A0AKV5 (FABH_LISW6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxoacyl-[acyl-carrier-protein] synthase 3
EC=2.3.1.41

Alternative name(s):
3-oxoacyl-[acyl-carrier-protein] synthase III
Beta-ketoacyl-ACP synthase III
Short name=KAS III

Gene names

Name:	fabH
Ordered Locus Names:	lwe2219

Organism

Listeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334) [Complete proteome] [HAMAP]

Taxonomic identifier

386043 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids By similarity. HAMAP MF_01815
Catalytic activity	Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein]. HAMAP MF_01815
Pathway	Lipid metabolism; fatty acid biosynthesis. HAMAP MF_01815
Subunit structure	Homodimer By similarity. HAMAP MF_01815
Subcellular location	Cytoplasm Probable HAMAP MF_01815.
Domain	The last Arg residue of the ACP-binding site is essential for the weak association between ACP/AcpP and FabH By similarity. HAMAP MF_01815
Sequence similarities	Belongs to the FabH family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxoacyl-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	3-oxoacyl-[acyl-carrier-protein] synthase 3 HAMAP MF_01815		PRO_1000056374
Regions
	Region	238 – 242	5	ACP-binding By similarity
Sites
	Active site	112	1	By similarity
	Active site	237	1	By similarity
	Active site	267	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0AKV5 [UniParc]. Last modified November 28, 2006. Version 1. Checksum: 3402F87C2623930B	FASTA	312	34,029
	10 20 30 40 50 60 MNAGILGVGK YVPERILTNF DLEKMMETSD EWIRTRTGIE ERRIARDDEY THDLAYEAAK 70 80 90 100 110 120 VAIENAGLTP NDIDLFIVAT VTQEATFPSV ANIIQDRLGA KNAAGMDVEA ACAGFTFGVV 130 140 150 160 170 180 TAAQFIKTGA YKNIVVVGAD KLSKITNWDD RATAVLFGDG AGAIVMGPVS DDHGLLSFDL 190 200 210 220 230 240 GSDGSGGKYL NLDENKKIYM NGREVFRFAV RQMGEASLRV LERAGLEKED LDLLIPHQAN 250 260 270 280 290 300 IRIMEASRER LNLPEEKLMK TVHKYGNTSS SSIALALVDA VEEGRIKDND NVLLVGFGGG 310 LTWGALIIRW GK

References

[1]

"Whole-genome sequence of Listeria welshimeri reveals common steps in genome reduction with Listeria innocua as compared to Listeria monocytogenes." Hain T., Steinweg C., Kuenne C.T., Billion A., Ghai R., Chatterjee S.S., Domann E., Kaerst U., Goesmann A., Bekel T., Bartels D., Kaiser O., Meyer F., Puehler A., Weisshaar B., Wehland J., Liang C., Dandekar T. , Lampidis R., Kreft J., Goebel W., Chakraborty T. J. Bacteriol. 188:7405-7415(2006) [PubMed: 16936040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35897 / DSM 20650 / SLCC5334.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM263198 Genomic DNA. Translation: CAK21637.1.
RefSeq	YP_850416.1. NC_008555.1.
3D structure databases
ProteinModelPortal	A0AKV5.
SMR	A0AKV5. Positions 1-311.
ModBase	Search...
Protein-protein interaction databases
STRING	A0AKV5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4465223.
GenomeReviews	Gene locus lwe2219 in contig AM263198_GR.
KEGG	lwe:lwe2219.
NMPDR	fig|386043.6.peg.2158.
PATRIC	20331659. VBILisWel39304_2230.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0332.
HOGENOM	HBG649927.
OMA	KEIGAIN.
PhylomeDB	A0AKV5.
ProtClustDB	PRK09352.
Enzyme and pathway databases
BioCyc	LWEL386043:LWE2219-MONOMER.
Family and domain databases
HAMAP	MF_01815. FabH. [Tree]
InterPro	IPR013751. ACP_syn_III. IPR013747. ACP_syn_III_C. IPR004655. FabH_synth. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.
KO	K00648.
Pfam	PF08545. ACP_syn_III. 1 hit. PF08541. ACP_syn_III_C. 1 hit. [Graphical view]
SUPFAM	SSF53901. Thiolase-like. 1 hit.
TIGRFAMs	TIGR00747. FabH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABH_LISW6

Accession

Primary (citable) accession number: A0AKV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 28, 2006
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program