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A0AIC0

- SYP_LISW6

UniProt

A0AIC0 - SYP_LISW6

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Protein
Proline--tRNA ligase
Gene
proS, lwe1334
Organism
Listeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity.UniRule annotation

Catalytic activityi

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. proline-tRNA ligase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. prolyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLWEL386043:GI5X-1356-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Proline--tRNA ligase (EC:6.1.1.15)
Alternative name(s):
Prolyl-tRNA synthetase
Short name:
ProRS
Gene namesi
Name:proS
Ordered Locus Names:lwe1334
OrganismiListeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334)
Taxonomic identifieri386043 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
ProteomesiUP000000779: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 568568Proline--tRNA ligaseUniRule annotation
PRO_0000288344Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi386043.lwe1334.

Structurei

3D structure databases

ProteinModelPortaliA0AIC0.
SMRiA0AIC0. Positions 1-564.

Family & Domainsi

Domaini

Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0442.
HOGENOMiHOG000076893.
KOiK01881.
OMAiCDTIEKL.
OrthoDBiEOG6TTVMR.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
3.90.960.10. 1 hit.
HAMAPiMF_01569. Pro_tRNA_synth_type1.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023717. Pro-tRNA-Synthase_IIa_type1.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view]
PANTHERiPTHR11451:SF3. PTHR11451:SF3. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF04073. tRNA_edit. 1 hit.
[Graphical view]
PRINTSiPR01046. TRNASYNTHPRO.
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55826. SSF55826. 1 hit.
TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0AIC0-1 [UniParc]FASTAAdd to Basket

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MRQTMTFIPT LKEVPADAEV KSHQLLLRAG FIRQTASGIY SYLPLATLML    50
RKIEAIIREE LEAIGAAELL LPALQPAELW QESGRWNDYG PELMRLKDRA 100
SRDFALGPTH EEVITALLRD EVKSYKRLPL TLYQIQTKFR DEKRPRFGLL 150
RGREFIMKDA YSFHATSESL DEVYKLMHQA YSNIFTRCGL EFRSVIADSG 200
SIGGNESKEF MALSEIGEDT IAYSDASDYA ANTEMAPVLY MEKKSHELEK 250
ELEKVATANQ KFIADIVEFL EVPIEKTIKS MLYQVDEEVI MVLVRGDHEV 300
NDIKIKNALD ATNVELVDPA VPVEILGANF GSLGPIGVPE NIRVFADNAV 350
KDIANAVVGA NEDGYHYVNV NPNRDFEVTS YFDLRMIQAG DLSPDGQGVI 400
KFAEGIEVGH IFKLGTKYSE AMNATILDEN GRAQPIIMGC YGIGVSRILS 450
AIAEQSNDEN GLVWDKQISP FDLHLIPVNM KSEEQVAFAE SLYDSLQKAG 500
FSVLIDDRAE RAGVKFADAD LIGLPIRITV GKKAAEGIVE VKIRKTGEMI 550
EVRQDELLNT LPILFGDK 568
Length:568
Mass (Da):63,349
Last modified:November 28, 2006 - v1
Checksum:iD61C338686E421EF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM263198 Genomic DNA. Translation: CAK20752.1.
RefSeqiYP_849531.1. NC_008555.1.

Genome annotation databases

EnsemblBacteriaiCAK20752; CAK20752; lwe1334.
GeneIDi4465452.
KEGGilwe:lwe1334.
PATRICi20329811. VBILisWel39304_1340.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM263198 Genomic DNA. Translation: CAK20752.1 .
RefSeqi YP_849531.1. NC_008555.1.

3D structure databases

ProteinModelPortali A0AIC0.
SMRi A0AIC0. Positions 1-564.
ModBasei Search...

Protein-protein interaction databases

STRINGi 386043.lwe1334.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAK20752 ; CAK20752 ; lwe1334 .
GeneIDi 4465452.
KEGGi lwe:lwe1334.
PATRICi 20329811. VBILisWel39304_1340.

Phylogenomic databases

eggNOGi COG0442.
HOGENOMi HOG000076893.
KOi K01881.
OMAi CDTIEKL.
OrthoDBi EOG6TTVMR.

Enzyme and pathway databases

BioCyci LWEL386043:GI5X-1356-MONOMER.

Family and domain databases

Gene3Di 3.40.50.800. 1 hit.
3.90.960.10. 1 hit.
HAMAPi MF_01569. Pro_tRNA_synth_type1.
InterProi IPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023717. Pro-tRNA-Synthase_IIa_type1.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view ]
PANTHERi PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfami PF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF04073. tRNA_edit. 1 hit.
[Graphical view ]
PRINTSi PR01046. TRNASYNTHPRO.
SUPFAMi SSF52954. SSF52954. 1 hit.
SSF55826. SSF55826. 1 hit.
TIGRFAMsi TIGR00409. proS_fam_II. 1 hit.
PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Whole-genome sequence of Listeria welshimeri reveals common steps in genome reduction with Listeria innocua as compared to Listeria monocytogenes."
    Hain T., Steinweg C., Kuenne C.T., Billion A., Ghai R., Chatterjee S.S., Domann E., Kaerst U., Goesmann A., Bekel T., Bartels D., Kaiser O., Meyer F., Puehler A., Weisshaar B., Wehland J., Liang C., Dandekar T.
    , Lampidis R., Kreft J., Goebel W., Chakraborty T.
    J. Bacteriol. 188:7405-7415(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35897 / DSM 20650 / SLCC5334.

Entry informationi

Entry nameiSYP_LISW6
AccessioniPrimary (citable) accession number: A0AIC0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: November 28, 2006
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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