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Protein

Formamidopyrimidine-DNA glycosylase

Gene

mutM

Organism
Pseudomonas sp. OV546
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.UniRule annotationSAAS annotation

Catalytic activityi

Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.UniRule annotationSAAS annotation
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotationSAAS annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2Schiff-base intermediate with DNAUniRule annotation1
Active sitei3Proton donorUniRule annotation1
Active sitei58Proton donor; for beta-elimination activityUniRule annotation1
Binding sitei91DNAUniRule annotation1
Binding sitei110DNAUniRule annotation1
Binding sitei151DNAUniRule annotation1
Active sitei260Proton donor; for delta-elimination activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotationSAAS annotation, GlycosidaseUniRule annotationSAAS annotation, Hydrolase, LyaseUniRule annotationSAAS annotationImported, Multifunctional enzymeUniRule annotationSAAS annotation
Biological processDNA damage, DNA repairUniRule annotationSAAS annotation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Formamidopyrimidine-DNA glycosylaseUniRule annotation (EC:3.2.2.23UniRule annotation)
Short name:
Fapy-DNA glycosylaseUniRule annotation
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase MutMUniRule annotation (EC:4.2.99.18UniRule annotation)
Short name:
AP lyase MutMUniRule annotation
Gene namesi
Name:mutMUniRule annotation
Synonyms:fpgUniRule annotation
ORF Names:SAMN05428951_103262Imported
OrganismiPseudomonas sp. OV546Imported
Taxonomic identifieri1881063 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000199386 Componenti: Unassembled WGS sequence

Interactioni

Subunit structurei

Monomer.UniRule annotationSAAS annotation

Structurei

3D structure databases

SMRiA0A1I7I9V4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 113FPG_CATInterPro annotationAdd BLAST112
Domaini236 – 270FPG-typeInterPro annotationAdd BLAST35

Sequence similaritiesi

Belongs to the FPG family.UniRule annotationSAAS annotation

Keywords - Domaini

Zinc-fingerUniRule annotationSAAS annotation

Family and domain databases

Gene3Di3.20.190.10. 1 hit.
HAMAPiMF_00103. Fapy_DNA_glycosyl. 1 hit.
InterProiView protein in InterPro
IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR020629. Formamido-pyr_DNA_Glyclase.
IPR012319. FPG_cat.
IPR035937. MutM-like_N-ter.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010663. Znf_FPG/IleRS.
PfamiView protein in Pfam
PF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
SMARTiView protein in SMART
SM00898. Fapy_DNA_glyco. 1 hit.
SM01232. H2TH. 1 hit.
SUPFAMiSSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
TIGRFAMsiTIGR00577. fpg. 1 hit.
PROSITEiView protein in PROSITE
PS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A1I7I9V4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPELPEVETT RRGIAPHLEG QRVSRVVVRE RRLRWPIPED LDVRLSGQRI
60 70 80 90 100
VLVERRAKYL LINAEVGTLI SHLGMSGNLR LVEVGLPAAK HEHVDIELES
110 120 130 140 150
GMALRYTDPR RFGAMLWSQD PHNHELLLRL GPEPLTELFD GDRLFQLSRG
160 170 180 190 200
KSMAVKPFIM DNAVVVGVGN IYATEALFAA GIDPRRAAGG ISRGRYLKLA
210 220 230 240 250
IEIKRVLAAA IERGGTTLRD FIGGDGQPGY FQQELFVYGR GGEACKVCGT
260 270
ELRNVVLGQR ASVFCPRCQR
Length:270
Mass (Da):30,044
Last modified:November 22, 2017 - v1
Checksum:i19D3FE84BD930870
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FPCE01000003 Genomic DNA. Translation: SFU69765.1.
RefSeqiWP_015886368.1. NZ_FPCE01000003.1.

Similar proteinsi

Entry informationi

Entry nameiA0A1I7I9V4_9PSED
AccessioniPrimary (citable) accession number: A0A1I7I9V4
Entry historyiIntegrated into UniProtKB/TrEMBL: November 22, 2017
Last sequence update: November 22, 2017
Last modified: March 28, 2018
This is version 5 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported