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Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Shewanella sp. FDAARGOS_354
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotationSAAS annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Diaminopimelate epimerase (dapF)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12SubstrateUniRule annotation1
Binding sitei45SubstrateUniRule annotation1
Binding sitei65SubstrateUniRule annotation1
Active sitei74PROSITE-ProRule annotation1
Active sitei74Proton donorUniRule annotation1
Binding sitei158SubstrateUniRule annotation1
Sitei160Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Binding sitei191SubstrateUniRule annotation1
Sitei209Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Active sitei218Proton acceptorUniRule annotation1
Sitei269Important for dimerizationUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomeraseUniRule annotationSAAS annotation
Biological processAmino-acid biosynthesis, Lysine biosynthesisUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00034; UER00025.

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimeraseUniRule annotationSAAS annotation (EC:5.1.1.7UniRule annotationSAAS annotation)
Short name:
DAP epimeraseUniRule annotation
Alternative name(s):
PLP-independent amino acid racemaseUniRule annotation
Gene namesi
Name:dapFUniRule annotation
ORF Names:CEQ32_18455Imported
OrganismiShewanella sp. FDAARGOS_354Imported
Taxonomic identifieri1930557 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000197056 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni75 – 76Substrate bindingUniRule annotation2
Regioni209 – 210Substrate bindingUniRule annotation2
Regioni219 – 220Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the diaminopimelate epimerase family.UniRule annotationSAAS annotation

Phylogenomic databases

KOiK01778.

Family and domain databases

HAMAPiMF_00197. DAP_epimerase. 1 hit.
InterProiView protein in InterPro
IPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
PANTHERiPTHR31689. PTHR31689. 1 hit.
PfamiView protein in Pfam
PF01678. DAP_epimerase. 2 hits.
TIGRFAMsiTIGR00652. DapF. 1 hit.
PROSITEiView protein in PROSITE
PS01326. DAP_EPIMERASE. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A1Z4AFS0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIQFTKMHGL GNDFMVVDGV TQNVFFSPEQ IRRLADRNFG IGFDQLLLVE
60 70 80 90 100
PPYDPDLDFH YRIFNADGGE VENCGNGARC FARFVRNKGL TNKNKIRVST
110 120 130 140 150
SAGKMTLRLE RDGTVTVNMG VPVLEPSQIP FKAKKAEKTY LLQTPQQTFL
160 170 180 190 200
CGAASMGNPH CVLDVEDVTS ANVAEIGALL TKHERFPRGV NVGFMQVVNS
210 220 230 240 250
GHIKLRVYER GAAETLACGT GACAAVVVGQ IQGKLDQQVQ VDLPGGSLTI
260 270
NWEGEGKPLW MTGPAQHVYD GQIQL
Length:275
Mass (Da):30,082
Last modified:November 22, 2017 - v1
Checksum:iB8C056ACEC10E8EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP022089 Genomic DNA. Translation: ASF16785.1.
RefSeqiWP_037430212.1. NZ_CP022089.1.

Genome annotation databases

KEGGishf:CEQ32_18455.

Similar proteinsi

Entry informationi

Entry nameiA0A1Z4AFS0_9GAMM
AccessioniPrimary (citable) accession number: A0A1Z4AFS0
Entry historyiIntegrated into UniProtKB/TrEMBL: November 22, 2017
Last sequence update: November 22, 2017
Last modified: February 28, 2018
This is version 4 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported