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Protein

Malate synthase G

Gene

glcB

Organism
Corynebacterium crudilactis
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotationSAAS annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei140Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Binding sitei297Acetyl-CoAUniRule annotation1
Binding sitei334Acetyl-CoAUniRule annotation1
Active sitei361Proton acceptorUniRule annotation1
Binding sitei361GlyoxylateUniRule annotation1
Metal bindingi452MagnesiumUniRule annotation1
Binding sitei452GlyoxylateUniRule annotation1
Metal bindingi480MagnesiumUniRule annotation1
Binding sitei561Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Active sitei652Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotationSAAS annotation
Biological processGlyoxylate bypassUniRule annotationSAAS annotation, Tricarboxylic acid cycleUniRule annotationSAAS annotation
LigandMagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
ORF Names:ccrud_10335Imported
OrganismiCorynebacterium crudilactisImported
Taxonomic identifieri1652495 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000076929 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei638Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

OxidationUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni147 – 148Acetyl-CoA bindingUniRule annotation2
Regioni477 – 480Glyoxylate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotationSAAS annotation

Phylogenomic databases

KOiK01638.

Family and domain databases

HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiView protein in InterPro
IPR011076. Malate_synth-like_sf.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
PfamiView protein in Pfam
PF01274. Malate_synthase. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A172QV25-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEQQKLSAQ TADNAGTAGL TDSTVRVEVG GMQVAQVLYD FVTEAVLPRV
60 70 80 90 100
GVDAEKFWSG FASIARDLTP RNRELLARRD ELQALIDDYH RNNSGTAIDQ
110 120 130 140 150
DAYEEFLKEI GYLVEPPEAA EIRTQNIDKE ISSTAGPQLV VPILNARFAL
160 170 180 190 200
NAANARWGSL YDALYGTNAI PETDGAEKGN EYNPIRGQKV IEWGREFLDN
210 220 230 240 250
VVPLDGASHA DVDKYNISDG KLAAHIGDDV YRLKNRDAYR GFTGNFLDPE
260 270 280 290 300
AILLETNGLH IELQIDPVHP IGKADKTGLK DILLESAITT IMDFEDSVAA
310 320 330 340 350
VDAEDKTLGY TNWFGLNTGE LNEEVSKNGR SFTRELNSDR VYIGRNGTEL
360 370 380 390 400
VLHGRSLLFV RNVGHLMQNP SILIDGEEIF EGIMDAVITS VCAIPGIAPQ
410 420 430 440 450
NKKRNSRKGS IYIVKPKQHG PEEVAFTNEL FGRVEDLLEL PRYTLKVGVM
460 470 480 490 500
DEERRTSVNL DACIMEVADR LAFINTGFLD RTGDEIHTSM EAGAMVRKAD
510 520 530 540 550
MQTAPWKQSY EDNNVDAGIQ RGLPGKAQIG KGMWAMTELM AEMLEKKIGQ
560 570 580 590 600
PREGANTAWV PSPTGATLHA THYHLVDVFA VQAELRVAGR RDTLRNILTI
610 620 630 640 650
PLALNAEWSA EEKKEELDNN CQSILGYVVR WVEQGVGCSK VPDIHNIALM
660 670 680 690 700
EDRATLRISS QMLANWIRHD VVSEEQVVES LERMAVVVDK QNAGDDAYRD
710 720 730 740
MAPNYDASIA FQAAKQLIFE GTKSPAGYTE PILHARRREF KAQN
Length:744
Mass (Da):82,529
Last modified:September 7, 2016 - v1
Checksum:i9FA5C93F1297D2D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP015622 Genomic DNA. Translation: ANE04559.1.
RefSeqiWP_066567172.1. NZ_CP015622.1.

Genome annotation databases

EnsemblBacteriaiANE04559; ANE04559; ccrud_10335.
KEGGiccjz:ccrud_10335.

Similar proteinsi

Entry informationi

Entry nameiA0A172QV25_9CORY
AccessioniPrimary (citable) accession number: A0A172QV25
Entry historyiIntegrated into UniProtKB/TrEMBL: September 7, 2016
Last sequence update: September 7, 2016
Last modified: November 22, 2017
This is version 12 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteomeImported