Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha-1,3-galactosyltransferase 2

Gene

A3galt2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Synthesizes the galactose-alpha(1,3)-galactose group on the glycosphingolipid isoglobotrihexosylceramide or isogloboside 3 (iGb3) by catalyzing the transfer of galactose from UDP-Galactose to its acceptor molecule Gal-beta-1,4-Glc-ceramide. Can also catalyze the addition of galactose to iGb3 itself to form polygalactose structures. Synthesis of iGb3 is the initial step in the formation of the isoglobo-series glycolipid pathway and is the precursor to isogloboside 4 (iGb4) and isoForssman glycolipids. Can glycosylate only lipids and not proteins and is solely responsible for initiating the synthesis of isoglobo-series glycosphingolipids.3 Publications

Catalytic activityi

UDP-alpha-D-galactose + beta-D-galactosyl-(1->4)-beta-N-acetyl-D-glucosaminyl-R = UDP + alpha-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-N-acetylglucosaminyl-R.1 Publication

Cofactori

Mn2+By similarityNote: Binds 1 Mn2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi198ManganeseBy similarity1
Metal bindingi200ManganeseBy similarity1
Active sitei290NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • glycosphingolipid biosynthetic process Source: UniProtKB
  • lipid glycosylation Source: UniProtKB

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandManganese, Metal-binding

Protein family/group databases

CAZyiGT6 Glycosyltransferase Family 6

Chemistry databases

SwissLipidsiSLP:000000794

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3-galactosyltransferase 2By similarity (EC:2.4.1.871 Publication)
Alternative name(s):
Isoglobotriaosylceramide synthase
Short name:
iGb3 synthase1 Publication
Short name:
iGb3S1 Publication
Gene namesi
Name:A3galt2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi727913 A3galt2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11CytoplasmicSequence analysisAdd BLAST11
Transmembranei12 – 31Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST20
Topological domaini32 – 339LumenalSequence analysisAdd BLAST308

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi187L → P: Significant loss of activity. 1 Publication1
Mutagenesisi198 – 200DVD → AVA: Catalytically inactive. 1 Publication3
Mutagenesisi252Y → N: Complete loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003148721 – 339Alpha-1,3-galactosyltransferase 2Add BLAST339

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi57N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi99N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiA0A4Z3
PRIDEiA0A4Z3

Expressioni

Tissue specificityi

Dorsal root ganglia neurons (at protein level). High levels in spleen, thymus and skeletal muscle, intermediate levels in lung, uterus, pituitary and heart, low levels in brain, and undetected in adrenal gland and liver.3 Publications

Gene expression databases

BgeeiENSRNOG00000005935
GenevisibleiA0A4Z3 RN

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000007851

Structurei

3D structure databases

ProteinModelPortaliA0A4Z3
SMRiA0A4Z3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni107 – 112Substrate bindingBy similarity6
Regioni198 – 200Substrate bindingBy similarity3
Regioni220 – 223Substrate bindingBy similarity4
Regioni332 – 338Substrate bindingBy similarity7

Domaini

The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.

Sequence similaritiesi

Belongs to the glycosyltransferase 6 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHE7 Eukaryota
ENOG4111IPQ LUCA
GeneTreeiENSGT00400000022032
HOGENOMiHOG000234339
HOVERGENiHBG003563
InParanoidiA0A4Z3
KOiK20736
OMAiGAPIIWD
OrthoDBiEOG091G0A87
PhylomeDBiA0A4Z3
TreeFamiTF330991

Family and domain databases

Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR005076 Glyco_trans_6
IPR029044 Nucleotide-diphossugar_trans
PANTHERiPTHR10462 PTHR10462, 1 hit
PfamiView protein in Pfam
PF03414 Glyco_transf_6, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit

Sequencei

Sequence statusi: Complete.

A0A4Z3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALEGLRAKK RLLWRLFLSA FGLLGLYHYW FKIFRLFEVF IPMGICPMAI
60 70 80 90 100
MPLLKDNFTG VLRHWARPEV LTCTSWGAPI IWDETFDPHV AEREARRQNL
110 120 130 140 150
TIGLTVFAVG RYLEKYLEHF LVSAEQYFMV GQNVVYYVFT DRPEAVPHVA
160 170 180 190 200
LGQGRLLRVK PVRREKRWQD VSMARMLTLH EALGGQLGRE ADYVFCLDVD
210 220 230 240 250
QYFSGNFGPE VLADLVAQLH AWHFRWPRWM LPYERDKRSA AALSLSEGDF
260 270 280 290 300
YYHAAVFGGS VAALLKLTAH CATGQQLDRE HGIEARWHDE SHLNKFFWLS
310 320 330
KPTKLLSPEF CWAEEIGWRP EIHHPRLIWA PKEYALVRT
Length:339
Mass (Da):39,548
Last modified:November 28, 2006 - v1
Checksum:i171A191001F0C6CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF248543 mRNA Translation: AAF82757.1
BC061714 mRNA Translation: AAH61714.1
RefSeqiNP_612533.1, NM_138524.2
UniGeneiRn.37737

Genome annotation databases

EnsembliENSRNOT00000007851; ENSRNOP00000007851; ENSRNOG00000005935
GeneIDi171553
KEGGirno:171553

Similar proteinsi

Entry informationi

Entry nameiA3LT2_RAT
AccessioniPrimary (citable) accession number: A0A4Z3
Secondary accession number(s): A2JQW1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 28, 2006
Last modified: May 23, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health