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Protein

Thymidylate synthase

Gene

thyA

Organism
Polaromonas sp. 24-62-144
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotationSAAS annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei31dUMPUniRule annotation1
Binding sitei615,10-methylenetetrahydrofolateUniRule annotation1
Active sitei156PROSITE-ProRule annotation1
Active sitei156NucleophileUniRule annotation1
Binding sitei1865,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei194dUMPUniRule annotation1
Binding sitei2805,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi136 – 137dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi183 – 186dUMPUniRule annotation4
Nucleotide bindingi224 – 226dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferaseUniRule annotationSAAS annotation, Transferase
Biological processNucleotide biosynthesisUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotationSAAS annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
ORF Names:B7Y03_03755Imported
OrganismiPolaromonas sp. 24-62-144Imported
Taxonomic identifieri1970414 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaePolaromonas

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 281Thymidylat_syntInterPro annotationAdd BLAST269

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiView protein in InterPro
IPR023451. Thymidate_synth/dCMP_Mease.
IPR036926. Thymidate_synth/dCMP_Mease_sf.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
PfamiView protein in Pfam
PF00303. Thymidylat_synt. 1 hit.
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 2 hits.
PROSITEiView protein in PROSITE
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A259B4U7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKPPTTHPT RSQYEDFMRH VDTHGVFKAD RTGTGTKSVF GYQMRFDLNE
60 70 80 90 100
GFPLVTTKKV HLKSIVHELL WFLQGSSDNN WLKERGVTIW DEWARADGDL
110 120 130 140 150
GPVYGVQWRS WPTPDGGHID QISDVINTLK TNPDSRRIIV SAWNVADLSK
160 170 180 190 200
MALMPCHAFF QFYVAPATEP GGKGRLSCQL YQRSADIFLG VPFNIASYAL
210 220 230 240 250
LTHMVAQQCD LDVGDFIWTG GDCHIYSNHA EQVALQLSRA PLPYPTLQIK
260 270 280
RKPASIFDYQ FEDFEFSDYQ HHPAIKAPVA V
Length:281
Mass (Da):31,861
Last modified:November 22, 2017 - v1
Checksum:i46BF4AAD2AACAFC6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
NCHV01000035 Genomic DNA. Translation: OYZ84461.1.

Similar proteinsi

Entry informationi

Entry nameiA0A259B4U7_9BURK
AccessioniPrimary (citable) accession number: A0A259B4U7
Entry historyiIntegrated into UniProtKB/TrEMBL: November 22, 2017
Last sequence update: November 22, 2017
Last modified: February 28, 2018
This is version 4 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported