A0AI65 (PROB_LISW6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate 5-kinase
EC=2.7.2.11

Alternative name(s):
Gamma-glutamyl kinase
Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	lwe1279

Organism

Listeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334) [Complete proteome] [HAMAP]

Taxonomic identifier

386043 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Listeriaceae › Listeria › Listeria welshimeri

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00456.
Sequence similarities	Belongs to the glutamate 5-kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 276	276	Glutamate 5-kinase HAMAP-Rule MF_00456		PRO_1000081070
Regions
	Nucleotide binding	177 – 178	2	ATP By similarity
	Nucleotide binding	219 – 225	7	ATP By similarity
Sites
	Binding site	14	1	ATP By similarity
	Binding site	54	1	Substrate By similarity
	Binding site	141	1	Substrate By similarity
	Binding site	157	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0AI65 [UniParc]. Last modified November 28, 2006. Version 1. Checksum: 7F45859526475E2C	FASTA	276	30,110
	10 20 30 40 50 60 MRESLKNSKR LVIKVGTSTL MYGNGHVNLR TIEKLAMVLS DLRNEGKEVI LVSSGAIGVG 70 80 90 100 110 120 CHKLQLPARP TDIPDLQAVA SVGQSELMHI YSKFFGEYGQ VVGQVLLTRD VTDFPISREN 130 140 150 160 170 180 VMNTLESLLS RGIIPIVNEN DTVAVEELEH VTKYGDNDLL SAIVARLVQA DLLIMLSDID 190 200 210 220 230 240 GFYKSNPTTD PNAIMFSEIN QITPEIEALA GGRGSKFGTG GMLTKLSAAT YCMASHQKMI 250 260 270 LTNGKNPTII FDIMQGAQVG TLFANKKEAF SHDRTH

References

[1]

"Whole-genome sequence of Listeria welshimeri reveals common steps in genome reduction with Listeria innocua as compared to Listeria monocytogenes." Hain T., Steinweg C., Kuenne C.T., Billion A., Ghai R., Chatterjee S.S., Domann E., Kaerst U., Goesmann A., Bekel T., Bartels D., Kaiser O., Meyer F., Puehler A., Weisshaar B., Wehland J., Liang C., Dandekar T. , Lampidis R., Kreft J., Goebel W., Chakraborty T. J. Bacteriol. 188:7405-7415(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35897 / DSM 20650 / SLCC5334.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM263198 Genomic DNA. Translation: CAK20697.1.
RefSeq	YP_849476.1. NC_008555.1.
3D structure databases
ProteinModelPortal	A0AI65.
ModBase	Search...
Protein-protein interaction databases
STRING	386043.lwe1279.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAK20697; CAK20697; lwe1279.
GeneID	4465450.
KEGG	lwe:lwe1279.
PATRIC	20329701. VBILisWel39304_1285.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0263.
HOGENOM	HOG000246368.
KO	K00931.
OMA	VYTGNPK.
ProtClustDB	PRK12314.
Enzyme and pathway databases
BioCyc	LWEL386043:GI5X-1301-MONOMER.
UniPathway	UPA00098; UER00359.
Family and domain databases
Gene3D	3.40.1160.10. 1 hit.
HAMAP	MF_00456. ProB.
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR019797. Glutamate_5-kinase_CS. [Graphical view]
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_LISW6

Accession

Primary (citable) accession number: A0AI65

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	November 28, 2006
Last modified:	May 29, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program