Outer capsid protein VP4

Functionⁱ

Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm. In integrin-dependent strains, VP5* targets the integrin heterodimer ITGA2/ITGB1 for cell attachment.SAAS annotation

VP8* forms the head of the spikes. It is the viral hemagglutinin and an important target of neutralizing antibodies. In sialic acid-dependent strains, VP8* binds to host cell sialic acid, most probably a ganglioside, providing the initial contact.SAAS annotation

GO - Biological processⁱ

permeabilization of host organelle membrane involved in viral entry into host cell Source: UniProtKB-KW
viral entry via permeabilization of inner membrane Source: UniProtKB-KW
virion attachment to host cell Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	HemagglutininSAAS annotation
Biological process	Host-virus interaction, Viral attachment to host cellSAAS annotation, Viral penetration into host cytoplasm, Viral penetration via permeabilization of host membraneSAAS annotation, Virus entry into host cell

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Outer capsid protein VP4UniRule annotationSAAS annotation
Organismⁱ	Rotavirus G3Imported
Taxonomic identifierⁱ	73036 [NCBI]
Taxonomic lineageⁱ	Viruses › dsRNA viruses › Reoviridae › Sedoreovirinae › Rotavirus › Rotavirus A

Subcellular locationⁱ

GO - Cellular componentⁱ

host cell rough endoplasmic reticulum Source: UniProtKB-SubCell
viral outer capsid Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Capsid protein, Host endoplasmic reticulumSAAS annotation, Outer capsid proteinSAAS annotation, Virion

Interactionⁱ

Subunit structureⁱ

VP4 is a homotrimer (Potential). VP4 adopts a dimeric appearance above the capsid surface, while forming a trimeric base anchored inside the capsid layer. Only hints of the third molecule are observed above the capsid surface. It probably performs a series of molecular rearrangements during viral entry. Prior to trypsin cleavage, it is flexible. The priming trypsin cleavage triggers its rearrangement into rigid spikes with approximate two-fold symmetry of their protruding parts. After an unknown second triggering event, cleaved VP4 may undergo another rearrangement, in which two VP5* subunits fold back on themselves and join a third subunit to form a tightly associated trimer, shaped like a folded umbrella. VP5* is a homotrimer (Potential). The trimer is coiled-coil stabilized by its C-terminus, however, its N-terminus, known as antigen domain or "body", seems to be flexible allowing it to self-associate either as a dimer or a trimer. The two- to three-fold reorganization and fold-back of VP5* may be linked to membrane penetration, by exposing its hydrophobic region.SAAS annotation

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	49 – 208	VP4_haemagglutInterPro annotationAdd BLAST	160
Domainⁱ	234 – 458	Rota_VP4_MIDInterPro annotationAdd BLAST	225
Domainⁱ	469 – 708	VP4_helicalInterPro annotationAdd BLAST	240

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	468 – 495	Sequence analysisAdd BLAST		28

Sequence similaritiesⁱ

Belongs to the rotavirus VP4 family.UniRule annotationSAAS annotation

Keywords - Domainⁱ

Coiled coilSequence analysisSAAS annotation

Family and domain databases

InterProⁱ	View protein in InterPro IPR013320. ConA-like_dom_sf. IPR035330. Rota_VP4_MID. IPR038017. Rota_VP4_MID_sf. IPR000416. VP4_concanavalin-like. IPR035329. VP4_helical.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF17477. Rota_VP4_MID. 1 hit. PF00426. VP4_haemagglut. 1 hit. PF17478. VP4_helical. 1 hit.
SUPFAMⁱ	SSF111379. SSF111379. 1 hit. SSF49899. SSF49899. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Fragment.

A0A161I2Q8-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
VVLSDDTRIG STKTQNVTIN PGPFAQTGYA PVNWGPGETN DSTTVEPVLD 
        60         70         80         90        100
GPYQPTTFNP PVDYWMLLAP TAPGVVVEGT NNTDRWLATI LVEPSVPSEI 
       110        120        130        140        150
RSYTIFGIQE QITVANTSQT QWKFIDVVKT AQGGSYSQYG PLQSTPKLYA 
       160        170        180        190        200
VMKHNGKIYT YNGETPNATT GYYSTTNYDS VNMTAFCDFY IIPRAEESTC 
       210        220        230        240        250
TEYINNGLPP IQNTRNIVPL ALSARSIISP RAQANEDIVV SKTSLWKEMQ 
       260        270        280        290        300
YNRDITIRFK FANSIIKSGG LGYKWSEISF KPANYQYTYT RDGEEVTAHT 
       310        320        330        340        350
TCSVNGMNDF NFNGGSLPTD FVISRYEVIK ENSFVYVDYW DDSQAFKNMV 
       360        370        380        390        400
YVRSLAANLN SVMCTGGDYS FALPVGQWPV MTGGAVSLHS AGVTLSTQFT 
       410        420        430        440        450
DFVSLNSLRF RFRLTVEEPS FSITRTRVSR LYGLPAANPN NGKEYYEVAG 
       460        470        480        490        500
RFSLISLVPS NDDYQTPIAN SVTVRQDLER QLGELREEFN ALSQEIAMSQ 
       510        520        530        540        550
LIDLALLPLD MFSMFSGIKS TIDAAKSMAT SVMKKFKKSS LASSVSTLTD 
       560        570        580        590        600
SLSDAASSIS RGSSIRSIGS SVSAWTDVST QITDVSSSVS SISTQTSTIS 
       610        620        630        640        650
RRLRLKEMAT QTEGMNFDDI SAAVLKTKID RSTQISPNTL PDIVTEASEK 
       660        670        680        690        700
FIPNRAYRVI NNDEVFEAGT DGRFFAYRVE TFDEIPFDVQ KFADLVTDSP 

VISAIMDLE

Length:709

Mass (Da):78,300

Last modified:July 6, 2016 - v1

Checksum:ⁱEF6582EBE2BFE0C0

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Non-terminal residueⁱ	1	Imported		1
Non-terminal residueⁱ	709	Imported		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	KU243625 Genomic RNA. Translation: ANC33771.1.

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

KU243625 Genomic RNA. Translation: ANC33771.1.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
A0A161I2Q8	A0A168GWW3	Rotavirus G3	709	UniRef100_A0A161I2Q8	Cluster: Outer capsid protein VP4 (Fragment)	2

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
A0A161I2Q8	P12473 A0A1V0FU06 A0A1V0FUK0 A0A1V0FUR9 Q2PZB8 A0A168GWZ8 A0A168GWW3	Rotavirus A (strain RVA/Monkey/United States/RRV/1980/G3P5B[3]) (RV-A) Rotavirus A Human rotavirus A strain CMH222 Rotavirus G3	776	UniRef90_P12473	Cluster: Outer capsid protein VP4	8

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
A0A161I2Q8	P12473 A0A1V0FUR9 A0A1V0FUK0 A0A1V0FU06 Q2PZB8 A0A168GWZ8 A0A168GWW3 U5LY07 H9ZIN3 G1CDB3 +702	Rotavirus A (strain RVA/Monkey/United States/RRV/1980/G3P5B[3]) (RV-A) Rotavirus A Human rotavirus A strain CMH222 Rotavirus G3 Porcine rotavirus Porcine rotavirus A Rotavirus A RVA/Human-wt/THA/KKL-117/2014/G9P[23] Rotavirus A RVA/Pig-wt/ITA/2CR/2009/G9P[23] Rotavirus A RVA/Pig-wt/ITA/7RE/2009/G9P[23] Rotavirus A RVA/Pig-wt/ITA/3BS/2009/G9P[23] And more	776	UniRef50_P12473	Cluster: Outer capsid protein VP4	713

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	KU243625 Genomic RNA. Translation: ANC33771.1.

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

KU243625 Genomic RNA. Translation: ANC33771.1.

3D structure databases

ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Family and domain databases

InterProⁱ	View protein in InterPro IPR013320. ConA-like_dom_sf. IPR035330. Rota_VP4_MID. IPR038017. Rota_VP4_MID_sf. IPR000416. VP4_concanavalin-like. IPR035329. VP4_helical.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF17477. Rota_VP4_MID. 1 hit. PF00426. VP4_haemagglut. 1 hit. PF17478. VP4_helical. 1 hit.
SUPFAMⁱ	SSF111379. SSF111379. 1 hit. SSF49899. SSF49899. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	A0A161I2Q8_9REOV
Accessionⁱ	A0A161I2Q8Primary (citable) accession number: A0A161I2Q8
Entry historyⁱ	Integrated into UniProtKB/TrEMBL:	July 6, 2016
	Last sequence update:	July 6, 2016
	Last modified:	February 28, 2018
	This is version 11 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Unreviewed (UniProtKB/TrEMBL)

UniProtKB - A0A161I2Q8 (A0A161I2Q8_9REOV)

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Outer capsid protein VP4

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Functionⁱ

GO - Biological processⁱ

Keywordsⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Interactionⁱ

Subunit structureⁱ

Family & Domainsⁱ

Domains and Repeats

Coiled coil

Sequence similaritiesⁱ

Keywords - Domainⁱ

Family and domain databases

Sequenceⁱ

Experimental Info

Sequence databases

Similar proteinsⁱ

Cross-referencesⁱ

Sequence databases

3D structure databases

Protocols and materials databases

Family and domain databases

Entry informationⁱ

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - A0A161I2Q8 (A0A161I2Q8_9REOV)

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Outer capsid protein VP4

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Coiled coil

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Family and domain databases

Experimental Info

Sequence databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protocols and materials databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

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Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Interactionⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ