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Protein

NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic

Gene

ndhF

Organism
Cucumis sativus (Cucumber)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient.UniRule annotation

Catalytic activityi

NAD(P)H + plastoquinone = NAD(P)+ + plastoquinol.UniRule annotationSAAS annotation

Keywordsi

Molecular functionOxidoreductaseUniRule annotationSAAS annotation
Biological processTransportUniRule annotation
LigandNADUniRule annotationSAAS annotation, NADPUniRule annotationSAAS annotation, PlastoquinoneUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
NAD(P)H-quinone oxidoreductase subunit 5, chloroplasticUniRule annotation (EC:1.6.5.-UniRule annotation)
Alternative name(s):
NADH-plastoquinone oxidoreductase subunit 5UniRule annotation
Gene namesi
Name:ndhFUniRule annotationImported
Encoded oniPlastid; ChloroplastImported
OrganismiCucumis sativus (Cucumber)Imported
Taxonomic identifieri3659 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeBenincaseaeCucumis

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei6 – 28HelicalUniRule annotationAdd BLAST23
Transmembranei40 – 60HelicalUniRule annotationAdd BLAST21
Transmembranei85 – 109HelicalUniRule annotationAdd BLAST25
Transmembranei121 – 139HelicalUniRule annotationAdd BLAST19
Transmembranei145 – 166HelicalUniRule annotationAdd BLAST22
Transmembranei187 – 203HelicalUniRule annotationAdd BLAST17
Transmembranei223 – 239HelicalUniRule annotationAdd BLAST17
Transmembranei260 – 281HelicalUniRule annotationAdd BLAST22
Transmembranei287 – 311HelicalUniRule annotationAdd BLAST25
Transmembranei327 – 348HelicalUniRule annotationAdd BLAST22
Transmembranei424 – 447HelicalUniRule annotationAdd BLAST24
Transmembranei549 – 570HelicalUniRule annotationAdd BLAST22
Transmembranei611 – 629HelicalUniRule annotationAdd BLAST19
Transmembranei728 – 747HelicalUniRule annotationAdd BLAST20

Keywords - Cellular componenti

ChloroplastUniRule annotationImported, Membrane, Plastid, ThylakoidUniRule annotation

PTM / Processingi

Keywords - PTMi

QuinoneUniRule annotation

Interactioni

Subunit structurei

NDH is composed of at least 16 different subunits, 5 of which are encoded in the nucleus.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 129Proton_antipo_NInterPro annotationAdd BLAST58
Domaini141 – 442Proton_antipo_MInterPro annotationAdd BLAST302
Domaini448 – 695Proton_antipo_CInterPro annotationAdd BLAST248

Sequence similaritiesi

Belongs to the complex I subunit 5 family.UniRule annotationSAAS annotation

Keywords - Domaini

Transmembrane, Transmembrane helixUniRule annotationSAAS annotation

Family and domain databases

InterProiView protein in InterPro
IPR002128. NADH_UbQ_OxRdtase_chlpt_su5_C.
IPR003945. NADHpl_OxRdtase_5.
IPR018393. NADHpl_OxRdtase_5_subgr.
IPR001750. ND/Mrp_mem.
IPR001516. Proton_antipo_N.
PfamiView protein in Pfam
PF01010. Proton_antipo_C. 1 hit.
PF00361. Proton_antipo_M. 1 hit.
PF00662. Proton_antipo_N. 1 hit.
PRINTSiPR01435. NPOXDRDTASE5.
TIGRFAMsiTIGR01974. NDH_I_L. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A1X9Q1P6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEYTYQYSWI IPFISLPIPI LIGVGLLLFP TATKNLRRLW SFPSILLLSI
60 70 80 90 100
VMIFAVYLSI DQINTSYIYQ YVWSWTINND FSLELGYFID PLTCIMAILI
110 120 130 140 150
TTVGIMVLIY SDNYMSHDQG YLRFFAYMSL FNTSMLGLVT SSNLIQIYIF
160 170 180 190 200
WELVGMCSYL LIGFWFTRPT AANACQKAFV TNRVGDFGLL LGILGLYWTT
210 220 230 240 250
GSFEFRDLFQ IFNNLIDNNE VNFLFLTLCA VLLFAGAVAK SAQFPLHVWL
260 270 280 290 300
PDAMEGPTPI SALIHAATMV AAGIFLVARL LPLFIAIPYI MNLIALIGII
310 320 330 340 350
TVLFGATLAL AQKDIKRSLA YSTMSQLGYM MLALGMGSYQ AALFHLITHA
360 370 380 390 400
YSKALLFLGS GSIIHSMESV VGYSPDKSQN MVLMGGLTKH VPITKNAFLL
410 420 430 440 450
GTLSLCGVPP LACFWSKDEI LNDSWLYSPI FAIIACSTAG LTAFYMFRIY
460 470 480 490 500
LLTFDGHLNL YCKNYSGKTN NSLYSISLWG KEGSKIIKKN FPFFSLLTMN
510 520 530 540 550
NKERSSFWMK KTTYPFNSNV TKKMRPFVSS PHFSTKNTFY YPYESENTML
560 570 580 590 600
FPMFVLSLFT LFVGVIGSPF TQFNQEAIDL NILDKWLTPS INLLHESSKD
610 620 630 640 650
SENWYEFVKN ATFSVTIAFF GIFIASFFYK PTYSSLQNLN FRNSFVKSFT
660 670 680 690 700
KNFFFEKIIN IIYNWSYNRG YIDTFYRISL IGGIRILAEL TYFFDRRIID
710 720 730 740
GITNAVGILN FFVAESIKYV GGGRISSYIL LYLVYLVIFI LVIYFVFF
Length:748
Mass (Da):85,207
Last modified:August 30, 2017 - v1
Checksum:i2D0F298D51C06C68
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KX231327 Genomic DNA. Translation: ARQ16125.1.
KX231328 Genomic DNA. Translation: ARQ16208.1.
KX231329 Genomic DNA. Translation: ARQ16291.1.
KX231330 Genomic DNA. Translation: ARQ16374.1.

Similar proteinsi

Entry informationi

Entry nameiA0A1X9Q1P6_CUCSA
AccessioniPrimary (citable) accession number: A0A1X9Q1P6
Entry historyiIntegrated into UniProtKB/TrEMBL: August 30, 2017
Last sequence update: August 30, 2017
Last modified: September 27, 2017
This is version 2 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)