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A0AIC0

- SYP_LISW6

UniProt

A0AIC0 - SYP_LISW6

Protein

Proline--tRNA ligase

Gene

proS

Organism
Listeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (28 Nov 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.UniRule annotation

    Catalytic activityi

    ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. proline-tRNA ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. prolyl-tRNA aminoacylation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciLWEL386043:GI5X-1356-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proline--tRNA ligaseUniRule annotation (EC:6.1.1.15UniRule annotation)
    Alternative name(s):
    Prolyl-tRNA synthetaseUniRule annotation
    Short name:
    ProRSUniRule annotation
    Gene namesi
    Name:proSUniRule annotation
    Ordered Locus Names:lwe1334
    OrganismiListeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334)
    Taxonomic identifieri386043 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
    ProteomesiUP000000779: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 568568Proline--tRNA ligasePRO_0000288344Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi386043.lwe1334.

    Structurei

    3D structure databases

    ProteinModelPortaliA0AIC0.
    SMRiA0AIC0. Positions 1-564.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain.UniRule annotation

    Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0442.
    HOGENOMiHOG000076893.
    KOiK01881.
    OMAiCDTIEKL.
    OrthoDBiEOG6TTVMR.

    Family and domain databases

    Gene3Di3.40.50.800. 1 hit.
    3.90.960.10. 1 hit.
    HAMAPiMF_01569. Pro_tRNA_synth_type1.
    InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002316. Pro-tRNA-ligase_IIa.
    IPR004500. Pro-tRNA-synth_IIa_bac-type.
    IPR023717. Pro-tRNA-Synthase_IIa_type1.
    IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
    [Graphical view]
    PANTHERiPTHR11451:SF3. PTHR11451:SF3. 1 hit.
    PfamiPF03129. HGTP_anticodon. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    PF04073. tRNA_edit. 1 hit.
    [Graphical view]
    PRINTSiPR01046. TRNASYNTHPRO.
    SUPFAMiSSF52954. SSF52954. 1 hit.
    SSF55826. SSF55826. 1 hit.
    TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A0AIC0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRQTMTFIPT LKEVPADAEV KSHQLLLRAG FIRQTASGIY SYLPLATLML    50
    RKIEAIIREE LEAIGAAELL LPALQPAELW QESGRWNDYG PELMRLKDRA 100
    SRDFALGPTH EEVITALLRD EVKSYKRLPL TLYQIQTKFR DEKRPRFGLL 150
    RGREFIMKDA YSFHATSESL DEVYKLMHQA YSNIFTRCGL EFRSVIADSG 200
    SIGGNESKEF MALSEIGEDT IAYSDASDYA ANTEMAPVLY MEKKSHELEK 250
    ELEKVATANQ KFIADIVEFL EVPIEKTIKS MLYQVDEEVI MVLVRGDHEV 300
    NDIKIKNALD ATNVELVDPA VPVEILGANF GSLGPIGVPE NIRVFADNAV 350
    KDIANAVVGA NEDGYHYVNV NPNRDFEVTS YFDLRMIQAG DLSPDGQGVI 400
    KFAEGIEVGH IFKLGTKYSE AMNATILDEN GRAQPIIMGC YGIGVSRILS 450
    AIAEQSNDEN GLVWDKQISP FDLHLIPVNM KSEEQVAFAE SLYDSLQKAG 500
    FSVLIDDRAE RAGVKFADAD LIGLPIRITV GKKAAEGIVE VKIRKTGEMI 550
    EVRQDELLNT LPILFGDK 568
    Length:568
    Mass (Da):63,349
    Last modified:November 28, 2006 - v1
    Checksum:iD61C338686E421EF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM263198 Genomic DNA. Translation: CAK20752.1.
    RefSeqiYP_849531.1. NC_008555.1.

    Genome annotation databases

    EnsemblBacteriaiCAK20752; CAK20752; lwe1334.
    GeneIDi4465452.
    KEGGilwe:lwe1334.
    PATRICi20329811. VBILisWel39304_1340.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM263198 Genomic DNA. Translation: CAK20752.1 .
    RefSeqi YP_849531.1. NC_008555.1.

    3D structure databases

    ProteinModelPortali A0AIC0.
    SMRi A0AIC0. Positions 1-564.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 386043.lwe1334.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAK20752 ; CAK20752 ; lwe1334 .
    GeneIDi 4465452.
    KEGGi lwe:lwe1334.
    PATRICi 20329811. VBILisWel39304_1340.

    Phylogenomic databases

    eggNOGi COG0442.
    HOGENOMi HOG000076893.
    KOi K01881.
    OMAi CDTIEKL.
    OrthoDBi EOG6TTVMR.

    Enzyme and pathway databases

    BioCyci LWEL386043:GI5X-1356-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.800. 1 hit.
    3.90.960.10. 1 hit.
    HAMAPi MF_01569. Pro_tRNA_synth_type1.
    InterProi IPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002316. Pro-tRNA-ligase_IIa.
    IPR004500. Pro-tRNA-synth_IIa_bac-type.
    IPR023717. Pro-tRNA-Synthase_IIa_type1.
    IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
    [Graphical view ]
    PANTHERi PTHR11451:SF3. PTHR11451:SF3. 1 hit.
    Pfami PF03129. HGTP_anticodon. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    PF04073. tRNA_edit. 1 hit.
    [Graphical view ]
    PRINTSi PR01046. TRNASYNTHPRO.
    SUPFAMi SSF52954. SSF52954. 1 hit.
    SSF55826. SSF55826. 1 hit.
    TIGRFAMsi TIGR00409. proS_fam_II. 1 hit.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Whole-genome sequence of Listeria welshimeri reveals common steps in genome reduction with Listeria innocua as compared to Listeria monocytogenes."
      Hain T., Steinweg C., Kuenne C.T., Billion A., Ghai R., Chatterjee S.S., Domann E., Kaerst U., Goesmann A., Bekel T., Bartels D., Kaiser O., Meyer F., Puehler A., Weisshaar B., Wehland J., Liang C., Dandekar T.
      , Lampidis R., Kreft J., Goebel W., Chakraborty T.
      J. Bacteriol. 188:7405-7415(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35897 / DSM 20650 / SLCC5334.

    Entry informationi

    Entry nameiSYP_LISW6
    AccessioniPrimary (citable) accession number: A0AIC0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: November 28, 2006
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3