A0AFT8 (ISDG_LISW6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heme-degrading monooxygenase isdG
EC=1.14.99.3

Alternative name(s):
Heme oxygenase
Iron-regulated surface determinant isdG
Iron-responsive surface determinant isdG

Gene names

Name:	isdG
Ordered Locus Names:	lwe0452

Organism

Listeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334) [Complete proteome] [HAMAP]

Taxonomic identifier

386043 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	121 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows bacterial pathogens to use the host heme as an iron source. Catalyzes the oxidative degradation of the heme macrocyclic porphyrin ring in the presence of a suitable electron donor such as ascorbate or NADPH--cytochrome P450 reductase, with subsequent release of free iron By similarity. HAMAP MF_01272
Catalytic activity	Heme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O. HAMAP MF_01272
Subunit structure	Homodimer By similarity. HAMAP MF_01272
Subcellular location	Cytoplasm By similarity HAMAP MF_01272.
Sequence similarities	Belongs to the antibiotic biosynthesis monooxygenase family. Heme-degrading monooxygenase isdG subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	iron assimilation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	heme oxygenase (decyclizing) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 121	121	Heme-degrading monooxygenase isdG HAMAP MF_01272		PRO_1000067383
Sites
	Metal binding	6	1	Iron Potential
	Metal binding	84	1	Iron (heme axial ligand) Potential
	Site	74	1	Transition state stabilizer Potential

Sequences

	Sequence		Length	Mass (Da)
	A0AFT8 [UniParc]. Last modified November 28, 2006. Version 1. Checksum: 956031E4F8DA2D49	FASTA	121	13,845
	10 20 30 40 50 60 MIIVTNTIKV EKGAAEHVIR QFTGANGDGH PTKDIAEVEG FLGFELWHSK PEDKDYEEVV 70 80 90 100 110 120 VTSKWESEEA QRNWVKSDSF KKAHGRTKDT REQREDRKGI VGNEIARFEV VHVQNPVTIE K

References

[1]

"Whole-genome sequence of Listeria welshimeri reveals common steps in genome reduction with Listeria innocua as compared to Listeria monocytogenes." Hain T., Steinweg C., Kuenne C.T., Billion A., Ghai R., Chatterjee S.S., Domann E., Kaerst U., Goesmann A., Bekel T., Bartels D., Kaiser O., Meyer F., Puehler A., Weisshaar B., Wehland J., Liang C., Dandekar T. , Lampidis R., Kreft J., Goebel W., Chakraborty T. J. Bacteriol. 188:7405-7415(2006) [PubMed: 16936040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35897 / DSM 20650 / SLCC5334.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM263198 Genomic DNA. Translation: CAK19870.1.
RefSeq	YP_848653.1. NC_008555.1.
3D structure databases
ProteinModelPortal	A0AFT8.
ModBase	Search...
Protein-protein interaction databases
STRING	A0AFT8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4464193.
GenomeReviews	Gene locus lwe0452 in contig AM263198_GR.
KEGG	lwe:lwe0452.
NMPDR	fig|386043.6.peg.431.
PATRIC	20328029. VBILisWel39304_0455.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2329.
HOGENOM	HBG112566.
OMA	WTKSSAF.
ProtClustDB	PRK13316.
Enzyme and pathway databases
BioCyc	LWEL386043:LWE0452-MONOMER.
Family and domain databases
HAMAP	MF_01272. IsdG. [Tree]
InterPro	IPR007138. Antibiotic_mOase. IPR011008. Dimeric_a/b-barrel. IPR023953. Heme-degrad_mOase. [Graphical view]
KO	K07145.
Pfam	PF03992. ABM. 1 hit. [Graphical view]
SUPFAM	SSF54909. Dimer_A_B_barrel. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISDG_LISW6

Accession

Primary (citable) accession number: A0AFT8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 28, 2006
Last modified:	December 14, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program