Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Mycobacterium iranicum
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei81SubstrateUniRule annotation1
Metal bindingi229ManganeseUniRule annotation1
Metal bindingi249Manganese; via tele nitrogenUniRule annotation1
Binding sitei271SubstrateUniRule annotation1
Active sitei273UniRule annotation1
Metal bindingi296ManganeseUniRule annotation1
Binding sitei389GTPUniRule annotation1
Binding sitei420GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi272 – 277GTPUniRule annotation6
Nucleotide bindingi515 – 518GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylaseUniRule annotationSAAS annotation, KinaseImported, Lyase, Transferase
Biological processGluconeogenesisUniRule annotation
LigandGTP-bindingUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PyruvateImported

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Alternative name(s):
GTP-dependent phosphoenolpyruvate carboxykinaseUniRule annotation
Short name:
GTP-PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
ORF Names:AWC12_28225Imported
OrganismiMycobacterium iranicumImported
Taxonomic identifieri912594 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000193622 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 240PEPCK_NInterPro annotationAdd BLAST219
Domaini245 – 605PEPCK_CInterPro annotationAdd BLAST361

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni220 – 222Substrate bindingUniRule annotation3
Regioni387 – 389Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Family and domain databases

CDDicd00819. PEPCK_GTP. 1 hit.
Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiView protein in InterPro
IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR035077. PEP_carboxykinase_GTP_C.
IPR035078. PEP_carboxykinase_GTP_N.
IPR008210. PEP_carboxykinase_N.
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiView protein in Pfam
PF00821. PEPCK_C. 1 hit.
PF17297. PEPCK_N. 1 hit.
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiView protein in PROSITE
PS00505. PEPCK_GTP. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A1X1W7C1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAATIPGLD SAPTNHQGLL AWVREVAELT QPDRVAWADG SEEEYERVCA
60 70 80 90 100
QLVEAGTFQK LNPDKQPNSY LALSDPSDVA RVESRTYICS QREIDAGPTN
110 120 130 140 150
NWMDPAEMRG IMTDLYRGSM RGRTMWVVPF CMGPLDAEDP KLGVEITDSE
160 170 180 190 200
YVVVSMRTMT RMGAAALEKL GEDGFFVKAL HSLGAPLEPG QKDVPWPCND
210 220 230 240 250
TKYITHFPET REIWSYGSGY GGNALLGKKC YSLRIASAMA HDEGWLAEHM
260 270 280 290 300
LILKLISPEN KAYYIAAAFP SACGKTNLAM LQPTIPGWRA ETVGDDIAWM
310 320 330 340 350
RFGKDGRLYA TNPEFGFFGV APGTNWDSNP NAMKTIAGGN TVFTNVAKTD
360 370 380 390 400
DGDVWWEGLE GEPDHLIDWK GNDWILRETE TKAAHPNSRY CTPIAQCPTL
410 420 430 440 450
APEWDDPQGV PISAILFGGR RKTTVPLITE ARDWQHGVFI GATLGSEQTA
460 470 480 490 500
AAEGKVGTVR RDPMAMLPFL GYNVGDYFQH WINIGKNADE SQLPKVFFVN
510 520 530 540 550
WFRRGDDGRF LWPGFGENSR VLKWAVERIE HTAEGKSTPI GIVPTASDLD
560 570 580 590 600
LEGLDVDADD VDLALAVNAE EWRKELPLIE EWFEFVGEKL PTGIKDEFDA

LKTRLAEES
Length:609
Mass (Da):67,566
Last modified:July 5, 2017 - v1
Checksum:i9824D388A947467E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LQPC01000065 Genomic DNA. Translation: ORV82486.1.
RefSeqiWP_024449364.1. NZ_LQPC01000065.1.

Similar proteinsi

Entry informationi

Entry nameiA0A1X1W7C1_9MYCO
AccessioniPrimary (citable) accession number: A0A1X1W7C1
Entry historyiIntegrated into UniProtKB/TrEMBL: July 5, 2017
Last sequence update: July 5, 2017
Last modified: September 27, 2017
This is version 3 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteomeImported