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Protein

Thiazole synthase

Gene

thiG

Organism
Bradyrhizobium sp. AS23.2
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotationSAAS annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotationSAAS annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei96Schiff-base intermediate with DXPUniRule annotation1
Binding sitei157DXP; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotationSAAS annotation
Biological processThiamine biosynthesisUniRule annotationSAAS annotation
LigandSchiff baseUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotationSAAS annotation (EC:2.8.1.10UniRule annotationSAAS annotation)
Gene namesi
Name:thiGUniRule annotation
ORF Names:AC630_23040Imported
OrganismiBradyrhizobium sp. AS23.2Imported
Taxonomic identifieri1680155 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000186850 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 249ThiGInterPro annotationAdd BLAST246

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni184 – 185DXP bindingUniRule annotation2
Regioni206 – 207DXP bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotationSAAS annotation

Family and domain databases

CDDicd04728 ThiG, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00443 ThiG, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR033983 Thiazole_synthase_ThiG
IPR008867 ThiG
PANTHERiPTHR34266 PTHR34266, 1 hit
PfamiView protein in Pfam
PF05690 ThiG, 1 hit

Sequencei

Sequence statusi: Complete.

A0A1Q5RKQ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTFYGKTFA SRLLIGSALY PSPAIMQAAI RASGSNIVTV SLRREAAGGK
60 70 80 90 100
TGDAFWKLIR ELDVTVLPNT AGCRSVREAV TTAKLARELF GTSWIKLEVI
110 120 130 140 150
ADNDTLQPDV VGLVEAAAIL IKDGFEVFPY CTEDLSVANR LVDAGCKVVM
160 170 180 190 200
PWAAPIGSAK GIINRDALRL LRERLPDITL VVDAGLGAPS HAAHALELGY
210 220 230 240 250
DAVLLNTAIA KAADPVAMAN AFRLGVDAGR TAYEAGLMNA RDFASPSTPV
260
VGTPFWHAVS
Length:260
Mass (Da):27,493
Last modified:April 12, 2017 - v1
Checksum:i0B87BAD8F2826791
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LGHM01000113 Genomic DNA Translation: OKO76313.1
RefSeqiWP_074120847.1, NZ_LGHM01000113.1

Genome annotation databases

GeneIDi32150076

Similar proteinsi

Entry informationi

Entry nameiA0A1Q5RKQ9_9BRAD
AccessioniPrimary (citable) accession number: A0A1Q5RKQ9
Entry historyiIntegrated into UniProtKB/TrEMBL: April 12, 2017
Last sequence update: April 12, 2017
Last modified: April 25, 2018
This is version 11 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
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Main funding by: National Institutes of Health