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Protein

Protein LSM14 homolog A-A

Gene

lsm14a-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding component of messenger ribonucleoprotein complexes (mRNPs), storage particles that mask maternal mRNAs from the translational apparatus during oocyte maturation. Acts as a repressor of mRNA translation.1 Publication

GO - Molecular functioni

  • DEAD/H-box RNA helicase binding Source: UniProtKB
  • RNA binding Source: UniProtKB

GO - Biological processi

  • multicellular organism development Source: UniProtKB-KW
  • negative regulation of translation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor, Ribonucleoprotein

Keywords - Biological processi

Translation regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein LSM14 homolog A-A
Alternative name(s):
RNA-associated protein 55A-A
Short name:
RAP55A-A
Short name:
xRAP552 Publications
Short name:
xRAP55A2 Publications
Gene namesi
Name:lsm14a-a
Synonyms:lsm14a, rap55a-a
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-5955717. lsm14a.

Subcellular locationi

  • Cytoplasm 1 Publication

  • Note: Localizes to cytoplasmic particles in stage VI oocytes and eggs.1 Publication

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • intracellular ribonucleoprotein complex Source: UniProtKB
  • protein complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 471471Protein LSM14 homolog A-APRO_0000391378Add
BLAST

Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiA0A8M2.

Expressioni

Developmental stagei

Expressed both maternally and zygotically. Expression increases during oocyte growth and continues at a constant level until the tailbud stage (at protein level).3 Publications

Interactioni

Subunit structurei

Component of a ribonucleoprotein (RNP) complex, at least composed of lsm14a/rap55a, ybx2/frgy2, ddx6/Xp54 and eif4enif1/4E-T. Also forms a complex with prmt1 independently of ybx2/frgy2. Interacts with ddx6/Xp54 but does not appear to directly bind ybx2/frgy2. Different translationally-repressed mRNP complexes probably exist that contain either lsm14a/rap55a or lsm14b/rap55b depending on the developmental stage.1 Publication

GO - Molecular functioni

  • DEAD/H-box RNA helicase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi104191. 1 interaction.
IntActiA0A8M2. 3 interactions.
MINTiMINT-7297356.

Structurei

3D structure databases

ProteinModelPortaliA0A8M2.
SMRiA0A8M2. Positions 6-83.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini288 – 32437DFDFPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi363 – 37917FFD boxAdd
BLAST
Motifi382 – 40221TFG boxAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi353 – 3564Poly-GluSequence analysis
Compositional biasi407 – 45549Gly-richSequence analysisAdd
BLAST

Domaini

The RGG repeats are required for interaction with ddx6/Xp54 and accumulation in ribonucleoprotein complexes.1 Publication

Sequence similaritiesi

Belongs to the LSM14 family.Sequence analysis
Contains 1 DFDF domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG054326.
KOiK18749.

Family and domain databases

InterProiIPR025762. DFDF.
IPR019050. FDF_dom.
IPR025761. FFD_box.
IPR025609. Lsm14_N.
IPR010920. LSM_dom.
IPR025768. TFG_box.
[Graphical view]
PfamiPF09532. FDF. 1 hit.
PF12701. LSM14. 1 hit.
[Graphical view]
SMARTiSM01199. FDF. 1 hit.
SM01271. LSM14. 1 hit.
[Graphical view]
SUPFAMiSSF50182. SSF50182. 1 hit.
PROSITEiPS51512. DFDF. 1 hit.
PS51513. FFD. 1 hit.
PS51536. TFG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0A8M2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGGTPYIGS KISLISKAEI RYEGILYTID TENSTVALAK VRSFGTEDRP
60 70 80 90 100
TDRPIPPRDE IFEYIIFRGS DIKDLTVCEP PKPQCSLPQD PAIVQSSLGS
110 120 130 140 150
SSASSFQSVS SYGPFGRMPA YSQFNTGPLV GPQFGAVGVG SSLTSFGAET
160 170 180 190 200
TSSTSLPPSS AVGTSFTQEA RTLKTQSSQG QSSSPLDSLR KSPNIEQAVQ
210 220 230 240 250
TAAAPHAPST ATVGRRSPVL SRPVPSSIQK TAESPEQRKG ELHKMQRPDI
260 270 280 290 300
DQLKNDKNDP SKRQPVLSAL QPRRGRGGNR GGRGRFGVRR DGPMKFEKDF
310 320 330 340 350
DFESANAQFN KEEIDREFHN KLKIKDDKPE KPVNGEDKTD SVVDTQNSEG
360 370 380 390 400
NAEEEEVLAG GVCYYDKTKS FFDNISCDDN RDRRQTWAEE RRINVETFGL
410 420 430 440 450
PLRSNRGRGG FRGRGGGMGF RGGRGRGGER RGAPGGGGFG PARGFRGGFR
460 470
GGRGGREFAD YEYRKDNKVA A
Length:471
Mass (Da):51,168
Last modified:November 28, 2006 - v1
Checksum:i67A85C7E2FE93398
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti352 – 3521A → V in AAH80031 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB257699 mRNA. Translation: BAF36055.1.
BC080031 mRNA. Translation: AAH80031.1.
RefSeqiNP_001087507.1. NM_001094038.1.
UniGeneiXl.47199.

Genome annotation databases

GeneIDi447331.
KEGGixla:447331.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB257699 mRNA. Translation: BAF36055.1.
BC080031 mRNA. Translation: AAH80031.1.
RefSeqiNP_001087507.1. NM_001094038.1.
UniGeneiXl.47199.

3D structure databases

ProteinModelPortaliA0A8M2.
SMRiA0A8M2. Positions 6-83.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi104191. 1 interaction.
IntActiA0A8M2. 3 interactions.
MINTiMINT-7297356.

Proteomic databases

PRIDEiA0A8M2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi447331.
KEGGixla:447331.

Organism-specific databases

CTDi447331.
XenbaseiXB-GENE-5955717. lsm14a.

Phylogenomic databases

HOVERGENiHBG054326.
KOiK18749.

Family and domain databases

InterProiIPR025762. DFDF.
IPR019050. FDF_dom.
IPR025761. FFD_box.
IPR025609. Lsm14_N.
IPR010920. LSM_dom.
IPR025768. TFG_box.
[Graphical view]
PfamiPF09532. FDF. 1 hit.
PF12701. LSM14. 1 hit.
[Graphical view]
SMARTiSM01199. FDF. 1 hit.
SM01271. LSM14. 1 hit.
[Graphical view]
SUPFAMiSSF50182. SSF50182. 1 hit.
PROSITEiPS51512. DFDF. 1 hit.
PS51513. FFD. 1 hit.
PS51536. TFG. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "RAP55, a cytoplasmic mRNP component, represses translation in Xenopus oocytes."
    Tanaka K.J., Ogawa K., Takagi M., Imamoto N., Matsumoto K., Tsujimoto M.
    J. Biol. Chem. 281:40096-40106(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, IDENTIFICATION IN A RIBONUCLEOPROTEIN COMPLEX WITH YBX2; DDX6 AND EIF4ENIF1, IDENTIFICATION IN A COMPLEX WITH PRMT1; INTERACTION WITH DDX6, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, DOMAIN, PHOSPHORYLATION.
    Tissue: Oocyte1 Publication.
  2. NIH - Xenopus Gene Collection (XGC) project
    Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Tail budImported.
  3. "Identification of a novel mRNA-associated protein in oocytes of Pleurodeles waltl and Xenopus laevis."
    Lieb B., Carl M., Hock R., Gebauer D., Scheer U.
    Exp. Cell Res. 245:272-281(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  4. "CPEB interacts with an ovary-specific eIF4E and 4E-T in early Xenopus oocytes."
    Minshall N., Reiter M.H., Weil D., Standart N.
    J. Biol. Chem. 282:37389-37401(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  5. "RAP55: insights into an evolutionarily conserved protein family."
    Marnef A., Sommerville J., Ladomery M.R.
    Int. J. Biochem. Cell Biol. 41:977-981(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiL14AA_XENLA
AccessioniPrimary (citable) accession number: A0A8M2
Secondary accession number(s): Q68F15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 9, 2010
Last sequence update: November 28, 2006
Last modified: May 11, 2016
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

In contrast to PubMed:17074753, PubMed:9851867 show a strictly maternal expression that decreases progressively during oocyte maturation and early cleavage stages. This is more similar to the expression pattern of lsm14b/rap55b.Curated

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.