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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Shewanella baltica
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotationSAAS annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei564Aminoacyl-adenylateUniRule annotation1
Binding sitei608ATPUniRule annotation1
Metal bindingi903ZincUniRule annotation1
Metal bindingi906ZincUniRule annotation1
Metal bindingi923ZincUniRule annotation1
Metal bindingi926ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetaseUniRule annotationSAAS annotation, Ligase
Biological processProtein biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, ZincUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotationImported
ORF Names:A1L58_16245Imported
OrganismiShewanella balticaImported
Taxonomic identifieri62322 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000076650 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Monomer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 643tRNA-synt_1InterPro annotationAdd BLAST616
Domaini688 – 844Anticodon_1InterPro annotationAdd BLAST157
Domaini901 – 928zf-FPG_IleRSInterPro annotationAdd BLAST28

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi58 – 68"HIGH" regionUniRule annotationAdd BLAST11
Motifi605 – 609"KMSKS" regionUniRule annotation5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotationSAAS annotation

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_FPG/IleRS.
PfamiView protein in Pfam
PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A165JWY2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDYKFTLNL PETEFPMRGN LANREPEMLE RWTKDGLYQQ IRDSRIGRTP
60 70 80 90 100
FILHDGPPYA NGSIHIGHSV NKILKDIIIK SKTMSGFDAP YVPGWDCHGL
110 120 130 140 150
PIELKVEQKV GKPGQKISAA EFREECRKYA AEQVNGQRED FIRLGVLGDW
160 170 180 190 200
QNPYLTMDFS TEANIVRSLS KVIESGHLHK GVKPVHWCTD CGSALAEAEV
210 220 230 240 250
EYEDKTSPAI DVAFVAADSK AVAAKFGVSD YLHPVSMVIW TTTPWTLPAN
260 270 280 290 300
RALSLSPELD YSLVEFEKDG VTQALILAEV LVESCLTRYN VESHTVLGSA
310 320 330 340 350
KGAAFELVRF NHPFLDFDVP AILGDHVTTD AGTGIVHTAP GHGQDDFVVG
360 370 380 390 400
QKYGLEVANP VGDNGVYKPD TEYFAGQHVF KANDNVVALL REKSALLNHV
410 420 430 440 450
AYRHSYPHCW RHKTPIIFRA TPQWFISMDN HGLRTQALKE IEQTQWIPDW
460 470 480 490 500
GQSRIEKMVE NRPDWCISRQ RTWGVPITLF VNRETEELHP DSVSLMERVA
510 520 530 540 550
SRIEQQGIQA WWDLDAAELL GDEAEQYRKV TDTLDVWFDS GSTFSSVVAA
560 570 580 590 600
RPEFHGHGVD LYLEGSDQHR GWFMSSLMIS TAMNGKAPYK QVLTHGFTVD
610 620 630 640 650
GKGRKMSKSI GNVIAPQTVT NKLGADILRL WVAATDYSGE MTVSDEILNR
660 670 680 690 700
SADAYRRIRN TARFLLANLN GFEPAKDLVA VEDMVALDRW VVRRAAALQQ
710 720 730 740 750
EIIEAYEQYN FHIVTQKLMQ FCSVELGSFY LDIIKDRQYT AKQEGHARRS
760 770 780 790 800
CQSALYLISE AMVRWIAPIL SFTADEVWQL LPGERDAYVF TQEWYQGLKS
810 820 830 840 850
VTLTTDLSDD YWQQLLTVRN EVNKVIEQAR RDKRIGGSLE AEVTLFADAA
860 870 880 890 900
LTEQLTHIGD ELRFVLLTSE AKVLPLADAT SEAVETELAS LKLLVASSTA
910 920 930 940
EKCERCWHHR EEVGTIEAHP TLCTRCVTNI EGDGEVRLFA
Length:940
Mass (Da):105,818
Last modified:July 6, 2016 - v1
Checksum:i8C47B219FD1079AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LWED01000021 Genomic DNA. Translation: KZK69255.1.
RefSeqiWP_063883427.1. NZ_LWED01000021.1.

Genome annotation databases

EnsemblBacteriaiKZK69255; KZK69255; A1L58_16245.

Similar proteinsi

Entry informationi

Entry nameiA0A165JWY2_9GAMM
AccessioniPrimary (citable) accession number: A0A165JWY2
Entry historyiIntegrated into UniProtKB/TrEMBL: July 6, 2016
Last sequence update: July 6, 2016
Last modified: November 22, 2017
This is version 11 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteomeImported