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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Shewanella xiamenensis
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotationSAAS annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei564Aminoacyl-adenylateUniRule annotation1
Binding sitei608ATPUniRule annotation1
Metal bindingi903ZincUniRule annotation1
Metal bindingi906ZincUniRule annotation1
Metal bindingi923ZincUniRule annotation1
Metal bindingi926ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetaseUniRule annotationSAAS annotation, Ligase
Biological processProtein biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, ZincUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotationImported
ORF Names:ABT47_13095Imported
OrganismiShewanella xiamenensisImported
Taxonomic identifieri332186 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000094602 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Monomer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 643tRNA-synt_1InterPro annotationAdd BLAST616
Domaini688 – 844Anticodon_1InterPro annotationAdd BLAST157
Domaini901 – 928zf-FPG_IleRSInterPro annotationAdd BLAST28

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi58 – 68"HIGH" regionUniRule annotationAdd BLAST11
Motifi605 – 609"KMSKS" regionUniRule annotation5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotationSAAS annotation

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_FPG/IleRS.
PfamiView protein in Pfam
PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A1E3UXZ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDYKFTLNL PETEFPMRGN LANREPEMLE RWTKDGLYQQ IRDSRIGRTP
60 70 80 90 100
FILHDGPPYA NGSIHIGHSV NKILKDIIVK SKTMSGFDAP YVPGWDCHGL
110 120 130 140 150
PIELKVEQKV GKPGQKISAA EFREECRKYA AEQVDGQRED FIRLGVLGDW
160 170 180 190 200
QKPYLTMDFA TEANIVRSLS KVIENGHLHK GVKPVHWCTD CGSALAEAEV
210 220 230 240 250
EYEDKTSPAI DVAFTATDSK AVAAQFGVSD YSHPVAMVIW TTTPWTLPAN
260 270 280 290 300
RALSISPELD YSLVEFVKEG ATHAVILADV LVEACMTRYG AESHSVLGKV
310 320 330 340 350
KGAALELVRF KHPFLAFDVP AILGDHVTTD AGTGVVHTAP GHGQDDFVVG
360 370 380 390 400
QKYGLEVANP VGDNGVYKPE TEFFAGQHVF KANDNVVALL KEKGALLHHV
410 420 430 440 450
AYRHSYPHCW RHKTPIIFRA TPQWFISMDN HNLRKQALSE IEQTQWIPDW
460 470 480 490 500
GQSRIEKMVE NRPDWCISRQ RTWGVPITLF VHRETEELHP DSVSLMARVA
510 520 530 540 550
NRIEQEGIQA WWDLDAAELL GEEAEQYRKV TDTLDVWYDS GSTFASVVAA
560 570 580 590 600
RPEFQGHGVD LYLEGSDQHR GWFMSSLMIS TAMTGEAPYK QVLTHGFTVD
610 620 630 640 650
GKGRKMSKSI GNVIAPQQVT NKLGADILRL WVAATDYSGE MTVSDEILNR
660 670 680 690 700
SADAYRRIRN TARFLLANLN GFDPAKDLVA VEDMVALDRW AVRRAAALQQ
710 720 730 740 750
EIIEAYEQYN FHIVTQKLMQ FCSVELGSFY LDIIKDRQYT AKQEGHARRS
760 770 780 790 800
CQSALFHIAE AMVRWIAPIL SFTADEVWQL LPGQRDAYVF TQEWYQGLQS
810 820 830 840 850
ITLDTDLSDA YWENLLTVRN EVNKVIEQAR RDKRIGGSLE AEVTLFADAA
860 870 880 890 900
LTEQLTHIGD ELRFVLLTSE AKVLPLADAT SDAVETELAS LKLVVNATTA
910 920 930 940
EKCERCWHHR EEVGTIEAHP TLCHRCVTNI EGDGEVRLFA
Length:940
Mass (Da):105,639
Last modified:January 18, 2017 - v1
Checksum:i488A1E76F30FFB7A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LDOA01000001 Genomic DNA. Translation: ODR86173.1.
RefSeqiWP_069454416.1. NZ_LDOA01000001.1.

Genome annotation databases

EnsemblBacteriaiODR86173; ODR86173; ABT47_13095.
PATRICifig|332186.8.peg.2710.

Similar proteinsi

Entry informationi

Entry nameiA0A1E3UXZ4_9GAMM
AccessioniPrimary (citable) accession number: A0A1E3UXZ4
Entry historyiIntegrated into UniProtKB/TrEMBL: January 18, 2017
Last sequence update: January 18, 2017
Last modified: November 22, 2017
This is version 9 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteomeImported