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Protein

Fumarate hydratase class II

Gene

aspA

Organism
Mycobacterium sp. IS-1742
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (aspA)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei189SubstrateUniRule annotation1
Active sitei190Proton donor/acceptorUniRule annotation1
Active sitei321UniRule annotation1
Binding sitei322SubstrateUniRule annotation1
Sitei334Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotation
Biological processTricarboxylic acid cycleUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:aspAImported
Synonyms:fumCUniRule annotation
ORF Names:AU196_14370Imported
OrganismiMycobacterium sp. IS-1742Imported
Taxonomic identifieri1772285 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000054128 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 345Lyase_1InterPro annotationAdd BLAST325
Domaini411 – 467FumaraseC_CInterPro annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni107 – 109Substrate bindingUniRule annotation3
Regioni131 – 134Substrate binding (B site)UniRule annotation4
Regioni141 – 143Substrate bindingUniRule annotation3
Regioni327 – 329Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Family and domain databases

CDDicd01362. Fumarase_classII. 1 hit.
Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC. 1 hit.
InterProiView protein in InterPro
IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiView protein in Pfam
PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
PROSITEiView protein in PROSITE
PS00163. FUMARATE_LYASES. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A101AXA5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTADSAVDQN TEYRIEHDTM GEVRVPKDAL WRAQTQRAVE NFPISGRGLE
60 70 80 90 100
RTQIRALGLL KGACAQVNKD LGLLDAEKAD AIIAAAAEIA DGQHDDQFPI
110 120 130 140 150
DVFQTGSGTS SNMNTNEVIA SIAAANGVTV HPNDHVNMSQ SSNDTFPTAT
160 170 180 190 200
HIAATEAAVR HLIPALEVLH ESLAGKARQW RTVVKSGRTH LMDAVPVTLG
210 220 230 240 250
QEFGGYARQI EAGIERVRGS LPRLGELAIG GTAVGTGLNA PDGFGAKVVQ
260 270 280 290 300
VLVDQTGLGE LTTAKDSFEA QAARDGLVEA SGALRVIAVS LTKIANDVRW
310 320 330 340 350
MGSGPLTGLA EIQLPDLQPG SSIMPGKVNP VLPEAVTQVA AQVIGNDAAV
360 370 380 390 400
AWGGAAGAFE LNVYIPMMAR NLLESFKLLT NVSRLFAERC IDGLVANEAH
410 420 430 440 450
MREQAESSPS IVTPLNSAIG YEEAAKVAKE ALKERKTIRQ TVIDRGLIGD
460 470
KLSEEELDRR LDVLAMAKVK DGD
Length:473
Mass (Da):50,183
Last modified:April 13, 2016 - v1
Checksum:iF7A53FCF02C1157B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LQIV01000008 Genomic DNA. Translation: KUI30689.1.
RefSeqiWP_059094375.1. NZ_LQIV01000008.1.

Genome annotation databases

EnsemblBacteriaiKUI30689; KUI30689; AU196_14370.

Similar proteinsi

Entry informationi

Entry nameiA0A101AXA5_9MYCO
AccessioniPrimary (citable) accession number: A0A101AXA5
Entry historyiIntegrated into UniProtKB/TrEMBL: April 13, 2016
Last sequence update: April 13, 2016
Last modified: September 27, 2017
This is version 10 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteomeImported