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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA1

Organism
Syntrophorhabdus sp. PtaU1.Bin058
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. 6-phosphofructokinase (pfp), ATP-dependent 6-phosphofructokinase (pfkA1)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13ATP; via amide nitrogenUniRule annotation1
Metal bindingi118Magnesium; catalyticUniRule annotation1
Sitei119Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei142Proton acceptorUniRule annotation1
Binding sitei177Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei237SubstrateUniRule annotation1
Binding sitei281Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi76 – 77ATPUniRule annotation2
Nucleotide bindingi117 – 120ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinaseUniRule annotationSAAS annotationImported, Transferase
Biological processGlycolysisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkA1Imported
Synonyms:pfkAUniRule annotation
ORF Names:A4E64_03237Imported
OrganismiSyntrophorhabdus sp. PtaU1.Bin058Imported
Taxonomic identifieri1811704 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaSyntrophobacteralesSyntrophorhabdaceaeSyntrophorhabdus

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 312PFKInterPro annotationAdd BLAST308

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni140 – 142Substrate bindingUniRule annotation3
Regioni184 – 186Substrate bindingUniRule annotation3
Regioni287 – 290Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III. 1 hit.
InterProiView protein in InterPro
IPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
IPR035966. PKF_sf.
PfamiView protein in Pfam
PF00365. PFK. 1 hit.
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiView protein in PROSITE
PS00433. PHOSPHOFRUCTOKINASE. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A1V5CL31-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGKKIAINT GGGDAPGLNA VIEAVVMSAS KRNWEVYGIK NGYTGLLNIN
60 70 80 90 100
EIVRLTPEMV RGISSMGGTI IGTTNKGNPF EMPISNATGG IEIRDISDKV
110 120 130 140 150
VENFNRLGFD CLVAVGGDGS LKIAYDFFKK GVPVVGVPKT IDNDLGGTVI
160 170 180 190 200
TFGFDTAVSI ATEAIDRLHS TARSHDRVMV VEVMGRHAGW IALNSGVSGG
210 220 230 240 250
ADVILLPEIP FDVDRICKHI MDNELHGRHY AIVVAGEGAA PIGGAELSKG
260 270 280 290 300
PGEVGRQDVV LGGVGEFVAR AIAEKTGKDT RSIVLGHLQR GGSPTTFDRL
310 320 330 340 350
VSLRFGAAAV RMVETGQFGV MVALEPPVMK GVPLADVIGK IRRVSLDSDS
360
VQTARELGIC LGD
Length:363
Mass (Da):38,142
Last modified:June 7, 2017 - v1
Checksum:i8C9816387E366080
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
MVRS01000263 Genomic DNA. Translation: OPY71528.1.

Similar proteinsi

Entry informationi

Entry nameiA0A1V5CL31_9DELT
AccessioniPrimary (citable) accession number: A0A1V5CL31
Entry historyiIntegrated into UniProtKB/TrEMBL: June 7, 2017
Last sequence update: June 7, 2017
Last modified: October 25, 2017
This is version 5 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported