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Protein

ATP-dependent RNA helicase DbpA

Gene

dbpA

Organism
Escherichia coli (strain B / BL21-DE3)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

DEAD-box RNA helicase involved in the assembly of the 50S ribosomal subunit. Has an RNA-dependent ATPase activity, which is specific for 23S rRNA, and a 3' to 5' RNA helicase activity that uses the energy of ATP hydrolysis to destabilize and unwind short rRNA duplexes.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHelicaseUniRule annotationImported, Hydrolase, RNA-bindingUniRule annotation
Biological processRibosome biogenesisUniRule annotation
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DbpAUniRule annotation (EC:3.6.4.13UniRule annotation)
Gene namesi
Name:dbpAUniRule annotation
Ordered Locus Names:ECBD_2273Imported
OrganismiEscherichia coli (strain B / BL21-DE3)Imported
Taxonomic identifieri469008 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000002032 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA0A140NC60.
SMRiA0A140NC60.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 31Q_MOTIFInterPro annotationAdd BLAST29
Domaini34 – 205Helicase ATP-bindingInterPro annotationAdd BLAST172
Domaini230 – 376Helicase C-terminalInterPro annotationAdd BLAST147

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni383 – 457Involved in 23S rRNA bindingUniRule annotationAdd BLAST75

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi3 – 31Q motifPROSITE-ProRule annotationAdd BLAST29

Domaini

Contains an N-terminal domain that binds non-specifically to RNA and a C-terminal domain that binds specifically and tightly to hairpin 92 of 23S rRNA.UniRule annotation

Sequence similaritiesi

Belongs to the DEAD box helicase family. DbpA subfamily.UniRule annotation

Phylogenomic databases

KOiK05591.
OMAiFGCQALV.

Family and domain databases

HAMAPiMF_00965. DEAD_helicase_DbpA. 1 hit.
InterProiView protein in InterPro
IPR005580. DbpA/CsdA_RNA-bd_dom.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR028619. DEAD_helicase_DbpA.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
PfamiView protein in Pfam
PF03880. DbpA. 1 hit.
PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A140NC60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAFSTLNVL PPAQLTNLNE LGYLTMTPVQ AAALPAILAG KDVRVQAKTG
60 70 80 90 100
SGKTAAFGLG LLQQIDASLF QTQALVLCPT RELADQVAGE LRRLARFLPN
110 120 130 140 150
TKILTLCGGQ PFGMQRDSLQ HAPHIIVATP GRLLDHLQKG TVSLDALNTL
160 170 180 190 200
VMDEADRMLD MGFSDAIDDV IRFAPASRQT LLFSATWPEA IAAISGRVQR
210 220 230 240 250
DPLAIEIDST DALPPIEQQF YETSSKGKIP LLQRLLSLHQ PSSCVVFCNT
260 270 280 290 300
KKDCQAVCDA LNEVGQSALS LHGDLEQRDR DQTLVRFANG SARVLVATDV
310 320 330 340 350
AARGLDIKSL ELVVNFELAW DPEVHVHRIG RTARAGNSGL AISFCAPEEA
360 370 380 390 400
QRANIISDML QIKLNWQTPP ANSSIATLEA EMATLCIDGG KKAKMRPGDV
410 420 430 440 450
LGALTGDIGL DGADIGKIAV HPAHVYVAVR QAVAHKAWKQ LQGGKIKGKT

CRVRLLK
Length:457
Mass (Da):49,188
Last modified:May 11, 2016 - v1
Checksum:iD9198B1FABA4D38A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001665 Genomic DNA. Translation: ACT29301.1.
RefSeqiWP_000123737.1. NC_012971.2.

Genome annotation databases

EnsemblBacteriaiACT29301; ACT29301; ECBD_2273.
KEGGiebd:ECBD_2273.
ebe:B21_01330.
ebl:ECD_01320.
PATRICifig|469008.15.peg.1359.

Similar proteinsi

Entry informationi

Entry nameiA0A140NC60_ECOBD
AccessioniPrimary (citable) accession number: A0A140NC60
Entry historyiIntegrated into UniProtKB/TrEMBL: May 11, 2016
Last sequence update: May 11, 2016
Last modified: June 7, 2017
This is version 10 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported