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Protein

Carbamoyl-phosphate synthase large chain

Gene

carB

Organism
Lactobacillus plantarum
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+SAAS annotation
  • Mn2+SAAS annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes carbamoyl phosphate from bicarbonate.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase large chain (pyrAB), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase small chain (pyrAa2), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase large chain (pyrAB), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase small chain (carA)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from bicarbonate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.UniRule annotationSAAS annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase large chain (pyrAB), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase small chain (pyrAa2), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase large chain (pyrAB), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase small chain (carA)
  2. Aspartate carbamoyltransferase (pyrB), Aspartate carbamoyltransferase (pyrB), Aspartate carbamoyltransferase (pyrB), Aspartate carbamoyltransferase (pyrB), Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (ura4), Dihydroorotase (pyrC), Dihydroorotase (pyrC), Dihydroorotase (pyrC), Dihydroorotase (pyrC), Dihydroorotase (pyrC), Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationSAAS annotation
Biological processAmino-acid biosynthesis, Arginine biosynthesisUniRule annotationSAAS annotation, Pyrimidine biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, MagnesiumSAAS annotation, ManganeseSAAS annotation, Metal-bindingSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase large chainUniRule annotation (EC:6.3.5.5UniRule annotation)
Alternative name(s):
Carbamoyl-phosphate synthetase ammonia chainUniRule annotation
Gene namesi
Name:carBUniRule annotation
ORF Names:Nizo2891_0277Imported
OrganismiLactobacillus plantarumImported
Taxonomic identifieri1590 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
Proteomesi
  • UP000076745 Componenti: Unassembled WGS sequence

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini133 – 327ATP-graspInterPro annotationAdd BLAST195
Domaini671 – 861ATP-graspInterPro annotationAdd BLAST191

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 401Carboxyphosphate synthetic domainUniRule annotationAdd BLAST401
Regioni547 – 929Carbamoyl phosphate synthetic domainUniRule annotationAdd BLAST383
Regioni930 – 1058Allosteric domainUniRule annotationAdd BLAST129

Sequence similaritiesi

Belongs to the CarB family.UniRule annotationSAAS annotation

Keywords - Domaini

RepeatUniRule annotationSAAS annotation

Family and domain databases

CDDicd01424. MGS_CPS_II. 1 hit.
Gene3Di1.10.1030.10. 1 hit.
3.30.1490.20. 1 hit.
3.40.50.1380. 1 hit.
HAMAPiMF_01210_B. CPSase_L_chain_B. 1 hit.
InterProiView protein in InterPro
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR006275. CarbamoylP_synth_lsu.
IPR005480. CarbamoylP_synth_lsu_oligo.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR005483. CbamoylP_synth_lsu_CPSase_dom.
IPR011607. MGS-like_dom.
IPR033937. MGS_CPS_CarB.
IPR016185. PreATP-grasp_dom.
PfamiView protein in Pfam
PF02786. CPSase_L_D2. 2 hits.
PF02787. CPSase_L_D3. 1 hit.
PF02142. MGS. 1 hit.
PRINTSiPR00098. CPSASE.
SMARTiView protein in SMART
SM01096. CPSase_L_D3. 1 hit.
SM00851. MGS. 1 hit.
SUPFAMiSSF48108. SSF48108. 1 hit.
SSF52335. SSF52335. 1 hit.
SSF52440. SSF52440. 2 hits.
TIGRFAMsiTIGR01369. CPSaseII_lrg. 1 hit.
PROSITEiView protein in PROSITE
PS50975. ATP_GRASP. 2 hits.
PS00866. CPSASE_1. 2 hits.
PS00867. CPSASE_2. 2 hits.

Sequencei

Sequence statusi: Complete.

A0A162GDH0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKRTDIHKI MVIGSGPIII GQAAEFDYSG TQACLALKEL DYEVVLVNSN
60 70 80 90 100
PATIMTDKEI ADQVYLEPIT LEFVSQILRK EHPDAILPTL GGQQGLNMAM
110 120 130 140 150
ELSKSGILDE LHIELLGTKL SAIDQAEDRE QFKALMEELG EPVPASGIAR
160 170 180 190 200
TVDEALAFAE QAGYPVIVRP AFTMGGTGGG IAETPQQLHD ITENGLALSP
210 220 230 240 250
VTQVLIEQSI AGYKEIEFEV MRDAADNAMV VCNMENFDPV GIHTGDSIVY
260 270 280 290 300
APVQTLADRE VQLLRDAALK IIRALKIEGG CNVQLALDPN SFNYYIIEVN
310 320 330 340 350
PRVSRSSALA SKATGYPIAK MAAKIAVGLH LDEIKNPVTG TTYAEFEPAL
360 370 380 390 400
DYVVCKIPRW PFDKFTHADR RLGTQMKATG EVMAIGRNIE EATLKAVRSL
410 420 430 440 450
EIGVHHVEEP ALRSVDDDVL SDKLIHAQDD RLFYLTEAIR RGYPIDELAE
460 470 480 490 500
LTKINVFFLD KLLQIIEIEQ ALRTHTDDIE TLTVAKRNGF ADQTVADYWH
510 520 530 540 550
ETIDQVRDFR LAHKLAPVYK MVDTCAGEFA SETPYYYGTY EFENESIVTK
560 570 580 590 600
RPSVLVLGSG PIRIGQGVEF DYATVHSVKA IQKAGYEAII MNSNPETVST
610 620 630 640 650
DFSVSDKLYF EPLTIEDVLN VIELEKPVGV IVQFGGQTAI NLAKPLADHG
660 670 680 690 700
IKILGTSVAD VNRAEDRDEF DKVIKALAIP QPAGDTASDE ATALAIADKL
710 720 730 740 750
GYPVLVRPSY VLGGRAMEIV KKRTDLDYYM HNAVKVSHDH PVLVDSYLVG
760 770 780 790 800
KECEVDAICD GQTVLIPGIM EHIERAGVHS GDSMAVYPPQ SLSAAVQAQI
810 820 830 840 850
VDYTEKLAIA LNCVGMMNIQ FVIHDDQVYV IEVNPRASRT VPFLSKVTNI
860 870 880 890 900
PMAQVATRAI LGQSLAEQGY QTGLVTPGPL VHVKAPVFSF SKLNRVDSLL
910 920 930 940 950
GPEMKSTGEV MGSDVTMAKA LYKAFEAAKL HVPSHGNVLL TVRDEDKPET
960 970 980 990 1000
VALAKRFHAL GYQLLATRGT ATALTTHGLP VTTVDKIDSG EHDLLHRMEA
1010 1020 1030 1040 1050
GEIQVVINTV SDEEQAENDG TLIRNTSIMH GIPLFTALDT VAAILQVRES

QSFVTQAL
Length:1,058
Mass (Da):115,672
Last modified:July 6, 2016 - v1
Checksum:iE5777BE9ECEC78F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LUXG01000005 Genomic DNA. Translation: KZU81240.1.
RefSeqiWP_063488179.1. NZ_LUXG01000005.1.

Genome annotation databases

EnsemblBacteriaiKZU81240; KZU81240; Nizo2891_0277.
PATRICifig|1590.207.peg.1310.

Similar proteinsi

Entry informationi

Entry nameiA0A162GDH0_LACPN
AccessioniPrimary (citable) accession number: A0A162GDH0
Entry historyiIntegrated into UniProtKB/TrEMBL: July 6, 2016
Last sequence update: July 6, 2016
Last modified: September 27, 2017
This is version 11 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteomeImported