Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Rhodopseudomonas pseudopalustris
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotationSAAS annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotation, Multifunctional enzymeUniRule annotationSAAS annotation, NucleotidyltransferaseUniRule annotationSAAS annotationImported, Transferase
LigandMagnesiumUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
Gene namesi
Name:glnDUniRule annotation
ORF Names:SAMN05444123_101475Imported
OrganismiRhodopseudomonas pseudopalustrisImported
Taxonomic identifieri1513892 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
Proteomesi
  • UP000199615 Componenti: Unassembled WGS sequence

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini500 – 623HDInterPro annotationAdd BLAST124
Domaini741 – 823ACTInterPro annotationAdd BLAST83
Domaini853 – 933ACTInterPro annotationAdd BLAST81

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 384UridylyltransferaseUniRule annotationAdd BLAST384

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili40 – 67Sequence analysisAdd BLAST28

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotationSAAS annotation

Keywords - Domaini

Coiled coilSequence analysis, RepeatSAAS annotation

Family and domain databases

HAMAPiMF_00277. PII_uridylyl_transf. 1 hit.
InterProiView protein in InterPro
IPR002912. ACT_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
IPR002934. Polymerase_NTP_transf_dom.
IPR010043. UTase/UR.
PANTHERiPTHR13734:SF45. PTHR13734:SF45. 2 hits.
PfamiView protein in Pfam
PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiView protein in SMART
SM00471. HDc. 1 hit.
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiView protein in PROSITE
PS51671. ACT. 2 hits.
PS51831. HD. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A1H8MDE6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKVATPPHR PAGDERFDTQ RITAEIAVLA AQHGGNDQAF RNALAQLMKA
60 70 80 90 100
ELAKARAEAE AQLLRDRHGR RCAERLCFVK DEIIRLLFMA ATKYLYNSPT
110 120 130 140 150
PSSSERMSVV ATGGYGRGLM APESDIDLLF ILPYKQTAWG EQVAEVVLYS
160 170 180 190 200
LWDIGLKVGH ATRSVDECIR QARADMTIRT AILETRFLTG DKALYAELVE
210 220 230 240 250
RFDKEVVEGT AAEFVTAKLA EREERHRRSG QSRYLVEPNV KDGKGGLRDL
260 270 280 290 300
HTLFWIAKYV YRVRETSELL ERGVFDAIEF RTFRRCEDFL WSVRCNLHFL
310 320 330 340 350
TRRAEERLSF DLQREIGVRL GYTSHPGMQD VERFMKHYFL IAKEVGNLTA
360 370 380 390 400
ILCAKLEDQQ AKPAPALSRM MARLRPGATR RRVPESDDFV IDNNRINLAA
410 420 430 440 450
PDVFKHDPVN LIRIFRLAQK NSLAFHPDAM RTVTRSLALI NAQLRDDPEA
460 470 480 490 500
NRLFIEILTS DNSEPVLRRM NETGVLGRFI RAFGRIVSMM QFNMYHSYTV
510 520 530 540 550
DEHLLRCVGN LQEIERGGND EFMLSSDLTR RIRPDHRAVL YVAVLLHDIA
560 570 580 590 600
KGQPEDHSTA GAKVARRLCP RFGFNAADTE LIAWLIEKHL VMSTVAQSRD
610 620 630 640 650
LSDRKTIENF AAVVESVEQM KLLTILTTAD IRGVGPGVWN GWKAQLLRTL
660 670 680 690 700
YYETEPVLTG GFSEVNRAER IRAAQAEFRA AFTEWPEAEL DAYVARHYPA
710 720 730 740 750
YWLKVELARK LRHARFLRAS EQAGNKLAVN VGFDEARGVT ELTILAVDHP
760 770 780 790 800
WLLSIIAGAC ASAGANIVDA QIYTTTDGRA LDTISIRREY DRDEDEGRRA
810 820 830 840 850
TRIGEIIEEV LEGKLRLPEA VARRATSSKT KLRAFVVEPE ISINNNWSDR
860 870 880 890 900
YTVIEVSGLD RPGLLYQLTT AISKLNLNIA SAHVATFGER ARDVFYVTDL
910 920 930
LGAQITAPTR QAAIKRALVH LLANGDAEQK PAA
Length:933
Mass (Da):105,371
Last modified:November 22, 2017 - v1
Checksum:i4FF353DD6CB3918B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FODT01000001 Genomic DNA. Translation: SEO15290.1.
RefSeqiWP_011500549.1. NZ_FODT01000001.1.

Similar proteinsi

Entry informationi

Entry nameiA0A1H8MDE6_9BRAD
AccessioniPrimary (citable) accession number: A0A1H8MDE6
Entry historyiIntegrated into UniProtKB/TrEMBL: November 22, 2017
Last sequence update: November 22, 2017
Last modified: January 31, 2018
This is version 3 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteomeImported