A0AI64 (PROA_LISW6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	lwe1278

Organism

Listeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334) [Complete proteome] [HAMAP]

Taxonomic identifier

386043 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_1000049961

Sequences

	Sequence		Length	Mass (Da)
	A0AI64 [UniParc]. Last modified November 28, 2006. Version 1. Checksum: A61437CB17D2C756	FASTA	415	45,776
	10 20 30 40 50 60 MTELIKKGIA AREASSFLAQ ATTKQKNTAL INLSNDLIAN TATLLKENEK DIIRAKEKKT 70 80 90 100 110 120 PDTMIDRLRL TEERIKEISE AVKQVVTLKD PIGEVTTMWK NEAELTIGKI RVPLGVIGII 130 140 150 160 170 180 YESRPNVTVD ASILCFKTGN AVILRGGSDA IDSNKALMNV IQESLEKSGF PRTSVQLIED 190 200 210 220 230 240 TSRETAREMM RLNRFLDVLI PRGGAKLIQT VLENATVPVI ETGTGNCHIY VDKAAEKQMA 250 260 270 280 290 300 IDILVNAKCS RPSVCNSAET LLIHESVAED FLPAMEMALK EYNVELRADK RAREILKDAK 310 320 330 340 350 360 AATESDWEEE FLDFILAIKV VDSVEEAINH INKYGTKHSE AIISNDYATG QAFHQKVDAA 370 380 390 400 410 AVYINASTRF TDGFAMGFGA EIGISTQKLH ARGPMGLTEL TSTKYIIFGD GQIRN

References

[1]

"Whole-genome sequence of Listeria welshimeri reveals common steps in genome reduction with Listeria innocua as compared to Listeria monocytogenes." Hain T., Steinweg C., Kuenne C.T., Billion A., Ghai R., Chatterjee S.S., Domann E., Kaerst U., Goesmann A., Bekel T., Bartels D., Kaiser O., Meyer F., Puehler A., Weisshaar B., Wehland J., Liang C., Dandekar T. , Lampidis R., Kreft J., Goebel W., Chakraborty T. J. Bacteriol. 188:7405-7415(2006) [PubMed: 16936040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35897 / DSM 20650 / SLCC5334.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM263198 Genomic DNA. Translation: CAK20696.1.
RefSeq	YP_849475.1. NC_008555.1.
3D structure databases
ProteinModelPortal	A0AI64.
ModBase	Search...
Protein-protein interaction databases
STRING	A0AI64.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4465449.
GenomeReviews	Gene locus lwe1278 in contig AM263198_GR.
KEGG	lwe:lwe1278.
NMPDR	fig|386043.6.peg.1238.
PATRIC	20329699. VBILisWel39304_1284.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0014.
HOGENOM	HBG318080.
OMA	QYPAACN.
PhylomeDB	A0AI64.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	LWEL386043:LWE1278-MONOMER.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
KO	K00147.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_LISW6

Accession

Primary (citable) accession number: A0AI64

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 28, 2006
Last modified:	December 14, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program