Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutathione biosynthesis bifunctional protein GshAB

Gene

gshAB

Organism
Listeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine.UniRule annotation

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation
ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi728 – 7281Magnesium or manganese 1UniRule annotation
Metal bindingi745 – 7451Magnesium or manganese 1UniRule annotation
Metal bindingi745 – 7451Magnesium or manganese 2UniRule annotation
Metal bindingi747 – 7471Magnesium or manganese 2UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi548 – 60659ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-cysteine ligase activity Source: UniProtKB-HAMAP
  3. glutathione synthase activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP
  5. manganese ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLWEL386043:GI5X-2800-MONOMER.
UniPathwayiUPA00142; UER00209.
UPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione biosynthesis bifunctional protein GshABUniRule annotation
Alternative name(s):
Gamma-GCS-GSUniRule annotation
Short name:
GCS-GSUniRule annotation
Including the following 2 domains:
Glutamate--cysteine ligaseUniRule annotation (EC:6.3.2.2UniRule annotation)
Alternative name(s):
Gamma-ECSUniRule annotation
Short name:
GCSUniRule annotation
Gamma-glutamylcysteine synthetaseUniRule annotation
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Alternative name(s):
GSH synthetaseUniRule annotation
Short name:
GSUniRule annotation
Short name:
GSH-SUniRule annotation
Short name:
GSHaseUniRule annotation
Glutathione synthaseUniRule annotation
Gene namesi
Name:gshABUniRule annotation
Synonyms:gshFUniRule annotation
Ordered Locus Names:lwe2717
OrganismiListeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334)
Taxonomic identifieri386043 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
ProteomesiUP000000779 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 776776Glutathione biosynthesis bifunctional protein GshABPRO_1000133720Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi386043.lwe2717.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini521 – 775255ATP-graspUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 354354Glutamate--cysteine ligaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1181.
HOGENOMiHOG000156471.
KOiK01919.
OMAiFNPSMYM.
OrthoDBiEOG6BKJ7H.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 3 hits.
HAMAPiMF_00782. Glut_biosynth.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
[Graphical view]
PfamiPF01071. GARS_A. 1 hit.
PF04262. Glu_cys_ligase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01435. glu_cys_lig_rel. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0AMA3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKLDMTILD SLKENKALRK LLFSGHFGLE KENIRVTSDG KLALTPHPAI
60 70 80 90 100
FGPKEDNPYI KTDFSESQIE MITPVTDSID DVYNWLENLH NIVSLRSKNE
110 120 130 140 150
LLWPSSNPPI LPAEKDIPIA EYKTPDSPDR KYREHLAQGY GKKIQLLSGI
160 170 180 190 200
HYNFSFPEAL IDGLYDEISL PNESKRDFKN RLYLKVAKYF MKNRWLLIYL
210 220 230 240 250
TGASPVYLAD FTKTKQEEKL RDGSSALHDG ISLRNSNAGY KNKESLYVDY
260 270 280 290 300
NSFDAYISSI SNYIEAGKIE SMREFYNPIR LKNAHTDQTV ESLAKHGVEY
310 320 330 340 350
LEIRSIDLNP LEPNGISKEA LHFIHLFLIK GLLSEDRELC ENNQQLADEN
360 370 380 390 400
ENNIALNGLS KPAIKNCDNE EMALADAGLL ELDKMNDFIQ SLRPEDTYFQ
410 420 430 440 450
AIIEKQKERL LHPEKTIAAQ VKEQSATAGF IEFHLNQAKT YMEETEALAY
460 470 480 490 500
KLIGAEDMEL STQIIWKDAI ARGIKVDVLD RAENFLRFQK GDHVEYVKQA
510 520 530 540 550
SKTSKDNYVS VLMMENKVVT KLVLAENNIR VPFGDSFSDQ ALALEAFSLF
560 570 580 590 600
KDKQIVVKPK STNYGWGISI FKNKFTTEDY QEALNIAFSY DSSVIIEEFI
610 620 630 640 650
PGDEFRFLVI NDKVEAVLKR VPANVTGDGI HTVRELVEEK NMDPLRGTDH
660 670 680 690 700
LKPLEKIRTG PEETLMLSMQ KLSWDSIPKA NETIYLRENS NVSTGGDSID
710 720 730 740 750
YTAEMDDYFK EIAIRATQVL DAKICGVDII VPRETIDRDK HAIIELNFNP
760 770
AMHMHCFPYQ GEQKKIGDKI LDFLFE
Length:776
Mass (Da):88,615
Last modified:November 28, 2006 - v1
Checksum:i9DDA45402F88588D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM263198 Genomic DNA. Translation: CAK22135.1.
RefSeqiWP_011703406.1. NC_008555.1.
YP_850914.1. NC_008555.1.

Genome annotation databases

EnsemblBacteriaiCAK22135; CAK22135; lwe2717.
KEGGilwe:lwe2717.
PATRICi20332701. VBILisWel39304_2724.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM263198 Genomic DNA. Translation: CAK22135.1.
RefSeqiWP_011703406.1. NC_008555.1.
YP_850914.1. NC_008555.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi386043.lwe2717.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAK22135; CAK22135; lwe2717.
KEGGilwe:lwe2717.
PATRICi20332701. VBILisWel39304_2724.

Phylogenomic databases

eggNOGiCOG1181.
HOGENOMiHOG000156471.
KOiK01919.
OMAiFNPSMYM.
OrthoDBiEOG6BKJ7H.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00209.
UPA00142; UER00210.
BioCyciLWEL386043:GI5X-2800-MONOMER.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 3 hits.
HAMAPiMF_00782. Glut_biosynth.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
[Graphical view]
PfamiPF01071. GARS_A. 1 hit.
PF04262. Glu_cys_ligase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01435. glu_cys_lig_rel. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Whole-genome sequence of Listeria welshimeri reveals common steps in genome reduction with Listeria innocua as compared to Listeria monocytogenes."
    Hain T., Steinweg C., Kuenne C.T., Billion A., Ghai R., Chatterjee S.S., Domann E., Kaerst U., Goesmann A., Bekel T., Bartels D., Kaiser O., Meyer F., Puehler A., Weisshaar B., Wehland J., Liang C., Dandekar T.
    , Lampidis R., Kreft J., Goebel W., Chakraborty T.
    J. Bacteriol. 188:7405-7415(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35897 / DSM 20650 / SLCC5334.

Entry informationi

Entry nameiGSHAB_LISW6
AccessioniPrimary (citable) accession number: A0AMA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 28, 2006
Last modified: April 1, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.