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Protein

Fumarate hydratase class II

Gene

aspA

Organism
Mycobacterium peregrinum
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (aspA), Fumarate hydratase class II (aspA)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei187SubstrateUniRule annotation1
Active sitei188Proton donor/acceptorUniRule annotation1
Active sitei319UniRule annotation1
Binding sitei320SubstrateUniRule annotation1
Sitei332Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotationImported
Biological processTricarboxylic acid cycleUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:aspAImported
Synonyms:fumCUniRule annotation
ORF Names:A5792_17935Imported
OrganismiMycobacterium peregrinumImported
Taxonomic identifieri43304 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000093902 Componenti: Unassembled WGS sequence

Subcellular locationi

A0A1A0R846:
  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 343Lyase_1InterPro annotationAdd BLAST325
Domaini409 – 465FumaraseC_CInterPro annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni105 – 107Substrate bindingUniRule annotation3
Regioni129 – 132Substrate binding (B site)UniRule annotation4
Regioni139 – 141Substrate bindingUniRule annotation3
Regioni325 – 327Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Family and domain databases

CDDicd01362. Fumarase_classII. 1 hit.
Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC. 1 hit.
InterProiView protein in InterPro
IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiView protein in Pfam
PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
PROSITEiView protein in PROSITE
PS00163. FUMARATE_LYASES. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A1A0R846-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTDTDGAVE YRIEHDTMGE VRVPVNALWR AQTQRAVENF PISFRGLERT
60 70 80 90 100
QIRALGLLKA ACAQVNKDLG LLAAEKADAI IAAAGEIADG LHDDQFPIDV
110 120 130 140 150
FQTGSGTSSN MNANEVIASI AARNGVTVHP NDDVNMSQSS NDTFPTATHI
160 170 180 190 200
AATEAAVRHL IPALEVLHES LAAKAKQWKT VVKSGRTHLM DAVPVTLGQE
210 220 230 240 250
FGGYARQIEA GIERVKATLP RLGELAIGGT AVGTGLNAPD GFGPKVVEVL
260 270 280 290 300
VNQTGLAELR TAADSFEAQA ARDGLVEASG ALKTIAASLT KIANDVRWMG
310 320 330 340 350
SGPLTGLGEI QLPDLQPGSS IMPGKVNPVL PEAVTQVAAQ VIGNDAAVTV
360 370 380 390 400
GGLSGAFELN VYIPMMARNV LESFTLLSNV SRLFAEKCID GLVANEEHLR
410 420 430 440 450
TLAESSPSIV TPLNSAIGYE EAAKVAKQAL KEKKTIRQTV IDRGLIGDKL
460 470
SEAELDKRLD VLAMAKVVDE RLREEQ
Length:476
Mass (Da):50,428
Last modified:October 5, 2016 - v1
Checksum:iC0B43CA3D48724F3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LZSO01000017 Genomic DNA. Translation: OBB30641.1.
RefSeqiWP_064931935.1. NZ_LZSO01000017.1.

Genome annotation databases

EnsemblBacteriaiOBB30641; OBB30641; A5792_17935.

Similar proteinsi

Entry informationi

Entry nameiA0A1A0R846_MYCPR
AccessioniPrimary (citable) accession number: A0A1A0R846
Entry historyiIntegrated into UniProtKB/TrEMBL: October 5, 2016
Last sequence update: October 5, 2016
Last modified: September 27, 2017
This is version 9 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteomeImported