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Protein

tRNA sulfurtransferase

Gene

thiI

Organism
Pseudomonas sp. 7SR1
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotationSAAS annotation

Catalytic activityi

ATP + [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uracil in tRNA + 2 reduced ferredoxin [iron-sulfur] cluster = AMP + diphosphate + 4-thiouracil in tRNA + [ThiI sulfur-carrier protein]-L-cysteine + 2 oxidized ferredoxin [iron-sulfur] cluster.UniRule annotationSAAS annotation
[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotationSAAS annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei267ATPUniRule annotation1
Binding sitei289ATP; via amide nitrogenUniRule annotation1
Binding sitei298ATPUniRule annotation1
Active sitei457Cysteine persulfide intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi185 – 186ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding, TransferaseUniRule annotationSAAS annotationImported, tRNA-bindingUniRule annotationSAAS annotation
Biological processThiamine biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA sulfurtransferaseUniRule annotationSAAS annotation (EC:2.8.1.4UniRule annotationSAAS annotation)
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
Thiamine biosynthesis protein ThiIUniRule annotation
tRNA 4-thiouridine synthaseUniRule annotation
Gene namesi
Name:thiIUniRule annotation
ORF Names:SAMN05428955_1075Imported
OrganismiPseudomonas sp. 7SR1Imported
Taxonomic identifieri1881017 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000186061 Componenti: Chromosome i

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

PTM / Processingi

Keywords - PTMi

Disulfide bondSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 167THUMPInterPro annotationAdd BLAST105
Domaini405 – 483RhodaneseInterPro annotationAdd BLAST79

Sequence similaritiesi

Belongs to the ThiI family.UniRule annotationSAAS annotation

Keywords - Domaini

Redox-active centerSAAS annotation

Family and domain databases

CDDicd01712. ThiI. 1 hit.
Gene3Di3.40.250.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI. 1 hit.
InterProiView protein in InterPro
IPR001763. Rhodanese-like_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR026340. Thiazole_biosynth_dom.
IPR020536. ThiI_AANH.
IPR004114. THUMP_dom.
IPR003720. tRNA_STrfase.
PfamiView protein in Pfam
PF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
SMARTiView protein in SMART
SM00981. THUMP. 1 hit.
SUPFAMiSSF52821. SSF52821. 1 hit.
TIGRFAMsiTIGR04271. ThiI_C_thiazole. 1 hit.
TIGR00342. TIGR00342. 1 hit.
PROSITEiView protein in PROSITE
PS50206. RHODANESE_3. 1 hit.
PS51165. THUMP. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A1N7FLS3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLIVKVFPE ITIKSRPVRT RFIRQLAKNI RAVLRDLDPV VVVNGVWDNL
60 70 80 90 100
ELETRVSEPR ILKEMTERLS CMPGIAHFLQ VDEYPLGDFD DIVAKCKLHY
110 120 130 140 150
GDALAGKIFS VRCKRAGKHP FSSMDVEKYV GSRLRQQCGA AGISLKEPQV
160 170 180 190 200
EVRIEIRDQR LFVIHSQHDS IGGYPLGALE QTLVLMSGGF DSTVAAYQIM
210 220 230 240 250
RRGLMSHFCF FNLGGRAHEL GVMEVAHFIW KKYGSSQRVL FVSVPFEEVL
260 270 280 290 300
GEILGKVDNS HMGVILKRMM LRAASSIADR LHIDALVTGE AISQVSSQTL
310 320 330 340 350
PNLSVIDCVT EKLVLRPLIA SHKQDIIDTA TAIGTAEFAR HMPEYCGVIS
360 370 380 390 400
VNPKTAAKRG RVEHEEKEFD MAVLERALEN ARLVPIDRVI DELGQDLQVE
410 420 430 440 450
EVGEALAGQI VIDIRHPDEA EDDPLSVAGV EVKTMPFYAV NARFKELDPT
460 470 480
RQYLLYCGKG VMSRLHAHHL LSEGHANVRV YRPS
Length:484
Mass (Da):54,164
Last modified:March 15, 2017 - v1
Checksum:i1EC19223FC7A5418
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LT707064 Genomic DNA. Translation: SIS01348.1.
RefSeqiWP_072397932.1. NZ_LT707064.1.

Similar proteinsi

Entry informationi

Entry nameiA0A1N7FLS3_9PSED
AccessioniPrimary (citable) accession number: A0A1N7FLS3
Entry historyiIntegrated into UniProtKB/TrEMBL: March 15, 2017
Last sequence update: March 15, 2017
Last modified: September 27, 2017
This is version 6 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteomeImported