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Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Geobacillus thermoleovorans (Bacillus thermoleovorans)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi150Zinc 1UniRule annotation1
Metal bindingi153Zinc 1UniRule annotation1
Metal bindingi167Zinc 2UniRule annotation1
Metal bindingi170Zinc 2UniRule annotation1
Metal bindingi193Zinc 2UniRule annotation1
Metal bindingi196Zinc 2UniRule annotation1
Metal bindingi207Zinc 1UniRule annotation1
Metal bindingi210Zinc 1UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri137 – 219CR-typePROSITE-ProRule annotationAdd BLAST83

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperoneUniRule annotationSAAS annotation
Biological processDNA replicationUniRule annotation, Stress responseUniRule annotation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJUniRule annotation
Gene namesi
Name:dnaJUniRule annotation
ORF Names:BGM21_12320Imported
OrganismiGeobacillus thermoleovorans (Bacillus thermoleovorans)Imported
Taxonomic identifieri33941 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillusGeobacillus thermoleovorans group
Proteomesi
  • UP000094325 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

GO - Molecular functioni

Structurei

3D structure databases

SMRiA0A1D7NDG9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 69JInterPro annotationAdd BLAST65
Domaini137 – 219CR-typeInterPro annotationAdd BLAST83
Repeati150 – 157CXXCXGXG motifUniRule annotation8
Repeati167 – 174CXXCXGXG motifUniRule annotation8
Repeati193 – 200CXXCXGXG motifUniRule annotation8
Repeati207 – 214CXXCXGXG motifUniRule annotation8

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri137 – 219CR-typePROSITE-ProRule annotationAdd BLAST83

Keywords - Domaini

RepeatUniRule annotationSAAS annotation, Zinc-fingerUniRule annotationSAAS annotation

Phylogenomic databases

KOiK03686.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
cd10719. DnaJ_zf. 1 hit.
Gene3Di1.10.287.110. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiView protein in InterPro
IPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
IPR036410. HSP_DnaJ_Cys-rich_dom_sf.
IPR036869. J_dom_sf.
PfamiView protein in Pfam
PF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
PRINTSiPR00625. JDOMAIN.
SMARTiView protein in SMART
SM00271. DnaJ. 1 hit.
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsiTIGR02349. DnaJ_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A1D7NDG9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKRDYYEIL GVSKNATKDE IKKAYRKLSK QYHPDVNKAP DAAEKFKEIK
60 70 80 90 100
EAYEVLSDDE KRARYDRFGH ADPNETFGGG GFQGGGFDFG GFSGFGGFED
110 120 130 140 150
IFETFFGAGP RRRASGPRKG ADVEYMMTLT FEEAAFGKET EIEIPREETC
160 170 180 190 200
DTCQGSGAKP GTSPTSCPHC HGSGQVTSEQ ATPFGRIVNR RTCPVCGGTG
210 220 230 240 250
RYIPEKCPTC GGTGRVKRRK KIHVKIPAGV DDGQQLRVAG QGEPGVNGGP
260 270 280 290 300
PGDLYIIFRV EPHEFFKRDG DDIYCEVPLS FAQAALGDEI EVPTLHGHVK
310 320 330 340 350
LKIPAGTQTG TRFRLKGKGV PNVRGYGQGD QHVIVRVVTP TKLTEKQKQL
360 370 380
LREFERLGGD TMHDGPHGRF FEKVKKAFKG EA
Length:382
Mass (Da):41,928
Last modified:January 18, 2017 - v1
Checksum:i3B36FFCB391A306F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP017071 Genomic DNA. Translation: AOL35227.1.
RefSeqiWP_011231982.1. NZ_CP017071.1.

Genome annotation databases

GeneIDi32064384.
KEGGigtk:GT3570_12145.

Similar proteinsi

Entry informationi

Entry nameiA0A1D7NDG9_GEOTH
AccessioniPrimary (citable) accession number: A0A1D7NDG9
Entry historyiIntegrated into UniProtKB/TrEMBL: January 18, 2017
Last sequence update: January 18, 2017
Last modified: March 28, 2018
This is version 11 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteomeImported