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A0AIC0 (SYP_LISW6) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proline--tRNA ligase

EC=6.1.1.15
Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS
Gene names
Name:proS
Ordered Locus Names:lwe1334
OrganismListeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334) [Complete proteome] [HAMAP]
Taxonomic identifier386043 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria

Protein attributes

Sequence length568 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP-Rule MF_01569

Catalytic activity

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP-Rule MF_01569

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01569

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01569.

Domain

Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP-Rule MF_01569

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processprolyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

proline-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 568568Proline--tRNA ligase HAMAP-Rule MF_01569
PRO_0000288344

Sequences

Sequence LengthMass (Da)Tools
A0AIC0 [UniParc].

Last modified November 28, 2006. Version 1.
Checksum: D61C338686E421EF

FASTA56863,349
        10         20         30         40         50         60 
MRQTMTFIPT LKEVPADAEV KSHQLLLRAG FIRQTASGIY SYLPLATLML RKIEAIIREE 

        70         80         90        100        110        120 
LEAIGAAELL LPALQPAELW QESGRWNDYG PELMRLKDRA SRDFALGPTH EEVITALLRD 

       130        140        150        160        170        180 
EVKSYKRLPL TLYQIQTKFR DEKRPRFGLL RGREFIMKDA YSFHATSESL DEVYKLMHQA 

       190        200        210        220        230        240 
YSNIFTRCGL EFRSVIADSG SIGGNESKEF MALSEIGEDT IAYSDASDYA ANTEMAPVLY 

       250        260        270        280        290        300 
MEKKSHELEK ELEKVATANQ KFIADIVEFL EVPIEKTIKS MLYQVDEEVI MVLVRGDHEV 

       310        320        330        340        350        360 
NDIKIKNALD ATNVELVDPA VPVEILGANF GSLGPIGVPE NIRVFADNAV KDIANAVVGA 

       370        380        390        400        410        420 
NEDGYHYVNV NPNRDFEVTS YFDLRMIQAG DLSPDGQGVI KFAEGIEVGH IFKLGTKYSE 

       430        440        450        460        470        480 
AMNATILDEN GRAQPIIMGC YGIGVSRILS AIAEQSNDEN GLVWDKQISP FDLHLIPVNM 

       490        500        510        520        530        540 
KSEEQVAFAE SLYDSLQKAG FSVLIDDRAE RAGVKFADAD LIGLPIRITV GKKAAEGIVE 

       550        560 
VKIRKTGEMI EVRQDELLNT LPILFGDK 

« Hide

References

[1]"Whole-genome sequence of Listeria welshimeri reveals common steps in genome reduction with Listeria innocua as compared to Listeria monocytogenes."
Hain T., Steinweg C., Kuenne C.T., Billion A., Ghai R., Chatterjee S.S., Domann E., Kaerst U., Goesmann A., Bekel T., Bartels D., Kaiser O., Meyer F., Puehler A., Weisshaar B., Wehland J., Liang C., Dandekar T. expand/collapse author list , Lampidis R., Kreft J., Goebel W., Chakraborty T.
J. Bacteriol. 188:7405-7415(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35897 / DSM 20650 / SLCC5334.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM263198 Genomic DNA. Translation: CAK20752.1.
RefSeqYP_849531.1. NC_008555.1.

3D structure databases

ProteinModelPortalA0AIC0.
SMRA0AIC0. Positions 1-564.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING386043.lwe1334.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAK20752; CAK20752; lwe1334.
GeneID4465452.
KEGGlwe:lwe1334.
PATRIC20329811. VBILisWel39304_1340.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0442.
HOGENOMHOG000076893.
KOK01881.
OMAIQPAELW.
OrthoDBEOG6TTVMR.
ProtClustDBPRK09194.

Enzyme and pathway databases

BioCycLWEL386043:GI5X-1356-MONOMER.

Family and domain databases

Gene3D3.40.50.800. 1 hit.
3.90.960.10. 1 hit.
HAMAPMF_01569. Pro_tRNA_synth_type1.
InterProIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023717. Pro-tRNA-Synthase_IIa_type1.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view]
PANTHERPTHR11451:SF3. PTHR11451:SF3. 1 hit.
PfamPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF04073. tRNA_edit. 1 hit.
[Graphical view]
PRINTSPR01046. TRNASYNTHPRO.
SUPFAMSSF52954. SSF52954. 1 hit.
SSF55826. SSF55826. 1 hit.
TIGRFAMsTIGR00409. proS_fam_II. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYP_LISW6
AccessionPrimary (citable) accession number: A0AIC0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: November 28, 2006
Last modified: April 16, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries