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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Oleiphilus sp. HI0069
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF)
  2. Phosphoribosylamine--glycine ligase (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2For GATase activityUniRule annotation1
Active sitei2NucleophileUniRule annotation1
Metal bindingi303MagnesiumUniRule annotation1
Metal bindingi365MagnesiumUniRule annotation1
Metal bindingi366MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferaseUniRule annotationImported, Transferase
Biological processPurine biosynthesisUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Short name:
GPATaseUniRule annotation
Gene namesi
Name:purFUniRule annotation
ORF Names:A3741_11075Imported
OrganismiOleiphilus sp. HI0069Imported
Taxonomic identifieri1822245 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesOleiphilaceaeOleiphilus
Proteomesi
  • UP000077151 Componenti: Unassembled WGS sequence

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 234Glutamine amidotransferase type-2InterPro annotationAdd BLAST233

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferaseUniRule annotation

Family and domain databases

CDDicd00715. GPATase_N. 1 hit.
cd06223. PRTases_typeI. 1 hit.
Gene3Di3.60.20.10. 2 hits.
HAMAPiMF_01931. PurF. 1 hit.
InterProiView protein in InterPro
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
IPR035584. PurF_N.
PfamiView protein in Pfam
PF00156. Pribosyltran. 1 hit.
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiView protein in PROSITE
PS51278. GATASE_TYPE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A176H116-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIVGVVGK NNANQSLYDA LTVLQHRGQD AAGIVTCQDD RFFLRKDNGL
60 70 80 90 100
VRDVFRTRHM KRLQGSVGIG HVRYPTAGSS SSAEAQPFYV NSPYGITLAH
110 120 130 140 150
NGNLTNTAEL ASGVLKSDLR HVNTNSDSEV LLNVFAHELQ EQGKLVPTQV
160 170 180 190 200
EIFNAVKEVH KRCRGAYAVI AMITGYGVLA FRDPYGIRPV CYGKRETEQG
210 220 230 240 250
TEYMISSESV ALAALGFELV RDIEPGEAIY IENDGTLHTQ QCSENPKLTP
260 270 280 290 300
CIFEHVYFAR PDSIIDKVSV YKARLRMGET LAQKVKRLRP DHDIDVVMPI
310 320 330 340 350
PDTSRTSAMQ MAVELGVKFR EGFIKNRYIG RTFIMPGQNQ RKKSVRQKLN
360 370 380 390 400
PIELEFKDKN VMLVDDSIVR GTTCREIVQM ARDAGAKNVY FASAAPEVRY
410 420 430 440 450
PNVYGIDMPA ATELIAHDRT VEDIQEIIGA DWLVYQDLED LIRCAKDGNE
460 470 480 490 500
DIDEFDCSVF NGVYPTGDIC GDYLDRLEQA RNDSNKKKLN EDDATIDLHN

DN
Length:502
Mass (Da):56,140
Last modified:September 7, 2016 - v1
Checksum:i9CFC7DD1855D0EF2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LWFK01002062 Genomic DNA. Translation: KZY76283.1.

Genome annotation databases

EnsemblBacteriaiKZY76283; KZY76283; A3741_11075.

Similar proteinsi

Entry informationi

Entry nameiA0A176H116_9GAMM
AccessioniPrimary (citable) accession number: A0A176H116
Entry historyiIntegrated into UniProtKB/TrEMBL: September 7, 2016
Last sequence update: September 7, 2016
Last modified: October 25, 2017
This is version 12 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteome, Reference proteomeImported