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Protein

Thymidylate synthase

Gene

thyA

Organism
Klebsiella pneumoniae subsp. pneumoniae
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotationSAAS annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21dUMPUniRule annotation1
Binding sitei515,10-methylenetetrahydrofolateUniRule annotation1
Active sitei146PROSITE-ProRule annotation1
Active sitei146NucleophileUniRule annotation1
Binding sitei1695,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei177dUMPUniRule annotation1
Binding sitei2635,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi126 – 127dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi166 – 169dUMPUniRule annotation4
Nucleotide bindingi207 – 209dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferaseUniRule annotationSAAS annotation, Transferase
Biological processNucleotide biosynthesisUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotationSAAS annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
ORF Names:AN663_06915Imported, AN676_0312305Imported, CI704_16050Imported, CXZ11_24760Imported
OrganismiKlebsiella pneumoniae subsp. pneumoniaeImported
Taxonomic identifieri72407 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella
Proteomesi
  • UP000050263 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 264Thymidylat_syntInterPro annotationAdd BLAST263

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

KOiK00560.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiView protein in InterPro
IPR023451. Thymidate_synth/dCMP_Mease.
IPR036926. Thymidate_synth/dCMP_Mease_sf.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
PfamiView protein in Pfam
PF00303. Thymidylat_synt. 1 hit.
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 2 hits.
PROSITEiView protein in PROSITE
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A1Y0PVG0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQYLDLMQK VLTEGTPKND RTGTGTISIF GHQMRFNLQE GFPLVTTKRC
60 70 80 90 100
HLRSIIHELL WFLQGDTNIA YLHENNVTIW DEWADENGDL GPVYGKQWRS
110 120 130 140 150
WPAPDGRHID QISTVMNQLK NDPDSRRIIV SAWNVGELDK MALAPCHAFF
160 170 180 190 200
QFYVADGKLS CQLYQRSCDV FLGLPFNIAS YALLVHMVAQ QCDLQVGDFV
210 220 230 240 250
WTGGDTHLYS NHLEQTNLQL SREPRPLPKL VIKRKPASIF DYRFEDFEIE
260
GYDPHPGIKA PVAI
Length:264
Mass (Da):30,293
Last modified:August 30, 2017 - v1
Checksum:iE5C48AD218062DAA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP023553 Genomic DNA. Translation: ATN99442.1.
CP023487 Genomic DNA. Translation: ATO04162.1.
CP025951 Genomic DNA. Translation: AUS23371.1.
NQDG01000039 Genomic DNA. Translation: PCK16141.1.
RefSeqiWP_002915933.1. NZ_PEEP01000039.1.

Genome annotation databases

KEGGikpg:KPNIH32_22485.
kpv:KPNIH29_21605.
kpw:KPNIH30_21625.
kpy:KPNIH31_20890.

Similar proteinsi

Entry informationi

Entry nameiA0A1Y0PVG0_KLEPN
AccessioniPrimary (citable) accession number: A0A1Y0PVG0
Entry historyiIntegrated into UniProtKB/TrEMBL: August 30, 2017
Last sequence update: August 30, 2017
Last modified: March 28, 2018
This is version 8 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported