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Protein

Siroheme synthase

Gene

cobA

Organism
Salmonella enterica subsp. enterica serovar Saintpaul
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.UniRule annotationSAAS annotation

Catalytic activityi

2 S-adenosyl-L-methionine + uroporphyrinogen III = 2 S-adenosyl-L-homocysteine + precorrin-2.UniRule annotationSAAS annotation
Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotationSAAS annotation
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotationSAAS annotation

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG), Siroheme synthase (cobA)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sirohydrochlorin from precorrin-2, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG), Siroheme synthase (cobA)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes precorrin-2 from uroporphyrinogen III, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes siroheme from sirohydrochlorin.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG), Siroheme synthase (cobA)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes siroheme from sirohydrochlorin, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG), Siroheme synthase (cobA)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sirohydrochlorin from precorrin-2, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei225S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei248Proton acceptorUniRule annotation1
Active sitei270Proton donorUniRule annotation1
Binding sitei306S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei382S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1
Binding sitei411S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi22 – 23NADUniRule annotation2
Nucleotide bindingi43 – 44NADUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotation, MethyltransferaseUniRule annotationSAAS annotation, Multifunctional enzymeUniRule annotationSAAS annotation, OxidoreductaseUniRule annotationSAAS annotation, Transferase
Biological processCobalamin biosynthesisUniRule annotationSAAS annotation, Porphyrin biosynthesisUniRule annotationSAAS annotation
LigandNADUniRule annotationSAAS annotation, S-adenosyl-L-methionineUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00148; UER00222
UPA00262; UER00211
UPA00262; UER00222
UPA00262; UER00376

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme synthaseUniRule annotation
Including the following 3 domains:
Precorrin-2 dehydrogenaseUniRule annotation (EC:1.3.1.76UniRule annotation)
Sirohydrochlorin ferrochelataseUniRule annotation (EC:4.99.1.4UniRule annotation)
Uroporphyrinogen-III C-methyltransferaseUniRule annotation (EC:2.1.1.107UniRule annotation)
Short name:
Urogen III methylaseUniRule annotation
Alternative name(s):
SUMTUniRule annotation
Uroporphyrinogen III methylaseUniRule annotation
Short name:
UROMUniRule annotation
Gene namesi
Name:cobAImported
Synonyms:cysGUniRule annotation
ORF Names:CK947_01875Imported
OrganismiSalmonella enterica subsp. enterica serovar SaintpaulImported
Taxonomic identifieri90105 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000217166 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei128PhosphoserineUniRule annotation1

Keywords - PTMi

PhosphoproteinUniRule annotation

Structurei

3D structure databases

SMRiA0A249UTD2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini119 – 145Sirohm_synth_MInterPro annotationAdd BLAST27
Domaini150 – 207CysG_dimeriserInterPro annotationAdd BLAST58
Domaini218 – 426TP_methylaseInterPro annotationAdd BLAST209

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 204precorrin-2 dehydrogenase / sirohydrochlorin ferrochelataseUniRule annotationAdd BLAST204
Regioni216 – 457Uroporphyrinogen-III C-methyltransferaseUniRule annotationAdd BLAST242
Regioni301 – 303S-adenosyl-L-methionine bindingUniRule annotation3
Regioni331 – 332S-adenosyl-L-methionine bindingUniRule annotation2

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili185 – 205Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the precorrin methyltransferase family.UniRule annotation
In the C-terminal section; belongs to the precorrin methyltransferase family.UniRule annotationSAAS annotation
In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.UniRule annotationSAAS annotation

Keywords - Domaini

Coiled coilSequence analysis

Family and domain databases

CDDicd11642 SUMT, 1 hit
Gene3Di1.10.8.210, 1 hit
3.30.950.10, 1 hit
3.40.1010.10, 1 hit
HAMAPiMF_01646 Siroheme_synth, 1 hit
InterProiView protein in InterPro
IPR000878 4pyrrol_Mease
IPR035996 4pyrrol_Methylase_sf
IPR014777 4pyrrole_Mease_sub1
IPR014776 4pyrrole_Mease_sub2
IPR006366 CobA/CysG_C
IPR036291 NAD(P)-bd_dom_sf
IPR037115 Sirohaem_synt_dimer_dom_sf
IPR012409 Sirohaem_synth
IPR028281 Sirohaem_synthase_central
IPR019478 Sirohaem_synthase_dimer_dom
IPR006367 Sirohaem_synthase_N
IPR003043 Uropor_MeTrfase_CS
PfamiView protein in Pfam
PF10414 CysG_dimeriser, 1 hit
PF14824 Sirohm_synth_M, 1 hit
PF00590 TP_methylase, 1 hit
PIRSFiPIRSF036426 Sirohaem_synth, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF53790 SSF53790, 1 hit
TIGRFAMsiTIGR01469 cobA_cysG_Cterm, 1 hit
TIGR01470 cysG_Nterm, 1 hit
PROSITEiView protein in PROSITE
PS00839 SUMT_1, 1 hit
PS00840 SUMT_2, 1 hit

Sequencei

Sequence statusi: Complete.

A0A249UTD2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDHLPIFCQL RDRDCLIVGG GDVAERKARL LLEAGARLTV NALTFIPQFT
60 70 80 90 100
VWANEGMLTL VEGPFDETLL DSCWLAIAAT DDDTVNQRVS DAAESRRIFC
110 120 130 140 150
NVVDAPKAAS FIMPSIIDRS PLMVAVSSGG TSPVLARLLR EKLESLLPQH
160 170 180 190 200
LGQVARYAGQ LRARVKKQFA TMGERRRFWE KFFVNDRLAQ SLANADEKAV
210 220 230 240 250
NATTERLFSE PLDHRGEVVL VGAGPGDAGL LTLKGLQQIQ QADIVVYDRL
260 270 280 290 300
VSDDIMNLVR RDADRVFVGK RAGYHCVPQE EINQILLREA QKGKRVVRLK
310 320 330 340 350
GGDPFIFGRG GEELETLCHA GIPFSVVPGI TAASGCSAYS GIPLTHRDYA
360 370 380 390 400
QSVRLVTGHL KTGGELDWEN LAAEKQTLVF YMGLNQAATI QEKLIAFGMQ
410 420 430 440 450
ADMPVALVEN GTSVKQRVVH GVLTQLGELA QQVESPALII VGRVVALRDK

LNWFSNH
Length:457
Mass (Da):50,147
Last modified:December 20, 2017 - v1
Checksum:i359C8CAF4B78092D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP023166 Genomic DNA Translation: ASZ35410.1
RefSeqiWP_000349895.1, NZ_MXZU01000028.1

Similar proteinsi

Entry informationi

Entry nameiA0A249UTD2_SALET
AccessioniPrimary (citable) accession number: A0A249UTD2
Entry historyiIntegrated into UniProtKB/TrEMBL: December 20, 2017
Last sequence update: December 20, 2017
Last modified: June 20, 2018
This is version 7 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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