Reviewed, UniProtKB/Swiss-Prot A0A4Z3 (A3LT2_RAT)

Names and origin

Protein names

Recommended name:
    Alpha 1,3-galactosyltransferase 2
      Short name=A3galt2
    EC=2.4.1.87
Alternative name(s):
    Isoglobotriaosylceramide synthase
    iGb3 synthase
      Short name=iGb3S

Gene names

Name:

A3galt2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Transfer of galactose from UDP-galactose to an acceptor molecule (R). Synthesizes the galactose-alpha(1,3)-galactose group, which is the most common carbohydrate containing terminal alpha-galactose. Preferentially glycosylates lipids.
Catalytic activity	UDP-galactose + beta-D-galactosyl-(1->4)-beta-N-acetyl-D-glucosaminyl-R = UDP + alpha-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-N-acetylglucosaminyl-R.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus Potential.
Domain	The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.
Sequence similarities	Belongs to the glycosyltransferase 6 family.

Ontologies

Keywords
Cellular component	Golgi apparatus
Domain	Signal
Ligand	Manganese Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro glycosphingolipid biosynthetic process Inferred from direct assay. Source: RGD
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular function	N-acetyllactosaminide 3-alpha-galactosyltransferase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 339	314	Alpha 1,3-galactosyltransferase 2		PRO_0000314872
Sites
	Metal binding	198	1	Manganese By similarity
	Metal binding	200	1	Manganese By similarity
Amino acid modifications
	Glycosylation	57	1	N-linked (GlcNAc...) Potential
	Glycosylation	99	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A0A4Z3-1 [UniParc]. Last modified November 28, 2006. Version 1. Checksum: 171A191001F0C6CF	FASTA	339	39,548
	10 20 30 40 50 60 MALEGLRAKK RLLWRLFLSA FGLLGLYHYW FKIFRLFEVF IPMGICPMAI MPLLKDNFTG 70 80 90 100 110 120 VLRHWARPEV LTCTSWGAPI IWDETFDPHV AEREARRQNL TIGLTVFAVG RYLEKYLEHF 130 140 150 160 170 180 LVSAEQYFMV GQNVVYYVFT DRPEAVPHVA LGQGRLLRVK PVRREKRWQD VSMARMLTLH 190 200 210 220 230 240 EALGGQLGRE ADYVFCLDVD QYFSGNFGPE VLADLVAQLH AWHFRWPRWM LPYERDKRSA 250 260 270 280 290 300 AALSLSEGDF YYHAAVFGGS VAALLKLTAH CATGQQLDRE HGIEARWHDE SHLNKFFWLS 310 320 330 KPTKLLSPEF CWAEEIGWRP EIHHPRLIWA PKEYALVRT

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC061714 mRNA. Translation: AAH61714.1.
IPI	IPI00197419.
RefSeq	NP_612533.1.
UniGene	Rn.37737
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000007851; ENSRNOP00000007851; ENSRNOG00000005935; Rattus norvegicus. [Genome view]
GeneID	171553.
KEGG	rno:171553.
Organism-specific databases
CTD	171553.
RGD	727913. A3galt2.
Phylogenomic databases
HOVERGEN	A0A4Z3.
Enzyme and pathway databases
BRENDA	2.4.1.87. 248.
Gene expression databases
Genevestigator	A0A4Z3.
Family and domain databases
InterPro	IPR005076. Glyco_trans_6. [Graphical view]
PANTHER	PTHR10462. Glyco_trans_6. 1 hit.
Pfam	PF03414. Glyco_transf_6. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	622541.

Entry information

Entry name

A3LT2_RAT

Accession

Primary (citable) accession number: A0A4Z3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 28, 2006
Last modified:	October 13, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)