Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thymidylate synthase

Gene

thyA

Organism
Streptococcus sp. HMSC062H02
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotationSAAS annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei154PROSITE-ProRule annotation1
Active sitei154NucleophileUniRule annotation1
Binding sitei1815,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei189dUMPUniRule annotation1
Binding sitei2785,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi133 – 134dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi178 – 181dUMPUniRule annotation4
Nucleotide bindingi219 – 221dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferaseUniRule annotationSAAS annotation, Transferase
Biological processNucleotide biosynthesisUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotationSAAS annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
ORF Names:HMPREF2885_06165Imported
OrganismiStreptococcus sp. HMSC062H02Imported
Taxonomic identifieri1739389 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000176359 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 279Thymidylat_syntInterPro annotationAdd BLAST272

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiView protein in InterPro
IPR023451. Thymidate_synth/dCMP_Mease.
IPR036926. Thymidate_synth/dCMP_Mease_sf.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
PfamiView protein in Pfam
PF00303. Thymidylat_synt. 1 hit.
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
PROSITEiView protein in PROSITE
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A1F1EQT9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKADTIFKE NIERILKNGV FSEQARPKYK DGTVANSKYV TGAFAEYDLS
60 70 80 90 100
KGEFPITTLR PIAIKSAIKE VLWIYQDQSN SLEVLNNKYN VHYWNDWEVG
110 120 130 140 150
ETGTIGERYG AVVKKHDIIN KILKQLEANP WNRRNIISLW DYQAFEETDG
160 170 180 190 200
LLPCAFQTMF DVRRVDGEIY LDATLTQRSN DMLVAHHINA MQYVALQMMI
210 220 230 240 250
AKHFGWKVGK FFYFINNLHI YDNQFEQAQE LLRREPSNCQ PRLVLNVPDG
260 270
TNFFDIKAED FELVDYDPVK PQLKFDLAI
Length:279
Mass (Da):32,506
Last modified:February 15, 2017 - v1
Checksum:i7FAD2648C8F1FF56
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LTXM01000043 Genomic DNA. Translation: OFR44586.1.
RefSeqiWP_038804397.1. NZ_KV811129.1.

Similar proteinsi

Entry informationi

Entry nameiA0A1F1EQT9_9STRE
AccessioniPrimary (citable) accession number: A0A1F1EQT9
Entry historyiIntegrated into UniProtKB/TrEMBL: February 15, 2017
Last sequence update: February 15, 2017
Last modified: February 28, 2018
This is version 8 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteomeImported