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Protein

ATPase GET3

Gene

get3

Organism
Didymella rabiei (Chickpea ascochyta blight fungus) (Mycosphaerella rabiei)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting.UniRule annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei67UniRule annotation1
Binding sitei250ATPUniRule annotation1
Binding sitei277ATPUniRule annotation1
Metal bindingi288Zinc; shared with dimeric partnerUniRule annotation1
Metal bindingi291Zinc; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 45ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotation
Biological processTransportUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, ZincUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase GET3UniRule annotation (EC:3.6.-.-UniRule annotation)
Alternative name(s):
Arsenical pump-driving ATPaseUniRule annotation
Arsenite-stimulated ATPaseUniRule annotation
Golgi to ER traffic protein 3UniRule annotation
Guided entry of tail-anchored proteins 3UniRule annotation
Gene namesi
Name:get3Imported
Synonyms:GET3UniRule annotation
ORF Names:ST47_g703Imported
OrganismiDidymella rabiei (Chickpea ascochyta blight fungus) (Mycosphaerella rabiei)Imported
Taxonomic identifieri5454 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaDothideomycetesPleosporomycetidaePleosporalesPleosporineaeDidymellaceaeAscochyta
Proteomesi
  • UP000076837 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation, Endoplasmic reticulumUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

GO - Molecular functioni

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 338ArsA_ATPaseInterPro annotationAdd BLAST308

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili214 – 234Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the arsA ATPase family.UniRule annotationSAAS annotation

Keywords - Domaini

Coiled coilSequence analysis

Family and domain databases

CDDicd02035 ArsA, 1 hit
HAMAPiMF_03112 Asna1_Get3, 1 hit
InterProiView protein in InterPro
IPR025723 Anion-transp_ATPase-like_dom
IPR016300 ATPase_ArsA/GET3
IPR027542 ATPase_ArsA/GET3_euk
IPR027417 P-loop_NTPase
PANTHERiPTHR10803 PTHR10803, 1 hit
PTHR10803:SF3 PTHR10803:SF3, 1 hit
PfamiView protein in Pfam
PF02374 ArsA_ATPase, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00345 GET3_arsA_TRC40, 1 hit

Sequencei

Sequence statusi: Complete.

A0A163LUT0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATTAALQKL QNPPEDLEPT LQSILDQKSL RWIFVGGKGG VGKTTTSCSL
60 70 80 90 100
AIQLAKHRKS VLLISTDPAH NLSDAFNQKF GKDARLVNGF DNLSAMEIDP
110 120 130 140 150
NGSIQDLLAA GGEEGQDPMA GLGGMGNMMQ DLAFSIPGVD EAMSFAEVLK
160 170 180 190 200
QVKSMSYETI IFDTAPTGHT LRFLQFPTVM EKALGKVSQL SRQFGPMLNG
210 220 230 240 250
FLGGGGRLPN GQSMDELVEK MDALQKTIAE VNGQFKDADL TTFVCVCIPE
260 270 280 290 300
FLSLYETERM IQELNSYEID THSIVVNQLL FPKQDNPCEQ CNARRKMQKK
310 320 330 340
YLDQIEELYD EFNVVKMPLL VEEVRGKEKL ENFSKMLVEP FVPPQ
Length:345
Mass (Da):38,208
Last modified:July 6, 2016 - v1
Checksum:iFF5EDBE10E1E6215
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JYNV01000032 Genomic DNA Translation: KZM28140.1

Genome annotation databases

EnsemblFungiiKZM28140; KZM28140; ST47_g703

Similar proteinsi

Entry informationi

Entry nameiA0A163LUT0_DIDRA
AccessioniPrimary (citable) accession number: A0A163LUT0
Entry historyiIntegrated into UniProtKB/TrEMBL: July 6, 2016
Last sequence update: July 6, 2016
Last modified: May 23, 2018
This is version 15 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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