Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Histidine biosynthesis bifunctional protein HisIE

Gene

hisI

Organism
Shigella flexneri
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

1-(5-phospho-beta-D-ribosyl)-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide.UniRule annotationSAAS annotation
1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-AMP + diphosphate.UniRule annotationSAAS annotation

Pathwayi: L-histidine biosynthesis

This protein is involved in step 2 and 3 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotationSAAS annotation
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase (hisG), ATP phosphoribosyltransferase (hisG)
  2. Histidine biosynthesis bifunctional protein HisIE (hisI), Histidine biosynthesis bifunctional protein HisIE (hisI)
  3. Histidine biosynthesis bifunctional protein HisIE (hisI), Histidine biosynthesis bifunctional protein HisIE (hisI)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA), 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
  5. Imidazole glycerol phosphate synthase subunit HisH (hisH), Imidazole glycerol phosphate synthase subunit HisF (hisF), Imidazole glycerol phosphate synthase subunit HisH (hisH), Imidazole glycerol phosphate synthase subunit HisF (hisF)
  6. Histidine biosynthesis bifunctional protein HisB (hisB), Histidine biosynthesis bifunctional protein HisB (hisB)
  7. Histidinol-phosphate aminotransferase (hisC), Histidinol-phosphate aminotransferase (hisC)
  8. Histidine biosynthesis bifunctional protein HisB (hisB), Histidine biosynthesis bifunctional protein HisB (hisB)
  9. Histidinol dehydrogenase (hisD), Histidinol dehydrogenase (hisD)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotationImported
Biological processAmino-acid biosynthesis, Histidine biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00031; UER00007.
UPA00031; UER00008.

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine biosynthesis bifunctional protein HisIEUniRule annotation
Including the following 2 domains:
Phosphoribosyl-AMP cyclohydrolaseUniRule annotation (EC:3.5.4.19UniRule annotation)
Short name:
PRA-CHUniRule annotation
Phosphoribosyl-ATP pyrophosphataseUniRule annotation (EC:3.6.1.31UniRule annotation)
Short name:
PRA-PHUniRule annotation
Gene namesi
Name:hisIUniRule annotation
Synonyms:hisIEUniRule annotation
ORF Names:CBL25_08610Imported
OrganismiShigella flexneriImported
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000195900 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Structurei

3D structure databases

SMRiA0A200MSA2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 105PRA-CHInterPro annotationAdd BLAST73

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 114Phosphoribosyl-AMP cyclohydrolaseUniRule annotationAdd BLAST114
Regioni115 – 203Phosphoribosyl-ATP pyrophosphohydrolaseUniRule annotationAdd BLAST89

Sequence similaritiesi

In the C-terminal section; belongs to the PRA-PH family.UniRule annotationSAAS annotation
In the N-terminal section; belongs to the PRA-CH family.UniRule annotationSAAS annotation

Family and domain databases

CDDicd11534. NTP-PPase_HisIE_like. 1 hit.
HAMAPiMF_01020. HisE. 1 hit.
MF_01019. HisIE. 1 hit.
InterProiView protein in InterPro
IPR023019. His_synth_HisIE.
IPR008179. HisE.
IPR021130. PRib-ATP_PPHydrolase-like.
IPR002496. PRib_AMP_CycHydrolase_dom.
PfamiView protein in Pfam
PF01502. PRA-CH. 1 hit.
PF01503. PRA-PH. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD002610. PRA_CycHdrlase. 1 hit.
TIGRFAMsiTIGR03188. histidine_hisI. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A200MSA2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTEQQRREL DWEKTDGLMP VIVQHAVSGE VLMLGYMNPE ALDKTIESGK
60 70 80 90 100
VTFFSRTKQR LWIKGETSGN FLNVVSIAPD CDNDTLLVLA NPIGPTCHKG
110 120 130 140 150
TSSCFGNTAH QWLFLYQLEQ LLAERKYADP ETSYTAKLYA SGTKRIAQKV
160 170 180 190 200
GEEGVETALA ATVHDRFELT NEASDLMYHL LVLLQDQDLD LTTVIENLHK

RHQ
Length:203
Mass (Da):22,882
Last modified:October 25, 2017 - v1
Checksum:i51EC7BDA1D0BAEC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
NGWD01000029 Genomic DNA. Translation: OUZ76545.1.
RefSeqiWP_000954863.1. NZ_NMXZ01000030.1.

Similar proteinsi

Entry informationi

Entry nameiA0A200MSA2_SHIFL
AccessioniPrimary (citable) accession number: A0A200MSA2
Entry historyiIntegrated into UniProtKB/TrEMBL: October 25, 2017
Last sequence update: October 25, 2017
Last modified: December 20, 2017
This is version 3 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteomeImported, Multifunctional enzymeUniRule annotationSAAS annotation