Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pasteurella multocida
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei562Aminoacyl-adenylateUniRule annotation1
Binding sitei606ATPUniRule annotation1
Metal bindingi899ZincUniRule annotation1
Metal bindingi902ZincUniRule annotation1
Metal bindingi915ZincUniRule annotation1
Metal bindingi918ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetaseUniRule annotationSAAS annotation, Ligase
Biological processProtein biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Metal-bindingUniRule annotation, Nucleotide-binding, ZincUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
ORF Names:CLD33_02240Imported
OrganismiPasteurella multocidaImported
Taxonomic identifieri747 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 641tRNA-synt_1InterPro annotationAdd BLAST613
Domaini686 – 842Anticodon_1InterPro annotationAdd BLAST157

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi59 – 69"HIGH" regionUniRule annotationAdd BLAST11
Motifi603 – 607"KMSKS" regionUniRule annotation5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
PfamiView protein in Pfam
PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A290LL04-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVDYKNTLN LPETGFPMRG DLAKREPNML KSWYEKDLYQ KIRQASKGKK
60 70 80 90 100
SFILHDGPPY ANGTIHIGHA VNKILKDIIV KSKTALGYDS PYIPGWDCHG
110 120 130 140 150
LPIELKVEGL VGKPNQNISA AQFREACRQY AAEQVEGQKK DFIRLGVLGD
160 170 180 190 200
WDNPYLTMNY HTEANIIRAF GKAVENGHLY KGSKPVHWCL DCASSLAEAE
210 220 230 240 250
VEYEDKVSPS IYVRFSAVDS DAVLAKFNAT DKGTGNISAV IWTTTPWTIP
260 270 280 290 300
SNRAIAIHEN LDYQLVQFND ERVILAKDLV EEVAKAAGVE QVIILGESKG
310 320 330 340 350
KDLEWLRFQH PFYDFSVPFI LGDHVTTDGG TGLVHTAPDH GHDDYIIAQK
360 370 380 390 400
NGIEMAGLIG NDGLFKADVP FFAGKGVFES NDLVVAKLQE VGAMLKFSKI
410 420 430 440 450
KHSYPHCWRH KTPIIFRATP QWFIGMEKQG LRQQALSEIK KVRWIPDWGQ
460 470 480 490 500
ARIEKMVENR PDWCISRQRT WGVPVALFIH KETEELHPRT VELVEEVAKR
510 520 530 540 550
VEQKGIQAWW DLDTAELLGA DADNYIKVPD TLDVWFDSGS TYYSVVKDRP
560 570 580 590 600
EFNGQEADMY LEGSDQHRGW FMSSLMLSTA TDNKAPYKQV LTHGFTVDGQ
610 620 630 640 650
GRKMSKSIGN IVTPQEVMDK FGGDILRLWV ASTDYTGEIS VSDEILKRAA
660 670 680 690 700
DAYRRIRNTA RFLLANLNGF DPKRDLVKPE EMMVLDRWAV DCAYQAQNEI
710 720 730 740 750
KDAYDNYQFH AVIQRLMKFC SIEMGSFYLD IIKDRQYTTK ADSLARRSCQ
760 770 780 790 800
TALWHIAEAL VRWIAPVLSF TADEIWQYIP GERGEFVFTE EFYNGLFALD
810 820 830 840 850
ANEQMNDAYW QQVITLRNEV NRVLEQARND KIIGAALEAE LTIYANDTYA
860 870 880 890 900
PLLAKLQNEL RFVLLTSKAE VKPLADADVA EGEVKGFAVK VVRSANHKCP
910 920 930
RCWHYSDSKD AESLCSRCDE NVNGQGEVRQ FA
Length:932
Mass (Da):105,653
Last modified:December 20, 2017 - v1
Checksum:i46C97C38CDDB1E0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP023304 Genomic DNA. Translation: ATC20933.1.

Similar proteinsi

Entry informationi

Entry nameiA0A290LL04_PASMD
AccessioniPrimary (citable) accession number: A0A290LL04
Entry historyiIntegrated into UniProtKB/TrEMBL: December 20, 2017
Last sequence update: December 20, 2017
Last modified: March 28, 2018
This is version 4 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)