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A0AEF6

- A0AEF6_RUMFL

UniProt

A0AEF6 - A0AEF6_RUMFL

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Protein
Submitted name:

Cell-wall anchoring protein

Gene
scaE
Organism
Ruminococcus flavefaciens
Status
Unreviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi165 – 1651CalciumImported
Metal bindingi166 – 1661Calcium; via carbonyl oxygenImported
Binding sitei185 – 1851Chloride 1; via amide nitrogen
Metal bindingi200 – 2001Calcium; via carbonyl oxygenImported
Binding sitei204 – 2041Chloride 2
Metal bindingi218 – 2181Calcium; via carbonyl oxygenImported
Metal bindingi220 – 2201CalciumImported

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Ligandi

CalciumImported, Metal-bindingImported

Names & Taxonomyi

Protein namesi
Submitted name:
Cell-wall anchoring proteinImported
Gene namesi
Name:scaEImported
OrganismiRuminococcus flavefaciensImported
Taxonomic identifieri1265 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929 PredictedImportedAdd
BLAST
Chaini30 – 330301 PredictedImportedPRO_5000147936Add
BLAST

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4IU2X-ray2.00A29-230[»]
4IU3X-ray1.97A29-230[»]

Family & Domainsi

Keywords - Domaini

SignalImported

Family and domain databases

InterProiIPR008965. Carb-bd_dom.
[Graphical view]
SUPFAMiSSF49384. SSF49384. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A0AEF6-1 [UniParc]FASTAAdd to Basket

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MKKNTLKKAI SAVAVSALAL TASSVSAFAL TDRGMTYDLD PKDGSSAATK    50
PVLEVTKKVF DTAADAAGQT VTVEFKVSGA EGKYATTGYH IYWDERLEVV 100
ATKTGAYAKK GAALEDSSLA KAENNGNGVF VASGADDDFG ADGVMWTVEL 150
KVPADAKAGD VYPIDVAYQW DPSKGDLFTD NKDSAQGKLM QAYFFTQGIK 200
SSSNPSTDEY LVKANATYAD GYIAIKAGEP ETTTTTTTTT TTTTTTTTTT 250
TTTTSTSSTT TTTGSTTSAN AESTTGAAQS TGAQTTTAKS NSSDNKKTDS 300
PKTGVTGVGV AVAGLAVAVG TAFALKKKED 330
Length:330
Mass (Da):33,924
Last modified:November 28, 2006 - v1
Checksum:iE0634623E4A3F577
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM262974 Genomic DNA. Translation: CAK18898.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM262974 Genomic DNA. Translation: CAK18898.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4IU2 X-ray 2.00 A 29-230 [» ]
4IU3 X-ray 1.97 A 29-230 [» ]
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR008965. Carb-bd_dom.
[Graphical view ]
SUPFAMi SSF49384. SSF49384. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Conservation and divergence in cellulosome architecture between two strains of Ruminococcus flavefaciens."
    Sadanari J., Borovok I., Rincon M.T., Flint H.J., Antonopoulos D.A., Berg M.E., White B.A., Bayer E.A., Lamed R.
    J. Bacteriol. 188:7971-7976(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: FD-1Imported.
  2. "Atypical cohesin-dockerin complex responsible for cell surface attachment of cellulosomal components: binding fidelity, promiscuity, and structural buttresses."
    Salama-Alber O., Jobby M.K., Chitayat S., Smith S.P., White B.A., Shimon L.J., Lamed R., Frolow F., Bayer E.A.
    J. Biol. Chem. 288:16827-16838(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.97 ANGSTROMS) OF 29-230 IN COMPLEX WITH CALCIUM.

Entry informationi

Entry nameiA0AEF6_RUMFL
AccessioniPrimary (citable) accession number: A0AEF6
Entry historyi
Integrated into UniProtKB/TrEMBL: November 28, 2006
Last sequence update: November 28, 2006
Last modified: September 3, 2014
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3