rpoA

Functionⁱ

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.UniRule annotation

Catalytic activityⁱ

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functionⁱ

DNA binding Source: UniProtKB-UniRule
DNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-UniRule
protein dimerization activity Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

transcription, DNA-templated Source: UniProtKB-UniRule

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	NucleotidyltransferaseUniRule annotation, Transferase
Biological process	Transcription

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: DNA-directed RNA polymerase subunit alphaUniRule annotation (EC:2.7.7.6UniRule annotation) Short name: RNAP subunit alphaUniRule annotation Alternative name(s): RNA polymerase subunit alphaUniRule annotation Transcriptase subunit alphaUniRule annotation
Gene namesⁱ	Name:rpoAUniRule annotation ORF Names:B2I22_12845Imported
Organismⁱ	Bacillus intestinalisImported
Taxonomic identifierⁱ	1963032 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Terrabacteria group › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus
Proteomesⁱ	UP000189784 Componentⁱ: Unassembled WGS sequence

Subcellular locationⁱ

Keywords - Cellular componentⁱ

DNA-directed RNA polymeraseUniRule annotationImported

Interactionⁱ

Subunit structureⁱ

Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.UniRule annotation

GO - Molecular functionⁱ

protein dimerization activity Source: InterPro

View the complete GO annotation on QuickGO ...

Structureⁱ

3D structure databases

SMRⁱ	A0A1V4FUC0.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	20 – 227	RPOLDInterPro annotationAdd BLAST		208

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	1 – 230	Alpha N-terminal domain (alpha-NTD)UniRule annotationAdd BLAST		230
Regionⁱ	247 – 314	Alpha C-terminal domain (alpha-CTD)UniRule annotationAdd BLAST		68

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	231 – 251	Sequence analysisAdd BLAST		21

Domainⁱ

The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements.UniRule annotation

Sequence similaritiesⁱ

Belongs to the RNA polymerase alpha chain family.UniRule annotation

Keywords - Domainⁱ

Coiled coilSequence analysis

Family and domain databases

Gene3Dⁱ	2.170.120.12. 1 hit. 3.30.1360.10. 2 hits.
HAMAPⁱ	MF_00059. RNApol_bact_RpoA. 1 hit.
InterProⁱ	View protein in InterPro IPR011262. DNA-dir_RNA_pol_insert. IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3. IPR011773. DNA-dir_RpoA. IPR036603. RBP11-like. IPR011260. RNAP_asu_C. IPR036643. RNApol_insert_sf.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01000. RNA_pol_A_bac. 1 hit. PF03118. RNA_pol_A_CTD. 1 hit. PF01193. RNA_pol_L. 1 hit.
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD001179. RNAP_asu_C. 1 hit.
SMARTⁱ	View protein in SMART SM00662. RPOLD. 1 hit.
SUPFAMⁱ	SSF55257. SSF55257. 2 hits. SSF56553. SSF56553. 1 hit.
TIGRFAMsⁱ	TIGR02027. rpoA. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

A0A1V4FUC0-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MIEIEKPKIE TVEISDDAKF GKFVVEPLER GYGTTLGNSL RRILLSSLPG 
        60         70         80         90        100
AAVTSIQIDG VLHEFSTIEG VVEDVTTIIL HIKKLALKIY SDEEKTLEID 
       110        120        130        140        150
VQGEGTVTAA DITHDSDVEI LNPDLHIATL GENASFRVRL TAQRGRGYTP 
       160        170        180        190        200
ADANKRDDQP IGVIPIDSIY TPVSRVSYQV ENTRVGQVAN YDKLTLDVWT 
       210        220        230        240        250
DGSTGPKEAI ALGSKILTEH LNIFVGLTDE AQHAEIMVEK EEDQKEKVLE 
       260        270        280        290        300
MTIEELDLSV RSYNCLKRAG INTVQELANK TEEDMMKVRN LGRKSLEEVK 
       310 
AKLEELGLGL RKDD

Length:314

Mass (Da):34,799

Last modified:June 7, 2017 - v1

Checksum:ⁱ9ADCAD891C0BCD67

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	MWZB01000077 Genomic DNA. Translation: OPG91220.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_003225835.1. NZ_MWZB01000077.1.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
A0A1V4FUC0	P20429 A0A063XB83 A0A199W9X2 D4G4D4 G4NQH6 A0A1Y0WH60 A0A125UDB7 L8AC64 A0A0K6JSL1 A0A0T8Q3S4 +7	Bacillus subtilis (strain 168) Bacillus subtilis Bacillus subtilis subsp. subtilis Bacillus subtilis subsp. natto (strain BEST195) Bacillus subtilis subsp. spizizenii (strain TU-B-10) Bacillus licheniformis Bacillus sp. LM 4-2 Bacillus subtilis BEST7613 Bacillus cereus Streptococcus pneumoniae And more	314	UniRef100_P20429	Cluster: DNA-directed RNA polymerase subunit alpha	18

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
A0A1V4FUC0	P20429 A0A063XB83 A0A199W9X2 D4G4D4 G4NQH6 A0A1Y0WH60 A0A125UDB7 L8AC64 A0A0K6JSL1 A0A0T8Q3S4 +460	Bacillus subtilis (strain 168) Bacillus subtilis Bacillus subtilis subsp. subtilis Bacillus subtilis subsp. natto (strain BEST195) Bacillus subtilis subsp. spizizenii (strain TU-B-10) Bacillus licheniformis Bacillus sp. LM 4-2 Bacillus subtilis BEST7613 Bacillus cereus Streptococcus pneumoniae And more	314	UniRef90_P20429	Cluster: DNA-directed RNA polymerase subunit alpha	471

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
A0A1V4FUC0	P20429 A0A063XB83 A0A199W9X2 D4G4D4 G4NQH6 A0A1Y0WH60 A0A125UDB7 L8AC64 A0A0K6JSL1 A0A0T8Q3S4 +4263	Bacillus subtilis (strain 168) Bacillus subtilis Bacillus subtilis subsp. subtilis Bacillus subtilis subsp. natto (strain BEST195) Bacillus subtilis subsp. spizizenii (strain TU-B-10) Bacillus licheniformis Bacillus sp. LM 4-2 Bacillus subtilis BEST7613 Bacillus cereus Streptococcus pneumoniae And more	314	UniRef50_P20429	Cluster: DNA-directed RNA polymerase subunit alpha	4,274

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	MWZB01000077 Genomic DNA. Translation: OPG91220.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_003225835.1. NZ_MWZB01000077.1.

3D structure databases

SMRⁱ	A0A1V4FUC0.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Family and domain databases

Gene3Dⁱ	2.170.120.12. 1 hit. 3.30.1360.10. 2 hits.
HAMAPⁱ	MF_00059. RNApol_bact_RpoA. 1 hit.
InterProⁱ	View protein in InterPro IPR011262. DNA-dir_RNA_pol_insert. IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3. IPR011773. DNA-dir_RpoA. IPR036603. RBP11-like. IPR011260. RNAP_asu_C. IPR036643. RNApol_insert_sf.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01000. RNA_pol_A_bac. 1 hit. PF03118. RNA_pol_A_CTD. 1 hit. PF01193. RNA_pol_L. 1 hit.
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD001179. RNAP_asu_C. 1 hit.
SMARTⁱ	View protein in SMART SM00662. RPOLD. 1 hit.
SUPFAMⁱ	SSF55257. SSF55257. 2 hits. SSF56553. SSF56553. 1 hit.
TIGRFAMsⁱ	TIGR02027. rpoA. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	A0A1V4FUC0_9BACI
Accessionⁱ	A0A1V4FUC0Primary (citable) accession number: A0A1V4FUC0
Entry historyⁱ	Integrated into UniProtKB/TrEMBL:	June 7, 2017
	Last sequence update:	June 7, 2017
	Last modified:	February 28, 2018
	This is version 7 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Unreviewed (UniProtKB/TrEMBL)

Miscellaneousⁱ

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termⁱ

Complete proteomeImported

UniProtKB - A0A1V4FUC0 (A0A1V4FUC0_9BACI)

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DNA-directed RNA polymerase subunit alpha

Select a section on the left to see content.

Functionⁱ

Catalytic activityⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Keywordsⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Interactionⁱ

Subunit structureⁱ

GO - Molecular functionⁱ

Structureⁱ

3D structure databases

Family & Domainsⁱ

Domains and Repeats

Region

Coiled coil

Domainⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Family and domain databases

Sequenceⁱ

Sequence databases

Similar proteinsⁱ

Cross-referencesⁱ

Sequence databases

3D structure databases

Protocols and materials databases

Family and domain databases

Entry informationⁱ

Miscellaneousⁱ

Caution

Keywords - Technical termⁱ

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - A0A1V4FUC0 (A0A1V4FUC0_9BACI)

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DNA-directed RNA polymerase subunit alpha

rpoA

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Coiled coil

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Family and domain databases

Sequence databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protocols and materials databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Caution

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ