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A0AJB5 (SYL_LISW6) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:lwe1679
OrganismListeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334) [Complete proteome] [HAMAP]
Taxonomic identifier386043 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria

Protein attributes

Sequence length803 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 803803Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009367

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif575 – 5795"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5781ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A0AJB5 [UniParc].

Last modified November 28, 2006. Version 1.
Checksum: 17F8EA6EF2E90479

FASTA80392,069
        10         20         30         40         50         60 
MTFNHKKMEP KWQQYWSEHN TFKTTEDKDK ENFYALDMFP YPSGAGLHVG HPEGYTATDI 

        70         80         90        100        110        120 
LSRMKRMQGK NVLHPIGWDA FGLPAEQYAI DTGNDPEEFT ALNIANFTRQ IKSLGFSYDW 

       130        140        150        160        170        180 
DREINTTDPE YYKWTQWIFE KLYENGLAYE AEIAVNWCPA LGTVLANEEV IDGKSERGGF 

       190        200        210        220        230        240 
PVFRKPMRQW MLKITAYADR LLDDLDLVDW PENIKDMQRN WIGRSEGAEV TFKIKDSDET 

       250        260        270        280        290        300 
FNVFTTRPDT LFGATYTVFA PEHELIEKIT TPEQKEAVET YKKQVELKSE LERTDLAKDK 

       310        320        330        340        350        360 
TGVFTGAYAI NPINGEEVPI WIADYVLIQY GTGAIMAVPA HDERDFEFAQ QFGLNIRPVL 

       370        380        390        400        410        420 
EGGDVTKEAF TGDGPHINSE FLDGLAKEEA ITAAIDWLEK EGIGSRKITY RLRDWLFSRQ 

       430        440        450        460        470        480 
RYWGEPIPVI HWEDGETTLV PEEELPLLLP KATEIKPSGT GESPLANLHD WVNVTDESGR 

       490        500        510        520        530        540 
KGRRETNTMP QWAGSSWYFL RYIDPKNTEA IADKEKLIEW LPVDVYIGGA EHAVLHLLYA 

       550        560        570        580        590        600 
RFWHKFLYDI GVVPTKEPFQ KLFNQGMILG ENNEKMSKSR GNVVNPDEVV EKYGADTLRM 

       610        620        630        640        650        660 
YEMFMGPLDA SIAWNENGLE GARKFLDRIW RLFVTEEGIL AEKVTTTANA NLEKAYHHMV 

       670        680        690        700        710        720 
KTVTNHYENL RFNTGISQLM IFINEAYKQD SIPKEYVEGF VQLLSPIAPH LAEELWEILG 

       730        740        750        760        770        780 
HTETISYVTW PTYDETKLVE DEVEIVLQVN GKVKSKITVA KSLGKEELEK IAQEDDKMKE 

       790        800 
NLEGKTIRKV IVVPGKLVNI VGN 

« Hide

References

[1]"Whole-genome sequence of Listeria welshimeri reveals common steps in genome reduction with Listeria innocua as compared to Listeria monocytogenes."
Hain T., Steinweg C., Kuenne C.T., Billion A., Ghai R., Chatterjee S.S., Domann E., Kaerst U., Goesmann A., Bekel T., Bartels D., Kaiser O., Meyer F., Puehler A., Weisshaar B., Wehland J., Liang C., Dandekar T. expand/collapse author list , Lampidis R., Kreft J., Goebel W., Chakraborty T.
J. Bacteriol. 188:7405-7415(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35897 / DSM 20650 / SLCC5334.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM263198 Genomic DNA. Translation: CAK21097.1.
RefSeqYP_849876.1. NC_008555.1.

3D structure databases

ProteinModelPortalA0AJB5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING386043.lwe1679.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAK21097; CAK21097; lwe1679.
GeneID4465296.
KEGGlwe:lwe1679.
PATRIC20330509. VBILisWel39304_1689.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMADKPKYYA.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycLWEL386043:GI5X-1701-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_LISW6
AccessionPrimary (citable) accession number: A0AJB5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 28, 2006
Last modified: June 11, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries