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Protein

Pup--protein ligase

Gene

pafA

Organism
Amycolatopsis sulphurea
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves the side-chain carboxylate of the C-terminal glutamate of Pup and the side-chain amino group of a substrate lysine.UniRule annotation

Miscellaneous

The reaction mechanism probably proceeds via the activation of Pup by phosphorylation of its C-terminal glutamate, which is then subject to nucleophilic attack by the substrate lysine, resulting in an isopeptide bond and the release of phosphate as a good leaving group.UniRule annotation

Catalytic activityi

ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein]-L-lysine = ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein]-L-lysine.UniRule annotationSAAS annotation

Pathwayi: proteasomal Pup-dependent pathway

This protein is involved in the pathway proteasomal Pup-dependent pathway, which is part of Protein degradation.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway proteasomal Pup-dependent pathway and in Protein degradation.

Pathwayi: protein pupylation

This protein is involved in the pathway protein pupylation, which is part of Protein modification.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway protein pupylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi9MagnesiumUniRule annotation1
Binding sitei53ATPUniRule annotation1
Metal bindingi55MagnesiumUniRule annotation1
Active sitei57Proton acceptorUniRule annotation1
Metal bindingi63MagnesiumUniRule annotation1
Binding sitei66ATP; via carbonyl oxygenUniRule annotation1
Binding sitei419ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationSAAS annotation
Biological processUbl conjugation pathwayUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00997.
UPA00998.

Names & Taxonomyi

Protein namesi
Recommended name:
Pup--protein ligaseUniRule annotation (EC:6.3.1.19UniRule annotation)
Alternative name(s):
Proteasome accessory factor AUniRule annotation
Pup-conjugating enzymeUniRule annotation
Gene namesi
Name:pafAUniRule annotation
ORF Names:ATK36_1025Imported
OrganismiAmycolatopsis sulphureaImported
Taxonomic identifieri76022 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaeAmycolatopsis

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

ProteasomeImported

Family & Domainsi

Sequence similaritiesi

Belongs to the Pup ligase/Pup deamidase family. Pup-conjugating enzyme subfamily.UniRule annotationSAAS annotation

Family and domain databases

HAMAPiMF_02111. Pup_ligase. 1 hit.
InterProiView protein in InterPro
IPR022279. Pup_ligase.
IPR004347. Pup_ligase/deamidase.
PANTHERiPTHR42307. PTHR42307. 1 hit.
PfamiView protein in Pfam
PF03136. Pup_ligase. 1 hit.
PIRSFiPIRSF018077. UCP018077. 1 hit.
TIGRFAMsiTIGR03686. pupylate_PafA. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A2A9G3H3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQRRIFGIET EFGVTCTFHG QRRLSPDEVA RYLFRRVVSW GRSSNVFLSN
60 70 80 90 100
GSRLYLDVGS HPEYATAECD DLTQLVTHDK AGERILEDLL TDAERRLADE
110 120 130 140 150
GIGGDIFLFK NNTDSAGNSY GCHENYLVTR AGEFSRIADV LLPFLVTRQL
160 170 180 190 200
ICGAGKVLQT PRGAVYCLSQ RAEHIWEGVS SATTRSRPII NTRDEPHADA
210 220 230 240 250
ERYRRLHVIV GDSNMAEPTT MLKIGSANLV LEMIEAGVQF RDFTLDNPIR
260 270 280 290 300
AIREISHDLT GRRQVRLAGG REASALDIQR EYHARAVAHL KEHGSTPANE
310 320 330 340 350
RVVELWGRAL EAVELQDFSK IDTEIDWAIK HRLVERYRAK HDLDLSSPRV
360 370 380 390 400
AQIDLAYHDI RRGRGVFDLL QRKGLVQRVT DDGEIEVAKD TPPQTTRAKL
410 420 430 440 450
RGDFIAAAQA AGRDFTVDWV HLKLNDQAQR TVLCKDPFRS VDERVERLIS

SL
Length:452
Mass (Da):51,079
Last modified:December 20, 2017 - v1
Checksum:iC1CF45B6E848F19E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
PDJK01000001 Genomic DNA. Translation: PFG57441.1.

Similar proteinsi

Entry informationi

Entry nameiA0A2A9G3H3_9PSEU
AccessioniPrimary (citable) accession number: A0A2A9G3H3
Entry historyiIntegrated into UniProtKB/TrEMBL: December 20, 2017
Last sequence update: December 20, 2017
Last modified: February 28, 2018
This is version 3 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported