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Protein

Glycerol kinase

Gene

glpK

Organism
Listeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.UniRule annotation

Catalytic activityi

ATP + glycerol = ADP + sn-glycerol 3-phosphate.UniRule annotation

Enzyme regulationi

Activated by phosphorylation and inhibited by fructose 1,6-bisphosphate (FBP).UniRule annotation

Pathwayi: glycerol degradation via glycerol kinase pathway

This protein is involved in step 1 of the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Glycerol kinase (glpK)
This subpathway is part of the pathway glycerol degradation via glycerol kinase pathway, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol, the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131SubstrateUniRule annotation
Binding sitei17 – 171ATPUniRule annotation
Binding sitei135 – 1351SubstrateUniRule annotation
Binding sitei267 – 2671ATPUniRule annotation
Binding sitei310 – 3101ATP; via carbonyl oxygenUniRule annotation
Binding sitei314 – 3141ATP; via amide nitrogenUniRule annotation
Binding sitei329 – 3291ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 153ATPUniRule annotation
Nucleotide bindingi411 – 4155ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycerol metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLWEL386043:GI5X-1573-MONOMER.
UniPathwayiUPA00618; UER00672.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol kinaseUniRule annotation (EC:2.7.1.30UniRule annotation)
Alternative name(s):
ATP:glycerol 3-phosphotransferaseUniRule annotation
GlycerokinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:glpKUniRule annotation
Ordered Locus Names:lwe1551
OrganismiListeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334)
Taxonomic identifieri386043 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
Proteomesi
  • UP000000779 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 497497Glycerol kinasePRO_1000020743Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei231 – 2311Phosphohistidine; by HPrUniRule annotation

Post-translational modificationi

The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), including enzyme I, and histidine-containing protein (HPr) are required for the phosphorylation, which leads to the activation of the enzyme.UniRule annotation

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homotetramer and homodimer (in equilibrium).UniRule annotation

Protein-protein interaction databases

STRINGi386043.lwe1551.

Structurei

3D structure databases

ProteinModelPortaliA0AIY7.
SMRiA0AIY7. Positions 5-490.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni83 – 842Substrate bindingUniRule annotation
Regioni245 – 2462Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the FGGY kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108HMR. Bacteria.
COG0554. LUCA.
HOGENOMiHOG000222134.
KOiK00864.
OMAiWWLTGGP.
OrthoDBiEOG6RZB46.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0AIY7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKKYILALD QGTTSSRAMI IDEEGEVIGV AQEEFDQIFP KPGWVEHSAN
60 70 80 90 100
EIWASILAVI AGVLLKTNIS SKEIAGIGIT NQRETTVIWD KESGNPIYNA
110 120 130 140 150
IVWQSRQTED ICKQLRKDGY EDTIRSKTGL LIDPYFAGTK ARWILDHVDG
160 170 180 190 200
AQERAEKGEL LFGTIDTWLV WKLTGGRAHI TDYSNASRTL LYNIYDLEWD
210 220 230 240 250
DELLKMLNIP RAMLPEVRPS SEVYADTVPY HFFGEEVPVA GIAGDQQAAL
260 270 280 290 300
FGQGCFEKGM AKNTYGTGCF LLMNTGEKAV RSENGLLTTL AWGIDGKVEY
310 320 330 340 350
ALEGSIFVAG SAIQWLRDGL RMVRQSSDSE NYASRIESSD GVYVVPAFVG
360 370 380 390 400
LGAPYWDSDV RGAVFGLTRG TEKEQFIRAT LESLAYQTRD VLYAMEQDSG
410 420 430 440 450
ISLKTLRVDG GASANNFLMQ FQSDILGVPV ERPENKETTV LGAAFLAGLA
460 470 480 490
VGVWKDKNEI KKHWKLDKRF EVEMKEEQRE DLYDGWHKAV KAAQAFK
Length:497
Mass (Da):55,450
Last modified:November 28, 2006 - v1
Checksum:iA141D6F6FF176000
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM263198 Genomic DNA. Translation: CAK20969.1.
RefSeqiWP_011702340.1. NC_008555.1.

Genome annotation databases

EnsemblBacteriaiCAK20969; CAK20969; lwe1551.
KEGGilwe:lwe1551.
PATRICi20330245. VBILisWel39304_1557.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM263198 Genomic DNA. Translation: CAK20969.1.
RefSeqiWP_011702340.1. NC_008555.1.

3D structure databases

ProteinModelPortaliA0AIY7.
SMRiA0AIY7. Positions 5-490.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi386043.lwe1551.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAK20969; CAK20969; lwe1551.
KEGGilwe:lwe1551.
PATRICi20330245. VBILisWel39304_1557.

Phylogenomic databases

eggNOGiENOG4108HMR. Bacteria.
COG0554. LUCA.
HOGENOMiHOG000222134.
KOiK00864.
OMAiWWLTGGP.
OrthoDBiEOG6RZB46.

Enzyme and pathway databases

UniPathwayiUPA00618; UER00672.
BioCyciLWEL386043:GI5X-1573-MONOMER.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Whole-genome sequence of Listeria welshimeri reveals common steps in genome reduction with Listeria innocua as compared to Listeria monocytogenes."
    Hain T., Steinweg C., Kuenne C.T., Billion A., Ghai R., Chatterjee S.S., Domann E., Kaerst U., Goesmann A., Bekel T., Bartels D., Kaiser O., Meyer F., Puehler A., Weisshaar B., Wehland J., Liang C., Dandekar T.
    , Lampidis R., Kreft J., Goebel W., Chakraborty T.
    J. Bacteriol. 188:7405-7415(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35897 / DSM 20650 / SLCC5334.

Entry informationi

Entry nameiGLPK_LISW6
AccessioniPrimary (citable) accession number: A0AIY7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 28, 2006
Last modified: November 11, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.