFG PLTTPTKPKE GSQGEPWTPT 70 80 90 100 110 120 ANLKMLISAV SPEIRNRDQK RGLFDNRSGL PEAKDCIHEH LSGDEFEKSQ PSRKEKSLGL 130 140 150 160 170 180 LCHKFLARYP NYPNPAVNND ICLDEVAEEL NVERRRIYDI VNVLESLHMV SRLAKNRYTW 190 200 210 220 230 240 HGRHNLNKTL GTLKSIGEEN KYAEQIMMIK KKEYEQEFDF IKSYSIEDHI IKSNTGPNGH 250 260 270 280 290 300 PDMCFVELPG VEFRAASVNS RKDKSLRVMS QKFVMLFLVS TPQIVSLEVA AKILIGEDHV 310 320 330 340 350 360 EDLDKSKFKT KIRRLYDIAN VLSSLDLIKK VHVTEERGRK PAFKWTGPEI SPNTSGSSPV 370 380 390 400 410 420 IHFTPSDLEV RRSSKENCAK NLFSTRGKPN FTRHPSLIKL VKSIESDRRK INSAPSSPIK 430 440 450 460 470 480 TNKAESSQNS APFPSKMAQL AAICKMQLEE QSSESRQKVK VQLARSGPCK PVAPLDPPVN 490 500 510 520 530 540 AEMELTAPSL IQPLGMVPLI PSPLSSAVPL ILPQAPSGPS YAIYLQPTQA HQSVTPPQGL 550 560 570 580 590 600 SPTVCTTHSS KATGSKDSTD ATTEKAANDT SKASASTRPG SLLPAPERQG AKSRTREPAG 610 620 630 640 650 660 ERGSKRASML EDSGSKKKFK EDLKGLENVS ATLFPSGYLI PLTQCSSLGA ESILSGKENS 670 680 690 700 710 720 SALSPNHRIY SSPIAGVIPV TSSELTAVNF PSFHVTPLKL MVSPTSVAAV PVGNSPALAS 730 740 750 760 770 780 SHPVPIQNPS SAIVNFTLQH LGLISPNVQL SASPGSGIVP VSPRIESVNV APENAGTQQG 790 800 810 820 830 840 RATNYDSPVP GQSQPNGQSV AVTGAQQPVP VTPKGSQLVA ESFFRTPGGP TKPTSSSCMD 850 860 FEGANKTSLG TLFVPQRKLE VSTEDVH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Thymus.
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung, Mammary gland, Skin and Testis.
[4]	"Identification of novel human genes predicted by combining multiple gene finders." Totoki Y., Yada T., Sakaki Y., Takeda T. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-139.
[5]	"Characterization of E2F8, a novel E2F-like cell-cycle regulated repressor of E2F-activated transcription." Christensen J., Cloos P., Toftegaard U., Klinkenberg D., Bracken A.P., Trinh E., Heeran M., Di Stefano L., Helin K. Nucleic Acids Res. 33:5458-5470(2005) [PubMed: 16179649] [Abstract] Cited for: FUNCTION.
[6]	"E2F-8: an E2F family member with a similar organization of DNA-binding domains to E2F-7." Logan N., Graham A., Zhao X., Fisher R., Maiti B., Leone G., La Thangue N.B. Oncogene 24:5000-5004(2005) [PubMed: 15897886] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF ARG-156 AND ARG-314.
[7]	"Synergistic function of E2F7 and E2F8 is essential for cell survival and embryonic development." Li J., Ran C., Li E., Gordon F., Comstock G., Siddiqui H., Cleghorn W., Chen H.-Z., Kornacker K., Liu C.-G., Pandit S.K., Khanizadeh M., Weinstein M., Leone G., de Bruin A. Dev. Cell 14:62-75(2008) [PubMed: 18194653] [Abstract] Cited for: DIMERIZATION.
[8]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-357, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK026964 mRNA. Translation: BAB15605.1. Different initiation. AK292688 mRNA. Translation: BAF85377.1. CH471064 Genomic DNA. Translation: EAW68354.1. BC028244 mRNA. Translation: AAH28244.2. Different initiation. BC090877 mRNA. Translation: AAH90877.1. BC108700 mRNA. Translation: AAI08701.1. BC126400 mRNA. Translation: AAI26401.1. BC126402 mRNA. Translation: AAI26403.1. AB231781 mRNA. Translation: BAE46901.1.
IPI	IPI00296318.
RefSeq	NP_078956.2. NM_024680.2.
UniGene	Hs.523526.
3D structure databases
ProteinModelPortal	A0AVK6.
SMR	A0AVK6. Positions 112-183, 260-346.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-6541611.
STRING	A0AVK6.
PTM databases
PhosphoSite	A0AVK6.
Proteomic databases
PRIDE	A0AVK6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000250024; ENSP00000250024; ENSG00000129173.
GeneID	79733.
KEGG	hsa:79733.
UCSC	uc001mpm.1. human.
Organism-specific databases
CTD	79733.
GeneCards	GC11M019203.
H-InvDB	HIX0009498.
HGNC	HGNC:24727. E2F8.
MIM	612047. gene.
neXtProt	NX_A0AVK6.
PharmGKB	PA142671918.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG15825.
GeneTree	ENSGT00530000063616.
HOVERGEN	HBG063270.
InParanoid	A0AVK6.
OMA	ICKMQLE.
PhylomeDB	A0AVK6.
Gene expression databases
Bgee	A0AVK6.
CleanEx	HS_E2F8.
Genevestigator	A0AVK6.
Family and domain databases
InterPro	IPR015633. E2F. IPR003316. E2F_TDP. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 2 hits.
KO	K09391.
PANTHER	PTHR12081. E2F. 1 hit.
Pfam	PF02319. E2F_TDP. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	69118.
SOURCE	Search...

Entry information

Entry name

E2F8_HUMAN

Accession

Primary (citable) accession number: A0AVK6
Secondary accession number(s): A8K9H3 , Q2VPJ3, Q3C1U6, Q5BKY4, Q8N340, Q9H5M0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	November 28, 2006
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.