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Protein

Proline--tRNA ligase

Gene

proS

Organism
Listeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.UniRule annotation

Catalytic activityi

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLWEL386043:G1GJF-1360-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Proline--tRNA ligaseUniRule annotation (EC:6.1.1.15UniRule annotation)
Alternative name(s):
Prolyl-tRNA synthetaseUniRule annotation
Short name:
ProRSUniRule annotation
Gene namesi
Name:proSUniRule annotation
Ordered Locus Names:lwe1334
OrganismiListeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334)
Taxonomic identifieri386043 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
Proteomesi
  • UP000000779 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002883441 – 568Proline--tRNA ligaseAdd BLAST568

Proteomic databases

PRIDEiA0AIC0

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi386043.lwe1334

Structurei

3D structure databases

ProteinModelPortaliA0AIC0
SMRiA0AIC0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain.UniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C90 Bacteria
COG0442 LUCA
HOGENOMiHOG000076893
KOiK01881
OMAiIQPAELW
OrthoDBiPOG091H0290

Family and domain databases

CDDicd00779 ProRS_core_prok, 1 hit
Gene3Di3.40.50.800, 2 hits
3.90.960.10, 1 hit
HAMAPiMF_01569 Pro_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR002314 aa-tRNA-synt_IIb
IPR006195 aa-tRNA-synth_II
IPR004154 Anticodon-bd
IPR036621 Anticodon-bd_dom_sf
IPR002316 Pro-tRNA-ligase_IIa
IPR004500 Pro-tRNA-synth_IIa_bac-type
IPR023717 Pro-tRNA-Synthase_IIa_type1
IPR033730 ProRS_core_prok
IPR036754 YbaK/aa-tRNA-synt-asso_dom_sf
IPR007214 YbaK/aa-tRNA-synth-assoc-dom
PfamiView protein in Pfam
PF03129 HGTP_anticodon, 1 hit
PF00587 tRNA-synt_2b, 1 hit
PF04073 tRNA_edit, 1 hit
PIRSFiPIRSF001535 ProRS_1, 1 hit
PRINTSiPR01046 TRNASYNTHPRO
SUPFAMiSSF55826 SSF55826, 1 hit
TIGRFAMsiTIGR00409 proS_fam_II, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

A0AIC0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQTMTFIPT LKEVPADAEV KSHQLLLRAG FIRQTASGIY SYLPLATLML
60 70 80 90 100
RKIEAIIREE LEAIGAAELL LPALQPAELW QESGRWNDYG PELMRLKDRA
110 120 130 140 150
SRDFALGPTH EEVITALLRD EVKSYKRLPL TLYQIQTKFR DEKRPRFGLL
160 170 180 190 200
RGREFIMKDA YSFHATSESL DEVYKLMHQA YSNIFTRCGL EFRSVIADSG
210 220 230 240 250
SIGGNESKEF MALSEIGEDT IAYSDASDYA ANTEMAPVLY MEKKSHELEK
260 270 280 290 300
ELEKVATANQ KFIADIVEFL EVPIEKTIKS MLYQVDEEVI MVLVRGDHEV
310 320 330 340 350
NDIKIKNALD ATNVELVDPA VPVEILGANF GSLGPIGVPE NIRVFADNAV
360 370 380 390 400
KDIANAVVGA NEDGYHYVNV NPNRDFEVTS YFDLRMIQAG DLSPDGQGVI
410 420 430 440 450
KFAEGIEVGH IFKLGTKYSE AMNATILDEN GRAQPIIMGC YGIGVSRILS
460 470 480 490 500
AIAEQSNDEN GLVWDKQISP FDLHLIPVNM KSEEQVAFAE SLYDSLQKAG
510 520 530 540 550
FSVLIDDRAE RAGVKFADAD LIGLPIRITV GKKAAEGIVE VKIRKTGEMI
560
EVRQDELLNT LPILFGDK
Length:568
Mass (Da):63,349
Last modified:November 28, 2006 - v1
Checksum:iD61C338686E421EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM263198 Genomic DNA Translation: CAK20752.1
RefSeqiWP_011702137.1, NC_008555.1

Genome annotation databases

EnsemblBacteriaiCAK20752; CAK20752; lwe1334
KEGGilwe:lwe1334

Similar proteinsi

Entry informationi

Entry nameiSYP_LISW6
AccessioniPrimary (citable) accession number: A0AIC0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: November 28, 2006
Last modified: May 23, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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