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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Comamonadaceae bacterium CG2_30_57_122
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (pfp), ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15ATP; via amide nitrogenUniRule annotation1
Metal bindingi125Magnesium; catalyticUniRule annotation1
Sitei126Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei149Proton acceptorUniRule annotation1
Binding sitei184Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei244SubstrateUniRule annotation1
Binding sitei287Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi83 – 84ATPUniRule annotation2
Nucleotide bindingi124 – 127ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinaseUniRule annotationSAAS annotationImported, Transferase
Biological processGlycolysisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
ORF Names:AUK50_07585Imported
OrganismiComamonadaceae bacterium CG2_30_57_122Imported
Taxonomic identifieri1805089 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceae
Proteomesi
  • UP000182154 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 319PFKInterPro annotationAdd BLAST313

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni147 – 149Substrate bindingUniRule annotation3
Regioni191 – 193Substrate bindingUniRule annotation3
Regioni293 – 296Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III. 1 hit.
InterProiView protein in InterPro
IPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
IPR035966. PKF_sf.
PfamiView protein in Pfam
PF00365. PFK. 1 hit.
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
PROSITEiView protein in PROSITE
PS00433. PHOSPHOFRUCTOKINASE. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A1J5CER6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTPIKRIAV NTGGGDAPGL NAVIQSVMIA ADNRGWDCFG IRDGFNGILF
60 70 80 90 100
PERYAEGGVV RLTRERVRGI GHLGGTILGT TNKGNPLHFP VNLPDGSVRE
110 120 130 140 150
IDRSDEIITY FATHQIDALV SIGGDGSLTI ANALHQKGLR VVGVPKTIDN
160 170 180 190 200
DLDKTFTTFG FDTAVGFATE CLDRLHSTAE SHQRVMVVEV MGRYAGWIAL
210 220 230 240 250
HAGISGNAHA ILIPEIGFDI HKVAAKIRQR EAGGHLYSIV VVAEGAQASD
260 270 280 290 300
GHRELLAAAE VGHAERLGGM GEHVAEALAK ATGKEARVVV LGHLLRGGSP
310 320 330 340 350
TSFDRLAALR FGAAAVRALD AGHSGVMVSL GVNGVDYVDL EEVAGHIRNV
360
PMVCDTLQTG RDLGICFGD
Length:369
Mass (Da):38,969
Last modified:February 15, 2017 - v1
Checksum:i3BBF612FDB803362
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
MNXW01000177 Genomic DNA. Translation: OIP17334.1.

Similar proteinsi

Entry informationi

Entry nameiA0A1J5CER6_9BURK
AccessioniPrimary (citable) accession number: A0A1J5CER6
Entry historyiIntegrated into UniProtKB/TrEMBL: February 15, 2017
Last sequence update: February 15, 2017
Last modified: October 25, 2017
This is version 6 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteome, Reference proteomeImported