Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Fumarate hydratase class II

Gene

aspA

Organism
Mycobacterium wolinskyi
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (aspA)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei190SubstrateUniRule annotation1
Active sitei191Proton donor/acceptorUniRule annotation1
Active sitei322UniRule annotation1
Binding sitei323SubstrateUniRule annotation1
Sitei335Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotationImported
Biological processTricarboxylic acid cycleUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:aspAImported
Synonyms:fumCUniRule annotation
ORF Names:AFM11_04935Imported, AWC31_20215Imported
OrganismiMycobacterium wolinskyiImported
Taxonomic identifieri59750 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000070612 Componenti: Unassembled WGS sequence
  • UP000193964 Componenti: Unassembled WGS sequence

Subcellular locationi

A0A132PTI9:
  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 346Lyase_1InterPro annotationAdd BLAST325
Domaini412 – 468FumaraseC_CInterPro annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni108 – 110Substrate bindingUniRule annotation3
Regioni132 – 135Substrate binding (B site)UniRule annotation4
Regioni142 – 144Substrate bindingUniRule annotation3
Regioni328 – 330Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Family and domain databases

CDDicd01362. Fumarase_classII. 1 hit.
Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC. 1 hit.
InterProiView protein in InterPro
IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiView protein in Pfam
PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
PROSITEiView protein in PROSITE
PS00163. FUMARATE_LYASES. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A132PTI9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDKAVDASG EGEYRIEHDT MGEVRVPVNA LWRAQTQRAV ENFPISFRGL
60 70 80 90 100
ERTQIRALGL LKAACAQVNK DLGLLAPEKA DAIIAAAGEI ADGQHDDQFP
110 120 130 140 150
IDVFQTGSGT SSNMNTNEVI ASIAARNGVT VHPNDDVNMS QSSNDTFPTA
160 170 180 190 200
THIAATEAAV RHLIPALEVL HESLAAKARQ WRTVVKSGRT HLMDAVPVTL
210 220 230 240 250
GQEFGGYARQ IEAGIERVKA TLPRLGELAI GGTAVGTGLN APDGFGAKVV
260 270 280 290 300
EVLVNQTGLA ELRTAVDSFE AQAARDGLVE ASGALRTIAV SLTKIANDVR
310 320 330 340 350
WMGSGPLTGL AEIQLPDLQP GSSIMPGKVN PVLPEAVTQV ACQVVGNDAA
360 370 380 390 400
IAMGGASGAF ELNVYIPMMA RNLLESFTLL SNVSKLFAAR CIDGLVANEE
410 420 430 440 450
HLRELAESSP SIVTPLNSAI GYEEAAKVAK QALKEKKTIR QTVIDRGLIG
460 470
DKLSEAELDK RLDVLAMAKV KDGD
Length:474
Mass (Da):50,134
Last modified:June 8, 2016 - v1
Checksum:iCD52186217C8B145
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LGTW01000002 Genomic DNA. Translation: KWX25584.1.
LQQA01000010 Genomic DNA. Translation: ORX16383.1.
RefSeqiWP_067844682.1. NZ_LQQA01000010.1.

Genome annotation databases

EnsemblBacteriaiKWX25584; KWX25584; AFM11_04935.
PATRICifig|59750.3.peg.2868.

Similar proteinsi

Entry informationi

Entry nameiA0A132PTI9_9MYCO
AccessioniPrimary (citable) accession number: A0A132PTI9
Entry historyiIntegrated into UniProtKB/TrEMBL: June 8, 2016
Last sequence update: June 8, 2016
Last modified: September 27, 2017
This is version 12 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteome, Reference proteomeImported