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A0AEF6

- A0AEF6_RUMFL

UniProt

A0AEF6 - A0AEF6_RUMFL

Protein
Submitted name:

Cell-wall anchoring protein

Gene

scaE

Organism
Ruminococcus flavefaciens
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 17 (01 Oct 2014)
      Sequence version 1 (28 Nov 2006)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi165 – 1651CalciumImported
    Metal bindingi166 – 1661Calcium; via carbonyl oxygenImported
    Binding sitei185 – 1851Chloride 1; via amide nitrogen
    Metal bindingi200 – 2001Calcium; via carbonyl oxygenImported
    Binding sitei204 – 2041Chloride 2
    Metal bindingi218 – 2181Calcium; via carbonyl oxygenImported
    Metal bindingi220 – 2201CalciumImported

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. metal ion binding Source: UniProtKB-KW

    Keywords - Ligandi

    CalciumImported, Metal-bindingImported

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Cell-wall anchoring proteinImported
    Gene namesi
    Name:scaEImported
    OrganismiRuminococcus flavefaciensImported
    Taxonomic identifieri1265 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminococcus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929 PotentialImportedAdd
    BLAST
    Chaini30 – 330301 PotentialImportedPRO_5000147936Add
    BLAST

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4IU2X-ray2.00A29-230[»]
    4IU3X-ray1.97A29-230[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Keywords - Domaini

    SignalImported

    Family and domain databases

    InterProiIPR008965. Carb-bd_dom.
    [Graphical view]
    SUPFAMiSSF49384. SSF49384. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A0AEF6-1 [UniParc]FASTAAdd to Basket

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    MKKNTLKKAI SAVAVSALAL TASSVSAFAL TDRGMTYDLD PKDGSSAATK    50
    PVLEVTKKVF DTAADAAGQT VTVEFKVSGA EGKYATTGYH IYWDERLEVV 100
    ATKTGAYAKK GAALEDSSLA KAENNGNGVF VASGADDDFG ADGVMWTVEL 150
    KVPADAKAGD VYPIDVAYQW DPSKGDLFTD NKDSAQGKLM QAYFFTQGIK 200
    SSSNPSTDEY LVKANATYAD GYIAIKAGEP ETTTTTTTTT TTTTTTTTTT 250
    TTTTSTSSTT TTTGSTTSAN AESTTGAAQS TGAQTTTAKS NSSDNKKTDS 300
    PKTGVTGVGV AVAGLAVAVG TAFALKKKED 330
    Length:330
    Mass (Da):33,924
    Last modified:November 28, 2006 - v1
    Checksum:iE0634623E4A3F577
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM262974 Genomic DNA. Translation: CAK18898.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM262974 Genomic DNA. Translation: CAK18898.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4IU2 X-ray 2.00 A 29-230 [» ]
    4IU3 X-ray 1.97 A 29-230 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR008965. Carb-bd_dom.
    [Graphical view ]
    SUPFAMi SSF49384. SSF49384. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Conservation and divergence in cellulosome architecture between two strains of Ruminococcus flavefaciens."
      Sadanari J., Borovok I., Rincon M.T., Flint H.J., Antonopoulos D.A., Berg M.E., White B.A., Bayer E.A., Lamed R.
      J. Bacteriol. 188:7971-7976(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: FD-1Imported.
    2. "Atypical cohesin-dockerin complex responsible for cell surface attachment of cellulosomal components: binding fidelity, promiscuity, and structural buttresses."
      Salama-Alber O., Jobby M.K., Chitayat S., Smith S.P., White B.A., Shimon L.J., Lamed R., Frolow F., Bayer E.A.
      J. Biol. Chem. 288:16827-16838(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.97 ANGSTROMS) OF 29-230 IN COMPLEX WITH CALCIUM.

    Entry informationi

    Entry nameiA0AEF6_RUMFL
    AccessioniPrimary (citable) accession number: A0AEF6
    Entry historyi
    Integrated into UniProtKB/TrEMBL: November 28, 2006
    Last sequence update: November 28, 2006
    Last modified: October 1, 2014
    This is version 17 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3