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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Sphingomonas sp. 66-10
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotationSAAS annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotation, NucleotidyltransferaseUniRule annotationSAAS annotationImported, Transferase
LigandMagnesiumUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
Gene namesi
Name:glnDUniRule annotation
ORF Names:BGN95_24425Imported
OrganismiSphingomonas sp. 66-10Imported
Taxonomic identifieri1895848 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingomonas
Proteomesi
  • UP000184439 Componenti: Unassembled WGS sequence

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini726 – 808ACTInterPro annotationAdd BLAST83
Domaini837 – 912ACTInterPro annotationAdd BLAST76

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 372UridylyltransferaseUniRule annotationAdd BLAST372

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotationSAAS annotation

Keywords - Domaini

RepeatSAAS annotation

Family and domain databases

CDDicd00077. HDc. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf. 1 hit.
InterProiView protein in InterPro
IPR002912. ACT_dom.
IPR005105. GlnD_Uridyltrans_N.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
IPR010043. UTase/UR.
PANTHERiPTHR13734:SF45. PTHR13734:SF45. 2 hits.
PfamiView protein in Pfam
PF03445. DUF294. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiView protein in SMART
SM00471. HDc. 1 hit.
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiView protein in PROSITE
PS51671. ACT. 2 hits.

Sequencei

Sequence statusi: Complete.

A0A1M2ZS16-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSRFDNLPH RRAIIDRRAL SDRVAALDIA DPGTLRRAVT AELKLALDAG
60 70 80 90 100
RAEIAQRLAE RPTRGLEAAN GQAFLIDQLI RVLFDAVTQR LYPLSNPTAA
110 120 130 140 150
ERLAILAVGG YGRGEMAPHS DVDIAFLTPW KQTPWAEQVI ESMLYALWDL
160 170 180 190 200
GLKVGHSSRS LDEMVRQAKS DVTIRTALLE ARYVWGDEAL ADEAALRFKA
210 220 230 240 250
EVQRGTERQF IADKLAERDQ RHIKMGDSRY VVEPNVKEGK GGMRDLHALF
260 270 280 290 300
WIGKYIYDVD RAAELVGKGL LTAAEYRQFH RADNFLWAVR CHLHLITGRA
310 320 330 340 350
EDRLTFDVQR EIAERMRFSE RPGRSGVERF MQYYFLQAKT VGTLTGVFIA
360 370 380 390 400
HLDEQFAARG RRFGLPTIRR RPRKLGGFVL DRGRLALPRD DFFAEDPVRL
410 420 430 440 450
IEIFQLADLH RLEIHPLAMR AANRDAKLVD DIRDDPRANA LFLDVLASPR
460 470 480 490 500
DPELVLRWMN EAGVFGRFVP DFGRVVAQMQ FDMYHHYTVD EHTIRAIGLL
510 520 530 540 550
SQIEHGEFSD EHPLSTAIFR QIVSRRALYA AVLLHDIAKG RGGDHSVLGA
560 570 580 590 600
QVAERLCPRF GLTPAETETV AWLVRYHLLM SATAFKRDLS DFKTILDFTA
610 620 630 640 650
VVQSPERLRM LLALTVVDIR AVGPGTWNGW KRQLLTDLYE AAEEVLRLGH
660 670 680 690 700
KQRGRSERLE QKQANLARSL GWDEKALRAY ARRLPEPYWI AEPEDVLAYN
710 720 730 740 750
ARFITAAGDA QLAIDAQIYP GRDATLVTVY AADHPGIFYR IAGAIHLAGG
760 770 780 790 800
NIIDARIHTT RDGMALDNFL VQDPLGRPFE EAGQLNRLRT AIEDALANRG
810 820 830 840 850
KLIDRLKAKA PPRVRADAFR IVPNVLVDNH ASNRFTVVEI NARDRPALLN
860 870 880 890 900
QLAHALFQSK VTIHSAHVAT YGERAVDTFY LTDLTGDKIT AGARVKALEK
910
RLLDAAAGVT AELAA
Length:915
Mass (Da):102,924
Last modified:March 15, 2017 - v1
Checksum:i074AC31D3EB7341B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
MKRR01000099 Genomic DNA. Translation: OJU19912.1.

Similar proteinsi

Entry informationi

Entry nameiA0A1M2ZS16_9SPHN
AccessioniPrimary (citable) accession number: A0A1M2ZS16
Entry historyiIntegrated into UniProtKB/TrEMBL: March 15, 2017
Last sequence update: March 15, 2017
Last modified: October 25, 2017
This is version 7 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteome, Multifunctional enzymeUniRule annotationSAAS annotation, Reference proteomeImported