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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Blautia sp. An249
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF), Amidophosphoribosyltransferase (B5F53_09615)
  2. Phosphoribosylamine--glycine ligase (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei15For GATase activityUniRule annotation1
Active sitei15NucleophileUniRule annotation1
Metal bindingi259Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi306MagnesiumUniRule annotation1
Metal bindingi368MagnesiumUniRule annotation1
Metal bindingi369MagnesiumUniRule annotation1
Metal bindingi405Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi454Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi457Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferaseUniRule annotationImported, Transferase
Biological processPurine biosynthesisUniRule annotation
Ligand4Fe-4SUniRule annotation, Iron, Iron-sulfur, MagnesiumUniRule annotation, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Short name:
GPATaseUniRule annotation
Gene namesi
Name:purFUniRule annotation
ORF Names:B5F53_11115Imported
OrganismiBlautia sp. An249Imported
Taxonomic identifieri1965603 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesLachnospiraceaeBlautia
Proteomesi
  • UP000195974 Componenti: Unassembled WGS sequence

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 241Glutamine amidotransferase type-2InterPro annotationAdd BLAST227

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferaseUniRule annotation

Family and domain databases

CDDicd00715. GPATase_N. 1 hit.
cd06223. PRTases_typeI. 1 hit.
Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF. 1 hit.
InterProiView protein in InterPro
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
IPR035584. PurF_N.
PfamiView protein in Pfam
PF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiView protein in PROSITE
PS51278. GATASE_TYPE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A1Y4FB89-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDLTNAQTG IKEACGVFGI YQFHKGNVFS LIHSGLYALQ HRGQEACGIA
60 70 80 90 100
VSDTEGERGN IRSRKGPGLV SEVFKNSKEE DFCGNLGIGH VRYSTTGASV
110 120 130 140 150
AENAQPLVLK YAKGTIALAH NGNLVNVSPL REELERAGTV FHTTTDSEVI
160 170 180 190 200
ACLIARERVR SHSVEEAVLK AVSRLNGAYG LVIMSPRKLI GVRDPWGLKP
210 220 230 240 250
LCLGKLEDAY LLASESCALT SIGAEFVRDI EPGELITITP DGITSKRFTH
260 270 280 290 300
PMAEKEARCI FEYIYFARLD STIDGVSVYE SRIQSGKALA RSHPVDADLV
310 320 330 340 350
IGVPDSGLTA AKGYSLQSGI PFALAFYKNS YVGRTFIKPT QEERQTAVSL
360 370 380 390 400
KLNVLASVVA GKRLVLVDDS IVRGTTIAAL IQMLKKAGAK EIHVRISSPP
410 420 430 440 450
FLHPCYFGTD VPSNRQLIAS SKTPEEICRS IGADSLGYLD LKDLPSIAGG
460
LPICKACFDA DYPMEIS
Length:467
Mass (Da):50,451
Last modified:August 30, 2017 - v1
Checksum:iC4C1ADF2756F404B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
NFJL01000011 Genomic DNA. Translation: OUO78100.1.

Similar proteinsi

Entry informationi

Entry nameiA0A1Y4FB89_9FIRM
AccessioniPrimary (citable) accession number: A0A1Y4FB89
Entry historyiIntegrated into UniProtKB/TrEMBL: August 30, 2017
Last sequence update: August 30, 2017
Last modified: October 25, 2017
This is version 3 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteomeImported