Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bifunctional protein GlmU

Gene

glmU_1

Organism
Mycobacterium abscessus subsp. abscessus
Status
Unreviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.UniRule annotationSAAS annotation

Catalytic activityi

Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotationSAAS annotation
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: LPS lipid A biosynthesis

This protein is involved in the pathway LPS lipid A biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway LPS lipid A biosynthesis and in Bacterial outer membrane biogenesis.

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II).UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Bifunctional protein GlmU (glmU_1), Bifunctional protein GlmU (glmU_3), Bifunctional protein GlmU (glmU), Bifunctional protein GlmU (glmU), Bifunctional protein GlmU (glmU_3), Bifunctional protein GlmU (glmU_3), Bifunctional protein GlmU (glmU_2), Bifunctional protein GlmU (glmU_2), Bifunctional protein GlmU (glmU_2), Bifunctional protein GlmU (glmU), Bifunctional protein GlmU (glmU_2), Bifunctional protein GlmU (glmU_2), Bifunctional protein GlmU (glmU_2), Bifunctional protein GlmU (glmU_1), Bifunctional protein GlmU (glmU_2), Bifunctional protein GlmU (glmU_1), Bifunctional protein GlmU (glmU_1), Bifunctional protein GlmU (glmU_4), Bifunctional protein GlmU (glmU_1)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II), the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional protein GlmU (glmU_1), Bifunctional protein GlmU (glmU_3), Bifunctional protein GlmU (glmU), Bifunctional protein GlmU (glmU), Bifunctional protein GlmU (glmU_3), Bifunctional protein GlmU (glmU_3), Bifunctional protein GlmU (glmU_2), Bifunctional protein GlmU (glmU_2), Bifunctional protein GlmU (glmU_2), Bifunctional protein GlmU (glmU), Bifunctional protein GlmU (glmU_2), Bifunctional protein GlmU (glmU_2), Bifunctional protein GlmU (glmU_2), Bifunctional protein GlmU (glmU_1), Bifunctional protein GlmU (glmU_2), Bifunctional protein GlmU (glmU_1), Bifunctional protein GlmU (glmU_1), Bifunctional protein GlmU (glmU_4), Bifunctional protein GlmU (glmU_1)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate, the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei27UDP-GlcNAcUniRule annotation1
Binding sitei84UDP-GlcNAcUniRule annotation1
Metal bindingi115MagnesiumUniRule annotation1
Binding sitei152UDP-GlcNAc; via amide nitrogenUniRule annotation1
Binding sitei167UDP-GlcNAcUniRule annotation1
Binding sitei182UDP-GlcNAcUniRule annotation1
Metal bindingi240MagnesiumUniRule annotation1
Binding sitei240UDP-GlcNAcUniRule annotation1
Binding sitei345UDP-GlcNAcUniRule annotation1
Binding sitei363UDP-GlcNAcUniRule annotation1
Active sitei375Proton acceptorUniRule annotation1
Binding sitei378UDP-GlcNAcUniRule annotation1
Binding sitei389UDP-GlcNAcUniRule annotation1
Binding sitei392Acetyl-CoA; via amide nitrogenUniRule annotation1
Binding sitei417Acetyl-CoAUniRule annotation1
Binding sitei435Acetyl-CoA; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferaseUniRule annotationSAAS annotation, NucleotidyltransferaseUniRule annotationSAAS annotation, Transferase
Biological processCell shape, Cell wall biogenesis/degradationUniRule annotationSAAS annotation, Peptidoglycan synthesisUniRule annotationSAAS annotation
LigandMagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00113; UER00532.
UPA00113; UER00533.
UPA00973.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein GlmUUniRule annotation
Including the following 2 domains:
UDP-N-acetylglucosamine pyrophosphorylaseUniRule annotation (EC:2.7.7.23UniRule annotation)
Alternative name(s):
N-acetylglucosamine-1-phosphate uridyltransferaseUniRule annotation
Glucosamine-1-phosphate N-acetyltransferaseUniRule annotation (EC:2.3.1.157UniRule annotation)
Gene namesi
Name:glmU_1Imported
Synonyms:glmUUniRule annotationImported
ORF Names:SAMEA2070424_00430Imported, SAMEA2275679_00826Imported
OrganismiMycobacterium abscessus subsp. abscessusImported
Taxonomic identifieri1185650 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium abscessus

Subcellular locationi

A0A1N0BC91:
  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotrimer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 143NTP_transf_3InterPro annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 242PyrophosphorylaseUniRule annotationAdd BLAST242
Regioni13 – 16UDP-GlcNAc bindingUniRule annotation4
Regioni89 – 90UDP-GlcNAc bindingUniRule annotation2
Regioni113 – 115UDP-GlcNAc bindingUniRule annotation3
Regioni243 – 263LinkerUniRule annotationAdd BLAST21
Regioni264 – 482N-acetyltransferaseUniRule annotationAdd BLAST219
Regioni398 – 399Acetyl-CoA bindingUniRule annotation2

Sequence similaritiesi

In the C-terminal section; belongs to the transferase hexapeptide repeat family.UniRule annotationSAAS annotation
In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.UniRule annotationSAAS annotation

Keywords - Domaini

RepeatUniRule annotationSAAS annotation

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_01631. GlmU. 1 hit.
InterProiView protein in InterPro
IPR005882. Bifunctional_GlmU.
IPR001451. Hexapep.
IPR025877. MobA-like_NTP_Trfase.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
PfamiView protein in Pfam
PF00132. Hexapep. 3 hits.
PF12804. NTP_transf_3. 1 hit.
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01173. glmU. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A1N0BC91-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGNTAGTAV IVLAAGAGTR MCSDIPKVLH TLGGRSMLAH AVYAAASVNP
60 70 80 90 100
EHLVIVLGHE RERIATAVDI LAESLGRRIE IAVQEQQLGT GHAVACGLQA
110 120 130 140 150
LPAGFAGTVV VTSGDIPLLD GGTLAGLIGS HTSEPAAATL LTTTLTDPTG
160 170 180 190 200
YGRILRTQDR EVIAIVEQTD ATDSQRAIGE VNAGVYAFDI EPLHAALSRL
210 220 230 240 250
RSNNAQHELY LTDAVSIIRE SGKVVHANHV DDSALVAGVN DRVQLSELGA
260 270 280 290 300
ELNRRIIRHH QRNGVTIIDP SSTWIDVDVQ IGQDATIAPG TQLHSATVIG
310 320 330 340 350
GHCHIGPDTT LIDVTVGDSA TVIRTHGQGS TIGARSVIGP FTYLRPGTVT
360 370 380 390 400
GESAKLGAFV EVKNSTVGRG TKVPHLTYVG DADIGEHSNI GASSVFVNYD
410 420 430 440 450
GETKRRTVIG SHVKTGSDTM FVAPVTVGDG AYTGAGTVIR EDVPPGALAV
460 470 480
SAGRQRNIEG WVAQKRPGSD AAKAAEEASK GS
Length:482
Mass (Da):49,979
Last modified:March 15, 2017 - v1
Checksum:i327AC1633B79A107
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FSAB01000001 Genomic DNA. Translation: SHP05508.1.
FSFO01000001 Genomic DNA. Translation: SHX82340.1.
RefSeqiWP_017207044.1. NZ_FWCW01000011.1.

Similar proteinsi

Entry informationi

Entry nameiA0A1N0BC91_9MYCO
AccessioniPrimary (citable) accession number: A0A1N0BC91
Entry historyiIntegrated into UniProtKB/TrEMBL: March 15, 2017
Last sequence update: March 15, 2017
Last modified: September 27, 2017
This is version 6 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Multifunctional enzymeUniRule annotationSAAS annotation