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Protein

DNA topoisomerase 3

Gene

topB

Organism
Vibrio shilonii
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi8Magnesium 1; catalyticUniRule annotation1
Metal bindingi104Magnesium 1; catalyticUniRule annotation1
Metal bindingi104Magnesium 2UniRule annotation1
Metal bindingi106Magnesium 2UniRule annotation1
Active sitei332O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotation, Isomerase, TopoisomeraseUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IIIUniRule annotation
Gene namesi
Name:topBUniRule annotation
ORF Names:BSZ05_01340Imported
OrganismiVibrio shiloniiImported
Taxonomic identifieri62153 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000197092 Componenti: Chromosome 1

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei62Interaction with DNAUniRule annotation1
Sitei171Interaction with DNAUniRule annotation1
Sitei179Interaction with DNAUniRule annotation1
Sitei334Interaction with DNAUniRule annotation1

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 135ToprimInterPro annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni195 – 200Interaction with DNAUniRule annotation6

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation

Phylogenomic databases

KOiK03169.

Family and domain databases

CDDicd00186. TOP1Ac. 1 hit.
cd03362. TOPRIM_TopoIA_TopoIII. 1 hit.
Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
HAMAPiMF_00953. Topoisom_3_prok. 1 hit.
InterProiView protein in InterPro
IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005738. TopoIII.
IPR006171. TOPRIM_domain.
IPR034144. TOPRIM_TopoIII.
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiView protein in Pfam
PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PRINTSiPR00417. PRTPISMRASEI.
SMARTiView protein in SMART
SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01056. topB. 1 hit.
PROSITEiView protein in PROSITE
PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A2C9P7C4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRLFIAEKP SLGRAIAAAL PNPQKKGDGY IQCGNGDVVT WCIGHLLEQV
60 70 80 90 100
EPDAYDERYK KWNLNDLPIV PNQWQLRPRK SASKQLTAVK KLLKTATSIV
110 120 130 140 150
NAGDPDREGQ LLVDEVIDYC KVPKAKKDTA QRLLISDLNL PAVKKALNSM
160 170 180 190 200
RMNQEFIPLS VSALARSRAD WLYGMNMSRA YTLLGQKAGY QGVLSVGRVQ
210 220 230 240 250
TPVLGLVVRR DEEIENFVPK DYYTLHALIP YQDSHAAFDI RAKWKPSEAC
260 270 280 290 300
QPWQDEEGRV LNKKLVENVA SRIQGQPAKV TKSEHKSTKQ NAPLPYSLSA
310 320 330 340 350
LQIDAAKRFN MSAQQVLDVC QALYEKHKLI TYPRSDNRYL PKDHFYQAND
360 370 380 390 400
VLSAIKNNSR EHAQAVDDAD SSRKSKAWND SKVDAHHAII PTPKKTSSVL
410 420 430 440 450
SGFEEKIYGL IARQYLMQFY PAAVYAEAML EFDIQGGCFI AKGKRLQQPG
460 470 480 490 500
WRVLLGNSAN EQEDGVDAVP PLEVGTELIC HEGVIAEKKT EPPRHFTEAT
510 520 530 540 550
LLQAMTGISR FVEDKSLKKI LRDNDGIGTE ATRAGILDTL FKRKLLNRQG
560 570 580 590 600
KSVISTSAGR GLIHALPDES TYPDMTAHWE FQLQGMAEKT QSYTPFMAEL
610 620 630 640 650
NNRLEQLMQQ VKSGPVPDSL KNLPKVERPA FKKRRFSKGK KAPSKSKNRS
Length:650
Mass (Da):72,922
Last modified:December 20, 2017 - v1
Checksum:i712D1A010B53F685
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP018308 Genomic DNA. Translation: ASI88565.1.

Genome annotation databases

KEGGivsh:BSZ05_01340.

Similar proteinsi

Entry informationi

Entry nameiA0A2C9P7C4_9VIBR
AccessioniPrimary (citable) accession number: A0A2C9P7C4
Entry historyiIntegrated into UniProtKB/TrEMBL: December 20, 2017
Last sequence update: December 20, 2017
Last modified: February 28, 2018
This is version 3 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteomeImported